Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2005, Vol. 6 Issue (6): 454-456    DOI: 10.1631/jzus.2005.B0454
Biotechnology     
Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii
GAO Yong-gui, YAO Min, TANAKA Isao
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan; School of Life Sciences, Zhejiang University, Hangzhou 310029, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  RNA methyltransferase is responsible for transferring methyl and resulting in methylation on the bases or ribose ring of RNA, which existed widely but mostly remains an open question. A recombinant protein PH1948 predicting RNA methyltransferase from Pyrococcus horikoshii OT3 has been crystallized. The crystals of selenomethionyl PH1948 belong to space group C2, with unit-cell parameters a=207.0 Å, b=43.1 Å, c=118.2 Å, β=92.1°, and diffract X-rays to 2.2 Å resolution. The VM value was determined to be 2.8 Å3/Da, indicating the presence of four protein molecules in the asymmetric unit.

Key words： Pyrococcus horikoshii      Methyltransferase      X-ray diffraction      Protein crystallography     
Received: 25 March 2005     
CLC:  Q5  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
GAO Yong-gui
YAO Min
TANAKA Isao
Cite this article:

GAO Yong-gui, YAO Min, TANAKA Isao. Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(6): 454-456.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2005.B0454     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2005/V6/I6/454

[1] Yue Zhang, Hai-peng Lv, Cheng-ying Ma, Li Guo, Jun-feng Tan, Qun-hua Peng, Zhi Lin. Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(2): 103-112.
[2] Jun ZHANG, Xin-you XIE, Su-wen YANG, Jin WANG, Chao HE. Nicotinamide N-methyltransferase protein expression in renal cell cancer[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(2): 136-143.