Please wait a minute...
ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2002, Vol. 3 Issue (2): 216-222    DOI: 10.1631/jzus.2002.0216
Biotechnology & Life Sciences     
cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus
ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  Genome segments S8 of two Chinese isolates of rice black-streaked dwarf virus (RBSDV), one from Zhejiang Province and another from Hebei Province, were amplified by RT-PCR and sequenced. Both segments consisted of 1936 nts in full length (EMBL accession numbers were AJ297431 and AJ297432, respectively) and contained only one big open reading frame which encoded a polypeptide with molecular weight of 68kD. The two Chinese isolates shared 94.0% and 96.5% identity at nucleotide and amino acid level, respectively. They shared 94.5-94.9% and 92.5-92.9% homology with S8 of RBSDV Japanese isolate at nucleotide and amino acid level, respectively; shared 85.1-87.6% and 91.7-91.9% homology with S7 of Italian MRDV (maize rough dwarf virus).

Key words： Rice black-streaked dwarf virus(RBSDV)      Genome segment S8      Sequence analysis      Biotechnology     
Received: 18 July 2001     
CLC:  S432.41  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
CHEN Jian-ping
ZHANG Heng-mu
XUE Qing-zhong
LEI Juan-li
Cite this article:

ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li. cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(2): 216-222.

URL:

http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2002.0216     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2002/V3/I2/216

[1] LIANG Wei-feng, SHEN Yue-hong, MA Yi-lin, ZHAO Nian-feng, CHEN Ya-gang, ZHU Zhi-yong, XU Xiao. MOLECULAR CLONING AND NUCLEOTIDES SEQUENCE ANALYSIS OF G1 GENOME SEGMENT OF HANTAAN VIRUS Z10 STRAIN[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2000, 1(2): 207-211.