Using “Sanbenti”， “11-06-25”， “Zaoxia Wuhe” and “Summer Black” and four reference varieties including two Vitis vinifera L. cv. “Muscat of Red Alexandria” and “Shien” along with two Euro-American hybrids “Shine-Muscat” and “Kyoho” as materials, an analysis of their genetic relationship by SSR (simple sequence repeat) was carried out. 35 pairs of SSR primers were screened for PCR amplification. By these primers, a total of 135 robust and reproducible bands were generated, of which 124 were polymorphic and the polymorphism frequency was 91.85%. An unweighted pair-group method with arithmetic mean (UPGMA) dendrogram of eight genotypes using NTSYS-pc 2.10e was constructed.

　　Based on our results, it could be established that “Sanbenti”, “11-06-25”, “Zaoxia Wuhe” and “Summer Black” showed high similarity. According to the parents and bud sports relation, the genetic similarity coefficient between “Sanbenti” and “11-06-25” is the highest. Although “11-06-25” and “Zaoxia Wuhe” are similar in some biological traits, the DNA band patterns of “11-06-25” and “Zaoxia Wuhe” varied in four loci (VrZAG15, VrZAG64, VMC4A1 and VMC9A2.1), suggesting that they are not identical grape lines. Besides, during preliminary observation, “11-06-25” showed better color than that of “Sanbenti”, and it also showed better in fruit quality especially aroma than that of “Zaoxia Wuhe” under the same cultivation condition.

　　In conclusion,“11-06-25” is a new grape line derived from “Sanbenti”. It is very worth popularizing because of its early maturity time and great fruit quality. In addition, we presumed that “Sanbenti” may be a bud sport of “Summer Black”.