| Biological sciences & biotechnology |
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| Relationship between IGF2a intron 3 single nucleotide polymorphisms and growth performance of Cyprinus carpio |
| Su Shengyan1, Dong Zaijie1,2*, Zhu Wenbin1, Yuan Xinhua1 |
(1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China; 2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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Abstract (SNP) and growth traits correlation between FFRC strain carp, intron 3 SNP of IGF2a gene about C. carpio var. FFRC strain carp as experimental materials. When they were grown up to 10 g, all fish were tagged by passive integrated transponder (
PIT) approach. Then the same environment was created to rear such fish for five months using commercial feed. A total of 32 carp were selected randomly and measured to have the growth performance records from the concrete tanks in Yixing, which are affiliated to the Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences. These fish were slaughtered and immediately dissected to collect 50100 mg breast muscle. After the DNA extraction and PCR amplification, using TaKaRa kits, the SNP and the relationships between such locus and C. carpio var. FFRC strain carp were analyzed by sequence and bioinformatics’ software. The relationships between SNP detected and FFRC strain carp growth traits were also analyzed by SAS 8.0. In the present study, DNA sequence (468 bp) of common carp IGF2a intron 3 was isolated and 4 SNPs in common carp IGF2a were found and named by their location. Of them, heterozygous A/G genotype located 256257 could reduce the body mass and body length of FFRC strain carp; nevertheless, the mass reduction rate is higher than the body length, however both body mass and body length reduction was not significant. The body length and body mass ratio for
heterozygous A/G genotype and homozygous G/G genotype had remarkable difference; meanwhile, the heterozygote makes the correlation coefficient of body length and body mass increase. The result also showed that female’s body mass, body length and the ratio of body mass to body length were higher than males for G/G genotype, but the ratio of body length to body mass was larger, and the absolute values of body mass and body length were smaller and the ratio of body length to body mass had significant difference between the male and female fish. Generally, the above results suggest that the IGF2a intron 3 polymorphism can influence the body mass and body length of
FFRC strain carp. Moreover, heterozygous A/G genotype located 256257 may be related to visceral mass gain owing to the remarkable effect on body mass and body length ratio. Furthermore, such locus can make interaction with gender by influencing the growth performance of carp. These results will provide information for molecular assisted breeding by increasing the IGF2a heterozygote percentage of the whole population.
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Published: 20 September 2014
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鲤IGF2a基因内含子3的单核苷酸多态性与福瑞鲤生长性状的相关性
为了解鲤IGF2a基因单核苷酸多态性（single nucleotide polymorphism,SNP）与福瑞鲤(FFRC strain carp)生长性状的相关性,以福瑞鲤为实验材料,设计用于分析IGF2a基因内含子3的SNP引物,分析其SNP,再结合福瑞鲤的生长性状,探讨两者之间的相关性。研究发现:杂合子A/G基因型使福瑞鲤体质量、体长2个生长指标减小,体质量的降低幅度高于体长,但是两者差异均无统计学意义（P>0.05）;而杂合子A/G基因型和纯合子G/G基因型的体长与体质量的比值差异有统计学意义（P<0.05）。这表明IGF2a基因可影响福瑞鲤的体质量、体长,且其SNP可能与福瑞鲤内脏质量增加有关。同时,研究发现这个多态位点与性别在对鲤的生长性能的影响上存在互作。这些结果将为基于内含子3的IGF2a基因分子辅助育种奠定基础。
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