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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2003, Vol. 4 Issue (2): 232-235    DOI: 10.1631/jzus.2003.0232
Biomedicine     
Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori
YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang
Department of Microbiology, College of Medicine, Zhejiang University, Hangzhou 310006, China
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Abstract  Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein of H. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic ulcer disease, 2 with gastric cancer) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7),gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ2=13.327, P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (rs=0.442, P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases.

Key words： Helicobacter pylori      CagA      Elution from gel      Western-blot     
Received: 28 December 2001     
CLC:  R377  
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YE Shao-jing
FANG Ping-chu
MAO Guo-gen
LI Chao-long
Qiu Xiang
CHEN Hai-xiang
Cite this article:

YE Shao-jing, FANG Ping-chu, MAO Guo-gen, LI Chao-long, Qiu Xiang, CHEN Hai-xiang. Purification and relationship with gastric disease of a 130 kDa (CagA) protein of Helicobacter pylori. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2003, 4(2): 232-235.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2003.0232     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2003/V4/I2/232

[1] WU Qin-dong, ZHU Yong-liang, SHI Yi-hai. Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(5): 627-631.
[2] WU Qin-dong, ZHU Yong-liang. Study of serum Helicobacter pylori soluble antigen[J]. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(4): 484-488.