Please wait a minute...
ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2004, Vol. 5 Issue (6): 644-650    DOI: 10.1631/jzus.2004.0644
Biotechnology & Food Science     
Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination
WU Xiao-feng, CAO Cui-ping
Lab of Gene Engineering, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination.

Key words： Gene targeting      Recombinant vector      Bombyx mori L.     
Received: 29 October 2003     
CLC:  S881.2  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
WU Xiao-feng
CAO Cui-ping
Cite this article:

WU Xiao-feng, CAO Cui-ping. Targeting of human aFGF gene into silkworm, Bombyx mori L. through homologous recombination. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(6): 644-650.

URL:

http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2004.0644     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2004/V5/I6/644

[1] MIAO Yun-gen, JIANG Li-jun, Bharathi D. EFFECTS OF JINLU, AN ANTI-JUVENILE HORMONE ON THE GROWTH, ULTRA-STRUCTURE OF THE CORPORA ALLATA AND PROTHORACIC GLAND OF SILKWORM, BOMBYX MORI L[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2001, 2(3): 294-297.
[2] Yao Hui-Peng, Wu Xiao-Feng, Gokulamma K.. Antiviral activity in the mulberry silkworm, Bombyx mori L.[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2006, 7(Supplement 2): 350-356.
[3] Yao Hui-Peng, Wu Xiao-Feng, Gokulamma K.. Antiviral activity in the mulberry silkworm, Bombyx mori L.[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 0, 7(101): 350-356.