Please wait a minute...
ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2003, Vol. 4 Issue (3): 336-339    DOI: 10.1631/jzus.2003.0336
Chemical and Biotechnology     
Emergence of a new satellite RNA from cucumber mosaic virus isolate P1
Sandra Pérez Alvarez, XUE Chao-yang, ZHOU Xue-ping
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  The cucumber mosaic virus (CMV) isolate P1 caused very mild symptoms on many plant species. After serial passages by mechanical inoculation over five years, CMV P1 caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT-PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. Sequence analysis showed there were two satRNAs (Sat-P1-1 and Sat-P1-2). Sat-P1-1 contained 335 nucleotides, and Sat-P1-2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat-P1-1 was identical to a satRNA (Z96099) reported in 1995 in CMV P1. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat-P1-2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.

Key words： Cucumber mosaic virus      Satellite RNA      Sequence     
Received: 02 August 2002     
CLC:  S432.41  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Sandra Pérez Alvarez
XUE Chao-yang
ZHOU Xue-ping
Cite this article:

Sandra Pérez Alvarez, XUE Chao-yang, ZHOU Xue-ping. Emergence of a new satellite RNA from cucumber mosaic virus isolate P1. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2003, 4(3): 336-339.

URL:

http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2003.0336     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2003/V4/I3/336

[1] Zhi-feng Zhang, Yi-xiong Feng, Jian-rong Tan, Wei-qiang Jia, Guo-dong Yi. A novel approach for parallel disassembly design based on a hybrid fuzzy-time model[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2015, 16(9): 724-736.
[2] Yusuke Bota, Hajime Mizuyama, Akio Noda, Tatsuya Nagatani, Ken-ichi Tanaka. A tree-shaped motion strategy for robustly executing robotic assembly tasks[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2010, 11(12): 986-991.
[3] Zhen LU, Jian-hua GE. Time-domain training sequences design for MIMO OFDM channel estimation[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2008, 9(4): 464-469.
[4] Jia-qiang YANG, Jin HUANG, Tong LIU. Diagnosis of stator faults in induction motor based on zero sequence voltage after switch-off[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2008, 9(2): 165-172.
[5] Bao-jun ZHANG, Xue-zeng PAN, Jie-bing WANG, Ling-di PING. A recoverable stress testing algorithm for compression and encryption cards[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2008, 9(10): 1398-1405.
[6] LIU Guang-hui, CHEN Chuan-bo. A new algorithm for computing the convex hull of a planar point set[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2007, 8(8): 1210-1217.
[7] ZHANG Ming-kui, ZHENG Shun-an. Competitive adsorption of Cd, Cu, Hg and Pb by agricultural soils of the Changjiang and Zhujiang deltas in China[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2007, 8(11): 1808-1815.
[8] SHI Mei-hua. Almost split sequences for symmetric non-semisimple Hopf algebras[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2006, 7(6 ): 22-.
[9] LIN Zheng-yan, SHEN Xin-mei. A note on strong law of large numbers of random variables[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2006, 7(6): 1088-1091.
[10] ASMA BEKTAŞ Çiğdem. On some difference sequence spaces defined by a sequence of Orlicz functions[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2006, 7(12): 21-.
[11] SONG Bo-wei, GUAN Yun-feng, ZHANG Wen-jun. An efficient training sequences strategy for channel estimation in OFDM systems with transmit diversity[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6( 6): 23-.
[12] REN Kui, PARK Jaemin, KIM Kwangjo. On the construction of cryptographically strong Boolean functions with desirable trade-off[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2005, 6( 5): 2-.
[13] MAO Chuan-zao, YANG Ling, ZHENG Bing-song, WU Yun-rong, LIU Fei-yan, YI Ke-ke, WU Ping. Comparative mapping of QTLs for Al tolerance in rice and identification of positional Al-induced genes[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(6): 634-643.
[14] ZHONG Bo-xiong, WENG Hong-biao, FANG Wei-huan. Preparation of protein samples for gel electrophoresis by sequential extraction[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(5): 606-610.
[15] ZHANG Heng-mu, CHEN Jian-ping, XUE Qing-zhong, LEI Juan-li. cDNA cloning and sequence analysis of genome segments S8 from rice black-streaked dwarf virus[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(2): 216-222.