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浙江大学学报(理学版)
生命科学     
草鱼血清凝集素的研究
浙江大学西溪校区生命科学学院,浙江 杭州 310012
Study on an Agglutinin Isolated from Serum of Grass Carp (Ctenopharyngodon idellus).
College of Life Science, Zhejiang University, Hangzhou, 310012,China
 全文: PDF(58 KB)  
摘要: 从草鱼血清分离到一种天然的凝集素分子,能够凝集多种不同的抗原物质,其组成成分为糖蛋白,具有耐热和耐酸碱性,对β-巯基乙醇敏感.凝集反应受温度、反应介质的p H、离子强度以及时间等因素的影响.经糖的专一性抑制实验显示,半乳糖和乳糖对凝集反应的抑制作用最强,甘露糖和N-乙酰葡萄糖胺也有一定的抑制作用,说明这4种糖分子可能在凝集过程中起主要作用.
关键词: 草鱼凝集素理化性质     
Abstract: A natural occuring agglutinin, which was detected from serum of grass carp(Ctenopharyngodon idellus), was found to have the ability of agglutinating different kinds of antigen. It has the characteristic of glycoprotein.The agglutinin was stable when exposure to the heat (60℃) for 1h and acid(p H=2) or alkaline(p H=10) for 24h respectively, but was sensitive to the treatment of 1% β-Mercapto ethanol, its agglutinating activity was influenced by the temperature , concentration of ion , reaction time and p H value of the buffer.The agglutinating activity could be inhibited by the splitting of sugar and lactose, galoctose acetylglucosamine and mannose respectively, the results demonstrated the sugar refered above played an important role in agglutinating reaction.
Key words: grass carp    agglutinin    physical and chemical characteristics
收稿日期: 1998-07-01 出版日期: 2017-11-10
CLC:  Q954  
作者简介: 罗依惠(1970-),女,浙江大学湖滨校区基础医学院助教,硕士,主要从事细胞生物学研究
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引用本文:

罗依惠,邵健忠,项黎新,毛树坚. 草鱼血清凝集素的研究[J]. 浙江大学学报(理学版), .

LUO Yi-hui, SHAO Jian-zhong, XIANG Li-xing, et al . Study on an Agglutinin Isolated from Serum of Grass Carp (Ctenopharyngodon idellus).. Journal of Zhejiang University (Science Edition), .

链接本文:

https://www.zjujournals.com/sci/CN/        https://www.zjujournals.com/sci/CN/Y1999/V26/I2/69

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