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Food Qual Safet
Research Articles     
基于乳蛋白的可食用薄膜有效释放活性免疫球蛋白
Edible films based on milk proteins release effectively active immunoglobulins
Pirjo Rantam?ki,* Vuokko Loimaranta,** Erkki Vasara,* Jari Latva-Koivisto,* Hannu Korhonen,* Jorma Tenovuo** and Pertti Marnila*
*Natural Resources Institute Finland, FI-31600 Jokioinen, Finland, **Institute of Dentistry, University of Turku, FI-20014 Turun yliopisto, Finland
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摘要:

目的:本研究的目的是开发基于牛乳中免疫球蛋白(Ig)部分的新型可食用蛋白质膜。蛋白膜可用于保护食品免受微生物、化学和物理损害。我们开发了基于牛乳中免疫球蛋白(Ig)部分的新型可食用蛋白质涂膜。可以从乳制品工业的未充分利用的侧流中获得大量的Ig。据我们所知,Ig部分的这种使用尚未发表。

材料和方法:将牛初乳Ig加入基于各种乳蛋白的可食用薄膜中,研究薄膜的特性,包括Ig和乳链菌肽的溶解度以及薄膜的技术性能。Ig的特异性致龋菌变形链球菌和远缘链球菌通过在分娩前对母牛进行超免疫来产生初乳。

结果:富含乳Ig的部分非常适合作为乳蛋白膜的组分。Ig很快就从胶片中溶解了。用于食品保护的Nisin被用作抗菌肽的模型。Nisin从β-乳球蛋白(β-Ig)和β-Ig / Ig膜中释放出生物活性。与β-Ig膜相比,Nisin在β-Ig / Ig膜中以明显更低的浓度发挥其杀菌作用。Nisin在含有富含Ig的乳清的薄膜中也保持了更好的活性。将富含Ig的乳清掺入薄膜中可增强薄膜的粘附力和拉伸强度。通过使膜更光滑透明,富含Ig的乳清也以剂量依赖性方式对基于商业乳清蛋白浓缩物的膜的外观产生强烈影响,这钟性质在大多数食品和药物应用中都是有利的。

结论:具有生物活性的Ig可以成功地掺入基于乳蛋白的可食用薄膜中并从中释放出来。薄膜中Igs的含量相当程度上影响了这些薄膜的性能。膜中其他蛋白质的组成对Igs的保存性和释放也有一定影响。

关键词: 可食用薄膜免疫球蛋白乳清蛋白β-乳球蛋白乳链菌肽龋齿    
Abstract:
Objectives: The goal of this study was to develop novel compositions of edible protein coatings based on immunoglobulin (Ig) fraction from bovine milk. Protein coatings can be used to protect foods against microbial, chemical, and physical damage. We developed novel compositions of edible protein coatings based on immunoglobulin (Ig) fraction from bovine milk. A lot of Ig could be obtained from under-utilized side streams of dairy industry. To the best of our knowledge, such use of the Ig fraction has not been published earlier.
Materials and Methods: Bovine colostral Ig’s were incorporated in edible films based on various milk proteins and investigated the characteristics of the films including solubility of Ig’s and nisin and on technological properties of films. Ig’s specific to cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus were produced to colostrums by hyperimmunizing cows before parturition.
Results: The milk Ig rich fraction suited well as a component of milk protein films. The Ig’s dissolved from the films very rapidly. Nisin, commoly used for food protection, was used as a model of antimicrobial peptide. Nisin was released biologically active from both β-lactoglobulin (β-lg) and β-lg/Ig films. Nisin exerted its bactericidal effect at clearly lower concentrations in the β-lg/Ig films when compared with β-lg film. Nisin also retained its activity better in film containing Ig-enriched whey. Incorporating Ig-enriched whey into films enhanced adhesion and tensile strength of the films. The Ig-enriched whey also affected strongly on the appearance of films based on commercial whey protein concentrate in a dose-dependent way by making the films more smooth, transparent, and clear which are all favoured properties in most food and pharmaceutical applications.
Conclusions: Biologically active Ig’s can be successfully incorporated in and released from milk protein based edible films. The content of Igs in films affected considerably technological properties of these films. Composition of other proteins in films had effect on preservability and release of Igs.
Key words: edible films    immunoglobulins    whey proteins    β-lactoglobulin    caseinates    nisin    dental caries
收稿日期: 2018-08-10 出版日期: 2019-01-24
通讯作者: Pertti Marnila, Luke, Humppilantie 7, 31600 Jokioinen, Finland.     E-mail: pertti.marnila@luke.fi
作者简介: Pertti Marnila, Luke, Humppilantie 7, 31600 Jokioinen, Finland. E-mail: pertti.marnila@luke.fi
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引用本文:

Pirjo Rantamäki, Vuokko Loimaranta, Erkki Vasara, Jari Latva-Koivisto, Hannu Korhonen, Jorma Tenovuo and Pertti Marnila. Edible films based on milk proteins release effectively active immunoglobulins. Food Qual Safet, 10.1093/fqsafe/fyy027.

链接本文:

http://www.zjujournals.com/fqs/CN/10.1093/fqsafe/fyy027        http://www.zjujournals.com/fqs/CN/Y2019/V3/I1/23

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