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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2002, Vol. 3 Issue (4): 484-488    DOI: 10.1631/jzus.2002.0484
Biotechnology & Life Sciences     
Study of serum Helicobacter pylori soluble antigen
WU Qin-dong, ZHU Yong-liang
Department of Gastroenterology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China
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Abstract  Objective: to explore a new serological method for detecting Helicobac pylori (H. pylori) infection. Methods: Serum soluble antigen of H. pylori was detected by using avidin-biotin ELISA technique to evaluate the status of H. pylori infection and for comparison with rapid urease test (RUT), histologic examination and serology. Results: The sensitivity, specificity, positive predictive value and negative predictive value were 77.46%, 91.07%, 91.67% and 76.12%, respectively. The prevalence rate of serum H. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by 14C-UBT methods (P>0.05). Conclusions: The detection of serum H. pylori soluble antigen (HpSAg) could be used as a new serological method which is accurate, and convenient, not affected by the memorizing reaction of serum antibody; is more sensitive, more specific and suitable for clinical diagnosis, and evaluation of eradication and for follow-up of H. pylori as well as for detection in children and pregnant women.

Key words： Helicobacter pylori      Antigen      Serology     
Received: 08 August 2001     
CLC:  R573.3  
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WU Qin-dong
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Cite this article:

WU Qin-dong, ZHU Yong-liang. Study of serum Helicobacter pylori soluble antigen. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2002, 3(4): 484-488.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.2002.0484     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2002/V3/I4/484

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