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Food Qual Safet
Research Articles     
头孢菌提取物及其制剂在控制三种主要储粮害虫中的潜在用途
Potential use of Clerodendrum capitatum extracts and its formulation for control of three major stored product beetles
Jacobs Mobolade Adesina***, Anjanappa Raghavendra***Yallappa Rajashekar**Thomas Inomisan Ofuya****
*Department of Crop, Soil and Pest Management Technology, Rufus Giwa Polytechnic, Owo, Nigeria**Insect Resources Laboratory, Animal Resources Programme, Institute of Bioresources and Sustainable Development, Department of Biotechnology, Government of India, Imphal, ***Division of Insect Ecology, National Bureau of Agricultural Insect Resources, Bangalore, India, ****Department of Crop, Soil and Pest Management, Federal University of Technology, Akure, Nigeria
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摘要:

【研究目的】研究了头孢提取物对储粮害虫,米曲霉(Sitophilus oryzae),多米根霉(Rhyzopertha dominica)和蓖麻Tribolium castaneum(Herbst)的杀虫活性。

【材料和方法】在索氏装置中,用一系列极性递增的溶剂依次提取100克头孢菌素粉末,并将提取物在旋转蒸发仪上浓缩,使其不含溶剂,将残渣溶解在已知体积的甲醇中,用熏蒸剂测定溶液的杀虫活性和毒性。选择具有最高活性的正己烷提取物制备中草药制剂,并研究其杀虫性能和药害。采用GC-MS和GC/FID联用技术,研究了从头孢菌叶获得的草药的成分。 

【结果】头孢提取物对三种主要储粮害虫的成虫都有很强的驱避活性,在1.58 mg/cm2的浓度下具有显著的驱避作用。正己烷提取物(油)具有较高的熏蒸活性,LC50值在25-32μg / L之间。鉴定出总共14种组分,占配方的97.8%,配方中的主要成分为2-庚酮,3-甲基(33.9%)和六氢甲酰基丙酮(25.3%),然后是3-十二烯-1-铝(10.2%)和8-甲基-1-十一苯(4.9%)。此外,还发现草药制剂对所有三种主要储粮害虫的混合年龄培养物均具有剧毒,在24小时暴露下,当剂量为200μg / L时,其死亡率为80% - 90%,暴露72小时,剂量为200μg / L时死亡率达到100%。

【讨论】结果表明,头孢菌素的提取物及其制剂具有开发成天然杀虫剂或驱虫剂的潜力,可用于防治储粮害虫。

关键词: 熏蒸气相色谱-质谱(GC-MS)草药制剂驱虫剂储粮害虫    
Abstract:

Objective 

Insecticidal activity of Clerodendrum capitatum extracts against the stored product insect pest, Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Tribolium castaneum (Herbst) were investigated. 

Materials and Methods 

Hundred grams of C. capitatum powder was sequentially extracted with a series of solvents of increasing polarity in a Soxhlet apparatus and the extracts were concentrated on rotary evaporator to make it solvent free and the residue dissolved in a known volume of methanol and the solution was assayed for insecticidal activity by the fumigant toxicity. Hexane extract which showed maximum activity was selected for the preparation of herbal formulation and for its insecticidal properties and phytotoxicity. The herbal formulation obtained from leaves of C. capitatum was investigated by GCMS and GC/FID. 

Results 

Clerodendrum capitatum ethyl extract had a very strong repellent activity to adults of all three major stored grain insects and was significantly repellent at a 1.58 mg/cm2. The hexane extract (oil) had high fumigant activity with LC50 values in the range between 25 and 32 μg/L. A total of 14 components representing 97.8% of the formulation were identified and the main compounds in the formulation were found to be 2-Heptanone, 3-methyl (33.9 %) and Hexahydrofarnesyl acetone (25.3%) followed by 3-Dodecen-1-al (10.2%) and 8-Methyl-1-undecene (4.9%). Further, herbal formulation was found to be highly toxic to mixed age cultures of all three major stored grain insect pests which recorded 80%–90% mortality at dose of 200 μg/L in 24 h exposure, where as 100% mortality achieved at dose of 200 μg/L in 72 h exposure, respectively. 

Conclusion

The results indicate that extracts and formulation of C. capitatum has the potential to be developed in to a natural insecticide or repellent for controlling of stored product beetles.

Key words: fumigation    gas chromatography mass spectrometry (GCMS)    herbal formulation    repellence    stored product beetles
收稿日期: 2019-02-22 出版日期: 2019-10-31
通讯作者: Jacobs Mobolade Adesina, Department of Crop, Soil and Pest Management Technology, Rufus Giwa Polytechnic, Owo, Nigeria.     E-mail: moboladesina@rugipo.edu.ng
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Jacobs Mobolade Adesina
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引用本文:

Jacobs Mobolade Adesina, Anjanappa Raghavendra, Yallappa Rajashekar, Thomas Inomisan Ofuya. Potential use of Clerodendrum capitatum extracts and its formulation for control of three major stored product beetles. Food Qual Safet, 10.1093/fqsafe/fyz018.

链接本文:

http://www.zjujournals.com/fqs/CN/10.1093/fqsafe/fyz018        http://www.zjujournals.com/fqs/CN/Y2019/V3/I3/179

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