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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2019, Vol. 45 Issue (6): 736-745    DOI: 10.3785/j.issn.1008-9209.2019.04.041
Resource utilization & environmental protection     
Morphology, phylogeny and taxonomy of Amolops ricketti (Amphibia, Ranidae) in Guizhou Province
Ning XIAO1(),Qinghua LUO2,Huaiqing DENG2,Jun ZHOU2,Tao LUO3()
1.Guiyang Nursing Vocational College, Guiyang 550081, China
2.School of Life Sciences, Guizhou Normal University, Guiyang 550001, China
3.School of Karst Science, Guizhou Normal University, Guiyang 550001, China
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Abstract  

Fourteen specimens of Amolops ricketti collected in Guizhou Province were compared with the groups from A. ricketti, based on the analysis of morphology and mitochondrial 16S rRNA and COI sequence. The results showed that the different local populations of A. ricketti from Guizhou Province had the similar morphological characters with the species of A. sinensis, and the average genetic distances between the local populations of A. ricketti from Guizhou Province and A. sinensis (type locality: Shimentai Nature Reserve, Guangdong) were 0.74%-1.30%, and that of A. ricketti (type locality: Mt. Wuyi, Fujian) were 6.82%-6.96%. Meanwhile, a phylogenetic tree constructed based on the mitochondrial joint sequence showed that A. ricketti from Guizhou Province and A. sinensis (type locality: Shimentai Nature Reserve, Guangdong) were clustered a clade. Therefore, the original records of A. ricketti in the northern part (Xishui, Kuankuoshui), the central part (Huangping, Majiang), the eastern part (Jiangkou), the southeast part (Leigongshan, Danzhai) of Guizhou Province should be revised to A. sinensis. These origins represent the distribution of the previous records of A. ricketti in Guizhou, and it is proposed to amend the A. ricketti distributed in Guizhou Province to A. sinensis. It was indicated that the differentiation of A. sinensis local populations in the north, west, and south of the Wujiang River was related to the geographical barrier of Wujiang River system, and it should be further resolved.

Key words： Guizhou Province      taxonomic revision      Amolops sinensis      Amolops ricketti     
Received: 04 April 2019      Published: 20 January 2020
CLC:  Q 959.5  
Corresponding Authors: Tao LUO     E-mail: armiger@163.com;18786413778@163.com
About author: 罗庆华：共同第一作者
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Cite this article:

Ning XIAO,Qinghua LUO,Huaiqing DENG,Jun ZHOU,Tao LUO. Morphology, phylogeny and taxonomy of Amolops ricketti (Amphibia, Ranidae) in Guizhou Province. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(6): 736-745.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2019.04.041     OR     http://www.zjujournals.com/agr/Y2019/V45/I6/736


贵州分布的华南湍蛙形态、系统发育与分类

结合形态学和线粒体16SrRNA、COI序列分析，对贵州省原记载的华南湍蛙与华南湍蛙种组物种进行形态比对和系统发育分析，并基于线粒体联合序列数据和p-distances模型计算贵州种群与华南湍蛙种组物种之间的遗传距离。结果显示：贵州省所分布的华南湍蛙形态学特征与中华湍蛙相吻合；贵州分布的华南湍蛙与来自模式产地广东石门台分布的中华湍蛙的遗传距离为0.74%～1.30%，与模式产地福建武夷山分布的华南湍蛙的遗传距离为6.82%～6.96%；同时，基于线粒体联合序列构建的系统发育树显示，来自贵州的华南湍蛙与模式产地（广东石门台）中华湍蛙聚为一支。因此，贵州北部（习水、宽阔水）、中部（黄平、麻江）、东部（江口）、东南部（雷公山、丹寨）原记录的华南湍蛙应修订为中华湍蛙，这些分布地代表了贵州以往记录华南湍蛙的分布区域，进而建议将贵州喀斯特地区分布的华南湍蛙修订为中华湍蛙。乌江以北、以西和以南地区中华湍蛙地理种群的分化可能与乌江水系造成的地理阻隔有关，有待深入研究。


关键词： 贵州省,  分类修订,  中华湍蛙（Amolops sinensis）,  华南湍蛙（Amolops ricketti） 

序号

ID

种名

Species

采集地点

Locality

凭证

Voucher

GenBank登录号

GenBank accession No.

