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| A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues |
| LI Jing, ZHAO Hai-yan, RUAN Xing-yun, XU Yong-qing, MENG Wei-zheng, LI Kun-peng, ZHANG Jing-qiang |
| Department of Equipments, Kunming General Hospital, Kunming 650032, China; State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510275, China; Department of Ophthalmology, Yunnan Province People’s Hospital, Kunming 650032, China; Department of Orthopaedics, Kunming General Hospital, Kunming 650032, China |
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Abstract A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fourier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform simultaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction segmentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.
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Received: 26 February 2005
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