Please wait a minute...
浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2013, Vol. 39 Issue (6): 645-654    DOI: 10.3785/j.issn.1008-9209.2013.04.071
Agricultural sciences     
Comparison of leaf epidermis and pollen micromorphology among Dimocarpus longana and Litchi chinensis cultivars in Western Guangdong
LIU Kaidong, YUAN Changchun*, CHEN Yan, XIAO Zhengquan, LIU Zhu
(Life Science and Technology School, Zhanjiang Normal University, Zhanjiang, Guangdong 524048, China)
Download: HTML ( )    PDF(11719KB)
Export: BibTeX | EndNote (RIS)      

Abstract  Longan (Dimocarpus longana Lour.) and litchi (Litchi chinensis Sonn.) are famous and special fruits in tropical and subtropical zones. Longan and litchi originate in China, which have been cultivated for more than 2 000 years and both hold abundant germplasm resources. There have been many scientific researches on longan and litchi. However, few systematic studies were conducted on comparison of leaf epidermis and pollen grains between longan and litchi. Therefore, the aim of this study is to compare the differences of leaf epidermis and pollen micromorphology among longan and litchi cultivars, and then to study their phylogenetic relationship. The ultrastructures of leaf epidermis and pollen grains from six longan and six litchi cultivars in Western Guangdong Province were examined using scanning electron microscope, and their relationship with the phylogenetic evolution of the genuses was investigated. The results showed that numerous protuberances could be observed on the lower epidermis. The protuberances of litchi were domeshaped, and those of longan were longshaped. There were significant differences in the stomatal densities and sizes of longan and litchi cultivars. The stomatal densities of six longan cultivars were 316.30406.00 No./mm2, while those of six litchi cultivars were 519.00655.20 No./mm2. The stomatal lengths of six longan cultivars were 11.1621.33 μm, while those of six litchi cultivars were 10.3817.28 μm. The pollen grains of longan and litchi were tricolporate, belonged to N3P4C5 type according to Erdtman’s NPC system. These pollen grains were subspheroidal or wide ellipse in equatorial view, and 3lobed subspheroidal or triangle in polar view. The polar axis lengths of pollen grains in six longan cultivars were 18.3119.85 μm, while those in six litchi cultivars were 16.1918.07 μm. The equatorial axis lengths of pollen grains in six longan cultivars was 21.3624.33 μm, while those in six litchi cultivars were 19.2221.47 μm. In addition, there were significant differences in some morphological aspects of pollen grains among longan and litchi cultivars, such as pollen size and colporate. The characteristic differences of leaf epidermis and pollen grains were obvious among longan and litchi cultivars. Thus, it is suggested that the micromorphological characters of leaf epidermis and pollen grains are very important for cultivar differentiation in longan and litchi cultivars. Meanwhile, they can also provide valuable information for the study of taxonomy and phylogenesis among D. longana and L. chinensis cultivars.

Published: 20 November 2013
CLC:  S 667  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
LIU Kaidong
YUAN Changchun*
CHEN Yan
LIU Zhu
XIAO Zhengquan
Cite this article:

LIU Kaidong, YUAN Changchun*, CHEN Yan, XIAO Zhengquan, LIU Zhu. Comparison of leaf epidermis and pollen micromorphology among Dimocarpus longana and Litchi chinensis cultivars in Western Guangdong. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(6): 645-654.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2013.04.071     OR     http://www.zjujournals.com/agr/Y2013/V39/I6/645


粤西龙眼与荔枝叶表皮和花粉微形态的比较

为了解粤西地区龙眼与荔枝叶表皮和花粉微形态特征及其演化关系，利用扫描电子显微镜对粤西地区6个龙眼品种和6个荔枝品种的叶表皮及其花粉微形态进行观察.结果表明：龙眼、荔枝叶片下表皮有大量的乳状突，龙眼的乳状突为长形，荔枝的乳状突近半圆形；龙眼和荔枝不同品种叶片的下表皮气孔密度和气孔大小存在明显的差异；龙眼、荔枝品种的花粉都具有3条萌发沟，属N3P4C5类型；供试品种的赤道面观大都为圆形至宽椭圆形，极面观呈三角形和三裂圆形；龙眼、荔枝供试品种间的花粉大小和表面纹饰等均有明显差别.说明龙眼和荔枝的叶表皮和花粉微形态特征可作为品种间分类的一个重要参考.
No related articles found!