16S rRNACOI
1A. sinensis贵州省黄平县朱家山县级自然保护区GZNU2018052038MN640863MN643605
2贵州省黄平县朱家山县级自然保护区GZNU2018052041MN640864MN643606
3贵州省江口县梵净山国家级自然保护区GZNU201700515001MN640865MN643607
4贵州省江口县梵净山国家级自然保护区GZNU201700515002MN640866MN643608
5贵州省丹寨县排洞镇GZNU201805001MN640867MN643609
6贵州省雷山县雷公山国家级自然保护区GZNU201805002MN640868MN643610
7贵州省麻江县龙山乡GZNU201806001MN640869MN643611
8贵州省麻江县龙山乡GZNU201806002MN640870MN643612
9贵州省麻江县龙山乡GZNU201806003MN640871MN643613
10贵州省麻江县龙山乡GZNU201806004MN640872MN643614
11贵州省麻江县龙山乡GZNU201806005MN640873MN643615
12贵州省习水县习水国家级自然保护区GZNU20170815001MN640874MN643616
13贵州省习水县习水国家级自然保护区GZNU20170815002MN640875MN643617
14贵州省绥阳县宽阔水国家级自然保护区GZNU20170815003MN640876MN643618
15A. sinensis广东省石门台自然保护区*SYS a004165MK263262MK263314
16广东省石门台自然保护区*SYS a004722MK263278MK263318
17广东省石门台自然保护区*SYS a007105MK263297MK263329
18广东省石门台自然保护区*SYS a007106MK263298MK263330
19A. yatseni广东省中山市*SYS a006806MK263289MK263323
20广东省中山市*SYS a006807MK263290MK263324
21广东省中山市*SYS a006808MK263291MK263325
22A. albispinus广东省深圳市梧桐山*SYS a003452MK263247KX507332
23广东省深圳市梧桐山*SYS a003453MK263248KX507333
24广东省深圳市梧桐山*SYS a003454MK263249KX507334
25A. yunkaiensis广东省鹅凰嶂自然保护区*SYS a003979MK263253MG991907
26广东省鹅凰嶂自然保护区*SYS a004705MK263275MG991906
27广东省鹅凰嶂自然保护区*SYS a004706MK263276MG991908
28A. ricketti福建省武夷山*SYS a004141MK263259MG991927
29福建省武夷山*SYS a004142MK263260MG991928
30福建省武夷山*SYS a004143MK263261MG991929
31A. wuyiensis福建省武夷山*SYS a001717MK263240KX507325
32福建省武夷山*SYS a004139MK263257MK263312
33福建省武夷山*SYS a001716MK263239KX507324
34A. daiyunensis福建省戴云山*SYS a001737MK263241KX507326
35福建省戴云山*SYS a001738MK263242KX507327
36福建省戴云山*SYS a001739MK263243KX507328
37A. hongkongensis香港*SYS a004577MK263266MG991919
38香港*SYS a004578MK263267MG991920
39香港*SYS a004579MK263268MG991921
40A. hainanensis海南省五指山*SYS a005281MK263281MG991916
41海南省五指山*SYS a005283MK263283MG991918
42海南省五指山*SYS a005282MK263282MG991917
43A. torrentis海南省五指山*SYS a005289MK263284MG991930
44海南省五指山*SYS a005290MK263285MG991931
45海南省五指山*SYS a005291MK263286MG991932
46A. chunganensis江西省井冈山SYS a004212MK263263MG991914
47江西省井冈山SYS a004213MK263264MG991915
Table 1 Locality， voucher and GenBank accession No. for all samples used in this study
Fig. 1 Phylogenetic tree constructed by Bayesian inference and maximum likelihood methodPlease see the Table 1 for the details of each ID. The bootstrap supports (BS) for maximum likelihood analysis＞75 and the Bayesian posterior probabilities (BPP)＞0.85 were retained.

序号

No.

