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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (12): 1013-1022    DOI: 10.1631/jzus.B1400163
Articles     
Zirconium oxide ceramic foam: a promising supporting biomaterial for massive production of glial cell line-derived neurotrophic factor
Zhong-wei Liu, Wen-qiang Li, Jun-kui Wang, Xian-cang Ma, Chen Liang, Peng Liu, Zheng Chu, Yong-hui Dang
Department of Cardiology, the Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710068, China; School of Astronautics, Northwestern Polytechnic University, Xi'an 710072, China; Department of Psychiatry, the First Affiliated Hospital of Xi\'an Jiaotong University, Xi'an 710061, China; Department of Neurosurgery, the First Affiliated Hospital of Xi\'an Jiaotong University, Xi'an 710061, China; College of Medicine & Forensics, Xi\'an Jiaotong University Health Science Center, Xi'an 710061, China; Key Laboratory of the Health Ministry for Forensic Medicine, Health Science Center, Xi\'an Jiaotong University, Xi'an 710061, China; MOE Key Laboratory of Environment and Genes Related to Diseases, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China
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Abstract  This study investigated the potential application of a zirconium oxide (ZrO2) ceramic foam culturing system to the production of glial cell line-derived neurotrophic factor (GDNF). Three sets of ZrO2 ceramic foams with different pore densities of 10, 20, and 30 pores per linear inch (PPI) were prepared to support a 3D culturing system. After primary astrocytes were cultured in these systems, production yields of GDNF were evaluated. The biomaterial biocompatibility, cell proliferation and activation of cellular signaling pathways in GDNF synthesis and secretion in the culturing systems were also assessed and compared with a conventional culturing system. In this study, we found that the ZrO2 ceramic foam culturing system was biocompatible, using which the GDNF yields were elevated and sustained by stimulated cell proliferation and activation of signaling pathways in astrocytes cultured in the system. In conclusion, the ZrO2 ceramic foam is promising for the development of a GDNF mass production device for Parkinson’s disease treatment.

Key words： Zirconium oxide      Ceramic foam      Glial cell line-derived neurotrophic factor (GDNF)      Parkinson’s disease     
Received: 12 June 2014      Published: 03 December 2014
CLC:  Q819  
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Zhong-wei Liu
Wen-qiang Li
Jun-kui Wang
Xian-cang Ma
Chen Liang
Peng Liu
Zheng Chu
Yong-hui Dang
Cite this article:

Zhong-wei Liu, Wen-qiang Li, Jun-kui Wang, Xian-cang Ma, Chen Liang, Peng Liu, Zheng Chu, Yong-hui Dang. Zirconium oxide ceramic foam: a promising supporting biomaterial for massive production of glial cell line-derived neurotrophic factor. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(12): 1013-1022.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400163     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I12/1013

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