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| Effects of chilled trash fish and different carbohydrate levels in puffed feed on growth performance and glucose metabolism of snakehead |
| HOU Yong1, HOU Yanbin1, YAO Lei1, DAI Binyang1, QIAN Lichun1*, FAN Jinghui2* |
1. Key Laboratory of Animal Nutrition and Feed Science in East China of the Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; 2. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
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Abstract
A feeding experiment with different diets was conducted on snakehead (Ophiocephalus argus). Experimental groupⅠ was fed with chilled trash fish, and the other experimental groups were fed with puffed feed, of which group Ⅱ, Ⅲ and Ⅳ were added 13%, 16% and 19% flour with carbohydrate content of 17.48%, 19.55% and 21.63%, respectively. The effects of chilled trash fish and different levels of carbohydrate diets on growth performance, plasma glucose content, key enzymes of glucose metabolism and relevant hormone variations of snakehead were investigated at different time after feeding. The results showed that compared with the experimental groupⅠ, the mass gain rate and specific growth rate of all the other experimental groups decreased significantly (P＜0.05), as well as the feed efficiency and feeding rate (P＜0.05). The plasma glucose level of snakehead fed with puffed feed was significantly higher than that of the snakehead fed with chilled trash fish (P＜0.05), and reached the maximum at 9 h after feeding. With the increase of carbohydrate level in puffed feed, the content of hepatic glycogen had an increase trend, and the hepatopancreas index also significantly increased (P＜0.05); moreover, the activities of hexokinase (HK) and glucokinase (GK) in group Ⅱ, Ⅲ and Ⅳ increased significantly than those in groupⅠ(P＜0.05), but the difference of fructose 1, 6 bisphosphatase activity in the treatment groups was not significant (P＞0.05). After feeding 3, 6 and 12 h, compared with the group Ⅰ, the content of insulin in the snakehead fed with puffed feed increased, and in particular the group Ⅱ and Ⅳ increased significantly (P＜0.05); while after feeding 9 and 24 h, the content of insulin in the snakehead of group Ⅳ significantly decreased than that in the group Ⅰ (P＜0.05). In the early period (3, 6 h) after feeding, as carbohydrate levels increased in puffed feed, the content of plasma glucagon in the snakehead of experimental groups significantly increased (P＜0.05) than that in the group Ⅰ, while the level of plasma glucagon after feeding 9 h significantly lowered (P＜0.05).
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Published: 27 April 2018
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冰鲜鱼和膨化饲料中不同糖类水平对乌鳢生长性能及糖代谢的影响
以乌鳢（Ophiocephalus argus）为研究对象，采用不同日粮饲喂，试验Ⅰ组饲喂冰鲜鱼，其他试验组饲喂膨化饲料，其中试验Ⅱ组、Ⅲ组和Ⅳ组面粉添加量分别为13%、16%和19%（含糖量分别为17.48%、19.55%和21.63%），研究冰鲜鱼和膨化饲料中不同糖类水平对乌鳢生长性能、血糖、糖代谢关键酶活性及相关激素的影响。结果表明：与试验Ⅰ组相比，其他各试验组的增质量率和特定生长率均显著下降（P＜0.05），饲料效率与摄食率也显著降低（P＜0.05）。乌鳢采食膨化饲料后的血糖水平显著高于冰鲜鱼组（P＜0.05），且都在摄食后9 h 达到最大值。各试验组随着膨化饲料中糖类水平的升高，肝糖原含量呈升高趋势，同时肝胰腺指数也显著升高（P＜0.05）；此外，试验Ⅱ、Ⅲ和Ⅳ组己糖激酶（hexokinase, HK）活性和葡萄糖激酶（glucokinase, GK）活性与试验Ⅰ组相比较均显著升高（P＜0.05），但果糖1，6-二磷酸酶（fructose 1, 6-bisphosphatase, FBPase）的活性差异均不显著（P＞0.05）。乌鳢摄食3、6和12 h 后，与没有采食膨化饲料的试验Ⅰ组相比，采食膨化饲料的乌鳢血浆中胰岛素含量上升，且试验Ⅱ和Ⅳ组显著升高（P＜0.05）；乌鳢摄食9 和24 h 后，与试验Ⅰ组相比，试验Ⅳ组血浆胰岛素含量显著降低（P＜0.05）。摄食后前期（3 和6 h），随着膨化饲料中糖类含量的上升，采食膨化饲料的乌鳢血浆胰高血糖素含量与采食冰鲜鱼的乌鳢相比显著升高（P＜0.05），而摄食后9 h，其血浆胰高血糖素含量显著降低（P＜0.05）。
关键词：
乌鳢,
糖类,
生长性能,
糖代谢关键酶,
激素
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