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Analysis of aflatoxin B1 in contaminated feed, media, and serum samples of Cyprinus carpio L. by high-performance liquid chromatography |
Pradeepkiran Jangampalli Adi*, ** and Bhaskar Matcha** |
*Division of Animal Biotechnology, Department of Zoology, Sri Venkateswara University, Tirupati 517502, India,
**Garrison Institute on Aging, Texas Tech University of Health Science Centre, Lubbock, USA |
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Abstract
Objectives: Enzyme-linked immunosorbent assay (ELISA)-, thin-layer chromatography (TLC)-, and
highperformance liquid chromatography (HPLC)-sensitive methods were used for the identification
of aflatoxin B1 (AFB1) contaminated fish feed, media, and fish serum samples.
Materials and Methods: The ELISA, TLC, and HPLC methods were validated by the quantitative and
qualitative determination of AFB1 in contaminated fish feed, media, and fish blood serum samples.
Results: The primary identification of AFB1 was carried out with a DOA-ELISA test kit ELISA followed
by TLC with RF
values 0.81, 0.79, 0.81, and 0.80 of AFB1-contaminated fish feed, media, and serum
samples, respectively, compared with AFB1 standard. HPLC results show that the AFB1 levels in
contaminated fish feed, media, and serum samples were 2.6, 2.6, and 2.7 ng/mL, concentrations
respectively. The level of concentrations of AFB1 was almost similar in all three samples, but
slightly higher in the fish serum sample with, 2.7 ng/ml. However, the present method is strongly
recommended for monitoring AFB1 contamination in feed stuffs, especially in fisheries where the
feed is under continuous exposure to moisture.
Conclusions: This method is accurate and more sensitive when compared with routine
conventional AFB1 detection methods, and is highly applicable in aquaculture and fisheries to
screen the mycotoxins in fish feed.
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Received: 08 August 2017
Published: 31 July 2018
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Corresponding Authors:
Bhaskar Matcha, Division of Animal Biotechnology, Department of Zoology, Sri Venkateswara University, Tirupati, India.
E-mail: matchabhaskar2010@gmail.com
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高效液相色谱法分析Cyprinus carpio L.污染饲料、培养基和血清样品中黄曲霉毒素
目的:采用酶联免疫吸附试验(ELISA)、薄层色谱(TLC)和高效液相色谱(HPLC)敏感方法对黄曲霉毒素B1(AFB1)污染的鱼饲料、培养基和鱼类血清样品进行鉴定。
材料与方法:采用酶联免疫吸附法(ELISA)、薄层色谱法和高效液相色谱法对污染鱼类饲料、培养基和鱼类血清样品中AFB1进行定性定量测定。
结果:与AFB1标准进行比较,AFB1的初步鉴定是用DOA- ELISA试剂盒ELISA和TLC测定AFB1污染的鱼饲料、培养基和血清样品的RF值分别为0.81、0.79、0.81和0.80。高效液相色谱分析结果表明,污染鱼饲料、培养基和血清样品中AFB1水平分别为2.6、2.6和2.7 ng mL。在三个样品中AFB1的浓度几乎相同,但鱼血清样品中的浓度为2.7 ng mL。然而,本方法被强烈推荐用于监测饲料中的AFB1污染,特别是在饲料连续暴露于湿气中的渔业中。
结论:与常规AFB1检测方法相比,该方法准确、灵敏,适用于水产养殖和水产养殖,筛选鱼类饲料中的真菌毒素。
关键词:
酶联免疫吸附测定法,
薄层色谱法,
高效液相色谱法,
黄曲霉毒素B1分析
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