Physiological and biochemical indexes and waxy gene expression of wax-deficient mutant in barley under drought stress

doi:10.3785/j.issn.1008-9209.2018.02.012

Journal of Zhejiang University (Agriculture and Life Sciences)

2019, Vol. 45

Issue (1): 8-13 DOI: 10.3785/j.issn.1008-9209.2018.02.012

Crop cultivation & physiology

Physiological and biochemical indexes and waxy gene expression of wax-deficient mutant in barley under drought stress

Hao ZHENG(

),Xiachen Lü,Saiqiong TAN,Xueli LU,Xian ZHANG,Xiaoqin ZHANG(

),Dawei XUE(

)

1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China

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Abstract

The wild-type (WT) barley ZJU3 and wax-deficient mutant P1 were used as experimental materials. Using the solution culture methods, when the seedlings growing to two leaves, drought stress was simulated by different concentrations of PEG (macrogol)-6000 to compare the physiological and biochemical indexes of the mutant and wild type seedlings. At the same time, the expressions of 10 waxy-related genes were detected by real-time polymerase chain reaction (PCR). The results showed that under drought stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) increased first and then decreased, and the proline (Pro) content increased continuously, and the malondialdehyde (MDA) content increased, indicating that the loss of barley wax powder would reduce its drought resistance. Among the 10 waxy genes selected, P1 had six down-regulated genes with respect to ZJU3, and three genes were up-regulated. Only one gene expression was not significantly different. The study preliminarily reveals the characteristics of the mutant and its advantages in drought resistance.

Key words： barley drought stress physiological and biochemical index waxy gene expression pattern

Received: 01 February 2018 Published: 28 March 2019

CLC:	S 512.3
	Q 945

Corresponding Authors: Xiaoqin ZHANG,Dawei XUE E-mail: 1074137182@qq.com;xiaoqinzhang@163.com;dwxue@126.com

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	Hao ZHENG
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	Xueli LU
	Xian ZHANG
	Xiaoqin ZHANG
	Dawei XUE

Cite this article:

Hao ZHENG,Xiachen Lü,Saiqiong TAN,Xueli LU,Xian ZHANG,Xiaoqin ZHANG,Dawei XUE. Physiological and biochemical indexes and waxy gene expression of wax-deficient mutant in barley under drought stress. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(1): 8-13.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2018.02.012 OR http://www.zjujournals.com/agr/Y2019/V45/I1/8

干旱胁迫下大麦蜡质缺失突变体的生理生化指标及蜡质基因表达

以野生型大麦品种浙农大3号（ZJU3）和蜡粉缺失突变体P1为试验材料，采用溶液培养法，待幼苗生长到2叶时，用不同浓度的聚乙二醇6000（PEG-6000）模拟干旱胁迫，比较大麦蜡粉缺失突变体和野生型幼苗的生理生化指标响应及10个蜡质相关基因的表达情况。结果表明：在干旱胁迫下，超氧化物歧化酶、过氧化物酶活性先上升后下降，脯氨酸含量持续上升，丙二醛含量上升，表明大麦蜡粉的缺失会降低其抗旱能力。选取的10个蜡质基因中，P1相对于ZJU3有6个基因表达下调，3个基因表达上调，1个基因表达未见明显差异。本研究初步揭示了蜡粉缺失突变体的特性及野生型较蜡粉缺失突变体在抗旱性上的优势。

关键词： 大麦, 干旱胁迫, 生理生化指标, 蜡质基因, 表达模式

Table 1 Primers of barley wax synthesis related genes

Fig. 1 Antioxidant enzyme activities of P1 and ZJU3 seedlings under drought stress

Fig. 2 Pro and MDA contents of P1 and ZJU3 seedlings under drought stress

Fig.3 Expression of waxy genes of mutant P1 and wild-type ZJU3 seedlings under drought stress


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