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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2003, Vol. 4 Issue (1): 114-120    DOI: 10.1631/jzus.2003.0114
Biomedicine     
Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits
HUANG Xian-mei, ZHU Wei-hua, KANG Man-li
Children\'s Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China
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Abstract  To investigate the effect of doxorubicin (DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR) Ca2+ transport proteins and the mechanism of taurine (Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX, 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function, concentration of calcium in cardiomyocytes (Myo[Ca2+]i), activity of SR Ca2+-ATPase(SERCA2a), level of SERCA2a mRNA and Ca2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca2+-ATPase and level of SERCA2a mRNA decreased, while Myo[Ca2+]i increased in DOX-treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca2+]i and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX-induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.

Key words： Doxorubicin      Ca2+-ATPase      Ryanodine receptor      Taurine      Gene expression     
Received: 28 December 2001     
CLC:  R725.4  
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HUANG Xian-mei
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KANG Man-li
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HUANG Xian-mei, ZHU Wei-hua, KANG Man-li. Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2003, 4(1): 114-120.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2003.0114     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2003/V4/I1/114

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