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Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2004, Vol. 5 Issue (2): 137-143    DOI: 10.1631/jzus.2004.0137
Bioscience & Biotechnology     
Programmed cell death features in apple suspension cells under low oxygen culture
XU Chang-jie, CHEN Kun-song, FERGUSON Ian B.
Laboratory of Fruit Molecular Physiology and Biotechnology / The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development & Biotechnology, Zhejiang University, Hangzhou 310029, China; The Horticulture and Food Research Institute of New Zealand, Private Bag 92 169, Auckland, New Zealand
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Abstract  Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were exposed to a low oxygen atmosphere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Protoplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidylserine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple cell death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit.

Key wordsApple      Programmed cell death      Low oxygen     
Received: 06 December 2003     
CLC:  Q942  
Cite this article:

XU Chang-jie, CHEN Kun-song, FERGUSON Ian B.. Programmed cell death features in apple suspension cells under low oxygen culture. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2004, 5(2): 137-143.

URL:

http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2004.0137     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2004/V5/I2/137

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