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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2004, Vol. 5 Issue (10): 1199-1205    DOI: 10.1631/jzus.2004.1199
Life Science & Biotechnology     
Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.
YAN Cheng-qi, QIAN Kai-xian, XUE Gang-ping, WU Zhong-chang, CHEN Yue-lei, YAN Qiu-sheng, ZHANG Xue-qing, Wu Ping
College of Life Sciences, Zhejiang University, Hangzhou 310029, China; Ningbo Academy of Agriculture Sciences, Ningbo 315040, China; China National Rice Research Institute, Hangzhou 310006, China; CSIRO Plant Industry 306 Carmody Rd., St Lucia, Qld 4067, Australia
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Abstract  Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.

Key words： Oryza sativa L.      Oryza meyeriana L.      Somatic hybridization      Rice bacterial blight resistance     
Received: 07 March 2004     
CLC:  Q943  
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QIAN Kai-xian
YAN Cheng-qi
XUE Gang-ping
WU Zhong-chang
CHEN Yue-lei
YAN Qiu-sheng
ZHANG Xue-qing
Wu Ping
Cite this article:

YAN Cheng-qi, QIAN Kai-xian, XUE Gang-ping, WU Zhong-chang, CHEN Yue-lei, YAN Qiu-sheng, ZHANG Xue-qing, Wu Ping. Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(10): 1199-1205.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2004.1199     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2004/V5/I10/1199

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