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Food Qual Safet
Food Qual Safet
Research Articles     
含1-甲基环丙烯纸对贮藏期间安溪柿膜脂代谢的影响
Influences of 1-methylcyclopropene-containing papers on the metabolisms of membrane lipids in Anxi persimmons during storage
Hui Wang*, **, Guo Chen*, Lili Shi*, Hetong Lin*, **, Yihui Chen*, **, Yifen Lin*, **, Zhongqi Fan*, **
*College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China  **Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou, China
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摘要：

目的：研究1-甲基环丙烯（1-MCP）处理对采后贮藏期间安溪柿膜脂代谢的影响。

材料与方法：安溪柿子（Diospyros kaki L. f. cv. Anxi）用含1.35μl/L 的1-MCP的纸张处理安溪柿子。用电导率仪测定细胞膜透性。用分光光度法测定脂肪氧合酶（LOX）、磷脂酶（PLD）和脂肪酶的活性。采用气相色谱法（GC）测定膜脂肪酸的组成和相对含量。

结果：1-MCP处理的安溪柿子电解质外渗率较低，LOX、PLD和脂肪酶活性较低，不饱和脂肪酸（USFAs）含量较高，USFAs与饱和脂肪酸（SFAs）（U/S）比值较高，USFAs指数（IUFA）较高，但SFAs含量较低。

结论：1-MCP处理可抑制甜柿采后贮藏过程中膜脂的降解和代谢，可能是延缓采后柿子软化的原因。

关键词： 安溪柿子;  膜脂;  膜降解酶;  膜过氧化;  1-甲基环丙烯（1-MCP）    
Abstract:

Objectives

The aim of this work was to analyse the effects of 1-methylcyclopropene (1-MCP) treatment on the metabolisms of membrane lipids in postharvest Anxi persimmons during storage.

Materials and methods

Anxi persimmon (Diospyros kaki L. f. cv. Anxi) fruits were treated by paper containing 1-MCP with a concentration of 1.35 μl/l. The cellular membrane permeability was analysed by the electric conductivity meter. The activities of lipoxygenase (LOX), phospholipase (PLD) and lipase were determined by spectrophotometry. The component and relative amounts of membrane fatty acids were determined using gas chromatograph (GC).

Results

The 1-MCP-treated Anxi persimmons manifested a lower electrolyte leakage rate, lower LOX, PLD and lipase activities, higher levels of unsaturated fatty acids (USFAs), higher ratio of USFAs to saturated fatty acids (SFAs) (U/S), higher index of USFAs (IUFA), but lower levels of SFAs.

Conclusions

The degradation and the metabolisms of membrane lipids could be suppressed by 1-MCP treatment, which might be accountable for the delaying softening of postharvest Anxi persimmons during storage.

Key words: Anxi persimmon    membrane lipids    membrane-degrading enzymes    membrane peroxidation    1-methylcyclopropene (1-MCP)
通讯作者: Hetong Lin     E-mail: hetonglin@163.com
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Hui Wang
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引用本文:

Hui Wang, Guo Chen, Lili Shi, Hetong Lin, Yihui Chen, Yifen Lin, Zhongqi Fan. Influences of 1-methylcyclopropene-containing papers on the metabolisms of membrane lipids in Anxi persimmons during storage. Food Qual Safet, 10.1093/fqsafe/fyaa021.

链接本文:

http://www.zjujournals.com/fqs/CN/10.1093/fqsafe/fyaa021    或    http://www.zjujournals.com/fqs/CN/Y2020/V4/I3/143

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