种名

Species

12345678910111213
1A. sinensis#
2A. sinensis*0.74
3A. sinensis**1.141.30
4A. yatseni3.783.343.61
5A. albispinus3.723.673.614.05
6A. yunkaiensis6.426.496.406.886.76
7A. ricketti6.826.866.966.496.698.03
8A. wuyiensis5.525.805.546.006.247.695.10
9A. daiyunensis12.0712.3312.0212.1112.1212.4112.6912.26
10A. hongkongensis11.8012.3311.4611.7211.8612.3411.9111.705.34
11A. hainanensis14.1315.2514.1215.7314.7216.1715.1114.3914.9515.89
12A. torrentis15.0915.6814.9515.5615.4516.4915.2215.3114.9914.6211.31
13A. chunganensis15.5615.7815.3515.5215.5415.2814.9414.5014.8515.5617.5216.81
Table 2 p-distances among the species of the genus Amolops in this study %

指标

Index

雄性 Male雌性 Female

范围

Range

平均值±标准差

Mean±standard deviation

范围

Range

平均值±标准差

Mean±standard deviation

体长 SVL41.76～60.7449.59±5.0344.96～60.2651.67±4.32
头长 HDL14.62～20.7717.51±1.4615.11～20.0217.71±1.35
头宽 HDW14.90～21.5117.73±1.8814.86～20.8217.33±1.41
吻长 SNT5.35～7.946.89±0.695.83～8.277.12±0.61
鼻间距 IND5.03～7.346.01±0.644.91～7.816.32±0.69
眼间距 IOD3.53～6.874.43±0.673.24～7.434.44±0.84
鼓膜到眼后角距离 TED1.47～3.252.34±0.391.64～3.482.53±0.48
眼径 ED3.43～5.985.23±0.543.84～7.385.60±0.68
鼓膜径 TD0.94～2.281.42±0.320.55～2.321.48±0.41
手长 HND11.85～16.1522.02±2.3410.93～15.5113.52±1.11
前臂及手长 LAL17.52～25.8422.02±2.3417.21～25.1421.25±2.01
足长 FTL19.78～29.1124.54±2.6319.88～29.0123.79±2.32
胫长 TIB20.79～31.3527.06±2.5021.42～30.6226.79±2.16
后肢全长 HLL69.22～91.8882.16±7.0868.34～99.0182.78±6.97
头长/体长 HDL/SVL0.31～0.410.36±0.020.29～0.400.34±0.02
头宽/体长 HDW/SVL0.32～0.420.35±0.020.30～0.360.33±0.02
头宽/头长 HDW/HDL0.92～1.141.00±0.060.82～1.150.98±0.06
吻长/头长 SNT/HDL0.30～0.440.39±0.030.34～0.480.40±0.04
吻长/体长 SNT/SVL0.11～0.160.14±0.010.12～0.160.14±0.01
鼻间距/头宽 IND/HDW0.27～0.430.34±0.030.30～0.430.37±0.04
眼间距/头宽 IOD/HDW0.19～0.350.25±0.030.19～0.410.26±0.05
眼径/头长 ED/HDL0.21～0.360.29±0.030.21～0.410.32±0.04
眼径/体长 ED/SVL0.07～0.140.11±0.010.07～0.130.11±0.01
鼓膜径/眼径 TD/ED0.18～0.500.27±0.070.11～0.420.27±0.08
鼓膜到眼后角距离/鼓膜径 TED/TD0.89～2.381.72±0.410.29～0.870.46±0.11
手长/体长 HND/SVL0.23～0.330.28±0.020.22～0.330.26±0.02
足长/体长 FTL/SVL0.40～0.550.49±0.030.39～0.570.46±0.04
胫长/体长 TIB/SVL0.47～0.600.55±0.040.40～0.630.52±0.05
Table 3 Measurements of the type series of A. sinensis from Guizhou Province mm
Fig. 2 Adult of A. sinensisA. Side view (♀); B. Dorsal view (♀); C. Side view (♂; the red arrow indicates a longitudinal glandular folds on skin of shoulder); D. Dorsal view (♂; the white arrow indicates pink nuptial spines).
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