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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2006, Vol. 7 Issue (11): 9-    DOI: 10.1631/jzus.2006.B0899
    
Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells
LI Yi, DU Bin, PAN Jia-qi, CHEN De-chang, LIU Da-wei
Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Medical Academy, Beijing 100730, China; Department of Emergency Medicine, Peking Union Medical College Hospital, Chinese Medical Academy, Beijing 100730, China; Department of Hematology, Peking Union Medical College Hospital, Chinese Medical Academy, Beijing 100730, China
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Abstract  Objective: To investigate the effect of activated protein C (APC) on inflammatory responses in human umbilical vein endothelial cells (HUVEC) stimulated with lipopolysaccharide (LPS). Methods: The second passage of collagenase digested HUVEC was divided into the following groups: serum free medium control group (SFM control), phosphate buffer solution control group (PBS control), LPS group with final concentration of 1 μg/ml (LPS group), APC group with final concentration of 7 μg/ml, Pre-APC group (APC pretreatment for 30 min prior to LPS challenge), and Post-APC group (APC administration 30 min after LPS challenge). Supernatant was harvested at 0, 4, 8, 12 and 24 h after LPS challenge. Interleukin-6 (IL-6) and Interleukin-8 (IL-8) levels were analyzed with ELISA. Cells were harvested at 24 h after LPS challenge, and total RNA was extracted. Messenger RNA levels for IL-6 and IL-8 were semi-quantitatively determined by RT-PCR. Results: Compared with control group, IL-6 and IL-8 levels steadily increased 4 to 24 h after LPS stimulation. APC treatment could increase LPS-induced IL-6 and IL-8 production. The mRNA levels of IL-6 and IL-8 exhibited a similar change. Conclusion: APC can further increase the level of IL-6 and IL-8 induced by LPS. The effect of these elevated cytokines is still under investigation.

Key words： Activated protein C (APC)      Interleukin-6 (IL-6)      Interleukin-8 (IL-8)      Sepsis      Human umbilical vein endothelial cell (HUVEC)     
Received: 12 July 2006     
CLC:  R55  
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LI Yi
DU Bin
PAN Jia-qi
CHEN De-chang
LIU Da-wei
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LI Yi, DU Bin, PAN Jia-qi, CHEN De-chang, LIU Da-wei. Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(11): 9-.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2006.B0899     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2006/V7/I11/9

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