| Biological sciences & biotechnology |
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| Statistical analysis of carbon and nitrogen sources for growth and sporulation of Exserohilum rostratum causing banana leaf spot disease |
| Du Ruiqing1, Lin Shanhai2,3,Huang Siliang1*, Zhang Zhengtian1, Qin Liping4, Li Qiqin3 |
| (1. School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, Henan, China; 2. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3. College of Agriculture, Guangxi University, Nanning 530005, China; 4. Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China) |
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Abstract Exserohilum rostratum is one of the important pathogens causing banana leaf spots, which has a wide host range. The effects of carbon and nitrogen sources on colony diameters and sporulation among different isolates of E. rostratum have been analyzed using conventional variance analysis method; however, this method was impossible to differentiate the common carbon and nitrogen sources from the species and the isolate-specific ones for growth and sporulation. In-depth difference analyses of various carbon and nitrogen sources were performed to determine the nutritional characteristics of E. rostratum causing banana leaf spot disease, and to provide a basis for disease management. Three isolates (CLER09, D087 and JL05) of the pathogen were used as the experimental ones. The Czapeks medium was used as a basal medium for nutritional tests on carbon and nitrogen sources. The sucrose in the basal medium was substituted with an equal amount of each of the 20 carbon sources tested. The potassium nitrate in the basal medium was substituted with an equal amount of each of the 26 nitrogen sources tested. The basal medium lacking sucrose and that lacking potassium nitrate were used as the controls for carbon and nitrogen utilization tests, respectively. The three isolates were inoculated on the basal media containing different carbon and nitrogen sources at 28 ℃ for 4 days. The colony diameters and the numbers of conidia produced were separately investigated. The data obtained were evaluated using multiple statistical methods including cluster analysis, discriminatory analysis and comprehensive correlation analysis. The results indicated that the carbon sources had significant effect on growth and sporulation of isolates CLER09, D087 and JL05. Of the carbon sources tested, lactose was identified as the most suitable general carbon source for growth and sporulation of the three isolates. Maltose, sucrose, glucose, α-lactose, xylitol, D-mannose, D-galactose, soluble starch, xylose, L-arabinose, inositol, dextrin and glycerin were identified as the suitable carbon sources for growth and sporulation. Significant correlation was observed between the mean colony diameters and the numbers of conidia produced among the three isolates. Nitrogen sources had significant effects on the numbers of conidia produced, not on the colony diameters. L-cysteine and L-phenylalanine were the most suitable nitrogen sources for growth and sporulation of the three isolates. L-proline and potassium nitrate were identified as the suitable nitrogen sources for growth and sporulation of the three isolates. The isolate CLER09 had the following nutritional characteristics: maltose, α-lactose, D-mannose and dextrin as the carbon sources were suitable for growth and sporulation followed by L-histidine; sucrose as a carbon source was unsuitable for growth and sporulation; significantly positive correlation was observed between the colony diameters and the numbers of conidia produced with reference to the carbon sources; no significantly positive correlation was observed between the colony diameters and the numbers of conidia produced with reference to the nitrogen sources. The isolate D087 had the following nutritional characteristics: D-mannose as a carbon source was unsuitable for growth and sporulation; asparagines, thymine, glutamic acid and vitamin B1 had the secondary suitability as the carbon sources for growth and sporulation; significantly positive correlation was observed between the colony diameters and the numbers of conidia produced with reference to the carbon sources; no significantly positive correlation was observed between the colony diameters and the numbers of conidia produced with reference to the nitrogen sources. Isolate JL05 had the following nutritional characteristics: glucose and trehalose were the most suitable carbon sources for growth and sporulation followed by mannitol; no significantly positive correlation was observed between the colony diameters and the numbers of conidia produced with reference to both carbon and nitrogen sources. In conclusion, general and individual carbon and nitrogen requirements existed among isolates of E. rostratum for growth and sporulation.
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Published: 20 January 2015
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对影响香蕉叶斑病菌喙突脐蠕孢生长和产孢碳、氮源的统计学分析
为了解不同碳源和氮源对香蕉叶斑病菌喙突脐蠕孢不同菌株的生长和产孢影响存在的共性与个性差异,该文利用聚类分析、判别分析和相关分析等多种统计分析法对实验数据进行深度解析。分析结果显示,碳源对菌株CLER09、D087与JL05的生长和产孢量有显著影响,其中乳糖是供试3菌株最适生长和产孢的碳源;在不同碳源中供试3菌株的菌落直径和产孢量之间呈极显著相关。氮源对菌株CLER09、D087与JL05的产孢量有显著影响,对菌落直径无显著影响,其中L-半胱氨酸与L-苯丙氨酸是供试3菌株生长和产孢的最适宜氮源。同时,各菌株对碳源和氮源又表现出各自的特性。因此,对于适宜香蕉叶斑病菌喙突脐蠕孢生长和产孢的碳源和氮源的选取,多菌株主要考虑其共性碳源和氮源,单菌株则应考虑菌株的各自特性碳源和氮源。
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