Animal sciences & veterinary medicine |
|
|
|
|
Establishment and application of a polymerase chain reaction method for detection of Melissococcus pluton in honeybee (Apis cerana cerana) in Chun’an County of Zhejiang Province |
Rui GUO1(),Jie DONG1,Haikun GUO2,Junzhu LI3,Deqian WANG1() |
1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 3. Hao Qing-mi Beekeeping Professional Cooperative of Qiandao Lake of Zhejiang, Hangzhou 311701, China |
|
|
Abstract A polymerase chain reaction (PCR) method was developed to detect Melissococcus pluton and an epidemiological investigation of European foulbrood (EFB) was done in the main bee (Apis cerana cerana) breeding area of Chun’an County, Zhejiang Province. Results indicated that the newly developed method was specific, sensitive, repeatable and accuracy. The epidemiological survey results showed that a total of 11 townships were infected by M. pluton among the 298 bee samples in 17 townships of Chun’an County. The total infection rate was 25.2%, of which EFB infection rate of five townships was ≥40%. In a word, the newly established method for PCR detection makes the infection status of EFB clear in Chun’an County, and it lays a scientific basis for formulating rationally prevention measures of EFB.
|
Received: 18 July 2018
Published: 25 June 2019
|
|
Corresponding Authors:
Deqian WANG
E-mail: 754894816@qq.com;wangdeqian2002@sohu.com
|
浙江省淳安县中华蜜蜂蜂房球菌聚合酶链式反应检测方法的建立与应用
通过建立蜂房球菌(Melissococcus pluton)的聚合酶链式反应(polymerase chain reaction, PCR)检测方法,对浙江省淳安县主要中华蜜蜂(Apis cerana cerana)饲养区进行欧洲幼虫腐臭病的流行病学调查。结果显示,新建立的蜂房球菌PCR检测方法特异性强,灵敏度高,并具有良好的重复性和准确性。流行病学调查表明,在淳安县17个乡镇的298群蜜蜂样品中,共有11个乡镇检出蜂房球菌,检出率为25.2%,其中5个乡镇的阳性检出率≥40%。总之,本研究建立了一个新的蜂房球菌PCR检测方法,明确了淳安县中华蜜蜂对欧洲幼虫腐臭病的感染情况,为合理制定该病害的防治措施提供了科学依据。
关键词:
中华蜜蜂,
蜂房球菌,
欧洲幼虫腐臭病,
聚合酶链式反应,
淳安县,
流行病学
|
|
[1] |
刘学录,童金凤,马振刚 .蜜蜂主要病害及其病原PCR检测研究进展.南方农业学报,2016,47(1):147-152. LIU X L , TONG J F , MA Z G . Research progress on main bee diseases and PCR detection of pathogens. Journal of Southern Agriculture, 2016,47(1):147-152. (in Chinese with English abstract)
|
|
|
[2] |
张其安,王娟,杨少波 .蜜蜂细菌性疾病及其防治的研究进展.中国蜂业,2011,62:25-30. ZHANG Q A , WANG J , YANG S B . Research advances on honeybees bacterial diseases and treatment. Apiculture of China, 2011,62:25-30. (in Chinese with English abstract)
|
|
|
[3] |
卢焕仙,夏培康 .蜜蜂欧洲幼虫腐臭病的发生原因及防治措施.农村实用技术,2009(3):54-55. LU H X , XIA P K . Occurrence causes and control measures of European foulbrood. Rural Practical Technology, 2009(3):54-55. (in Chinese)
|
|
|
[4] |
BAILEY L , BALL B V . Honey Bee Pathology. 2nd ed. London, UK: Academic Press, 1991.
|
|
|
[5] |
GOVAN V A , ALLSOPP M H , DAVISON S . A PCR detection method for rapid identification of Paenibacillus larvae . Applied Environmental Microbiology, 1999,65(5):2243-2245.
|
|
|
[6] |
周婷,冯峰,董秉义 .中华蜜蜂的欧洲幼虫腐臭病病原研究.昆虫学报,2000,43(增刊1):104-108. ZHOU T , FENG F , DONG B Y . Study on the pathogen of European larvae rot disease of Chinese honeybee. Acta Entomologica Sinica, 2000,43(Suppl. 1):104-108. (in Chinese)
|
|
|
[7] |
HORNITZKY M A Z , WILSON S C . A system for the diagnosis of the major bacterial brood diseases of honeybees. Journal of Apicultural Research, 1989,28(4):191-195.
|
|
|
[8] |
OMAR R E M , Al E . Isolation and identification of American and European foulbrood from honeybee Apis mellifera (L.). Bulletin of the Entomological Society of Egypt, 2007,85(1):243-256.
|
|
|
[9] |
PINNOCK D E , FEATHERSTONE N E . Detection and quantification of Melissococcus pluton infection in honeybee colonies by means of enzyme-linked immunosorbent assay. Journal of Apicultural Research, 2015,23(3):168-170.
|
|
|
[10] |
CAPINERA J L . Encyclopedia of Entomology. Dordrecht, the Netherlands: Springer, 2008:1377.
|
|
|
[11] |
汪志铮 .蜜蜂欧洲幼虫腐臭病的防治.贵州畜牧兽医,2017,41(3):59. WANG Z Z . Prevention and control of European foulbrood in honeybee. Guizhou Journal of Animal Husbandry & Veterinary Medicine, 2017,41(3):59. (in Chinese)
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|