| Biomedicine |
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| RNA interference against interleukin-5 attenuates airway inflammation and hyperresponsiveness in an asthma model |
| Shao-xing CHEN, Feng-ying HUANG, Guang-hong TAN, Cai-chun WANG, Yong-hao HUANG, Hua WANG, Song-lin ZHOU, Fan CHEN, Ying-ying LIN, Jun-bao LIU |
| Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China; Department of Digestive Diseases, Affiliated Hospital of Hainan Medical College, Haikou 570102, China |
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Abstract Interleukin-5 (IL-5) accompanies the development of airway inflammation and hyperresponsiveness through the activation of eosinophils. Therefore, interference of IL-5 expression in lung tissue seems to be an accepted approach in asthma therapy. In this study, we designed a small interfering RNA (siRNA) to inhibit the expression of IL-5. The siRNAs against IL-5 were constructed in a lentivirus expressing system, and 1.5×106 IFU (inclusion-forming unit) lentiviruses were administered intratracheally to ovalbumin (OVA)-sensitized murine asthmatic models. Our results show that lentivirus-delivered siRNA against IL-5 efficiently inhibited the IL-5 messenger ribonucleic acid (mRNA) expression and significantly attenuated the inflammation in lung tissue. Significant decrease of eosinophils and inflammatory cells were found in peripheral blood, bronchoalveolar lavage fluid (BALF), and lung tissue. In addition, significant inhibition of airway hyperresponsiveness (AHR) was found in the mice treated with siRNA against IL-5. These observations demonstrate that siRNA delivered by means of the lentivirus system is possibly an efficacious therapeutic approach for asthma.
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Received: 22 July 2008
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Cite this article:
Shao-xing CHEN, Feng-ying HUANG, Guang-hong TAN, Cai-chun WANG, Yong-hao HUANG, Hua WANG, Song-lin ZHOU, Fan CHEN, Ying-ying LIN, Jun-bao LIU. RNA interference against interleukin-5 attenuates airway inflammation and hyperresponsiveness in an asthma model. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(1): 22-28.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820226 OR http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I1/22
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