| Science Letters |
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| Enhancing rice resistance to fungal pathogensby transformation with cell wall degrading enzyme genes from Trichoderma atroviride |
| LIU Mei, SUN Zong-xiu, ZHU Jie, XU Tong, HARMAN Gary E., LORITO Matteo |
| College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; Key Laboratory for Rice Biology, Ministry of Agriculture, China National Rice Research Institute, Hangzhou 310006, China; Department of Horticultural Sciences, Cornell University, Geneva, USA; Institute of Plant Pathology, Napoli University, Tritici, Italy |
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Abstract Three genes encoding for fungal cell wall degrading enzymes (CWDEs), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants. More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance to Magnaporthe grisea was found in all kinds of regenerated plants including that with single gluc78. A few lines expressing either ech42 or nag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level. This is the first report of multiple of expression of genes encoding CWDEs from Trichoderma atroviride that result in resistance to blast and sheath blight in rice.
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Received: 18 November 2003
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