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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2003, Vol. 4 Issue (3): 331-335    DOI: 10.1631/jzus.2003.0331
Chemical and Biotechnology     
Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa
JIANG Hua-wu, DIAN Wei-min, LIU Fei-yan, WU Ping
The State Key Laboratory of Plant Physiology and Biochemistry, College of Life sciences, Zhejiang University, Hangzhou 310029, China
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Abstract  Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator (GPT) was isolated from a cDNA library of immature seeds of rice and named as OsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of 70 amino acid residues. The OsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression of OsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported via GPT can be used for starch biosynthesis in rice nongreen plastids.

Key words： Glucose 6-phosphate/phosphate translocator      Starch synthesis      Rice (Oryza sativa L.)     
Received: 26 June 2002     
CLC:  Q943.2  
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JIANG Hua-wu
DIAN Wei-min
LIU Fei-yan
WU Ping
Cite this article:

JIANG Hua-wu, DIAN Wei-min, LIU Fei-yan, WU Ping. Cloning and characterization of a glucose 6-phosphate/phosphate translocator from Oryza sativa. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2003, 4(3): 331-335.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2003.0331     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2003/V4/I3/331

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