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ZJUS-A
Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering)  2008, Vol. 9 Issue (7): 1004-1010    DOI: 10.1631/jzus.A0820064
Materials Science and Chemical Engineering     
Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis
Jian-fang WANG, Qing-liang ZHAO, Wen-biao JIN, Shi-jie YOU, Jin-na ZHANG
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; Department of Urban and Civil Engineering, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, China
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Abstract  Performance of biological phosphorus removal in the oxic-settling-anaerobic (OSA) process was investigated. Cell staining and fluorescent in situ hybridization (FISH) were used to analyze characteristics and microbial community of sludge. Experimental results showed that phosphorus removal efficiency was near 60% and the amount of biological phosphorus accumulation in aerobic sludge of the OSA system was up to 26.9 mg/g. Biological phosphorus removal efficiency was partially inhibited by carbon sources in the continuous OSA system. Contrasted to the OSA system, biological phosphorus removal efficiency was enhanced by 14% and the average total phosphorus (TP) contents of aerobic sludge were increased by 0.36 mg/g when sufficient carbon sources were supplied in batch experiments. Staining methods indicated that about 35% of microorganisms had typical characteristics of phosphorus accumulating organisms (PAOs). FISH analysis demonstrated that PAOMIX-binding bacteria were predominant microbial communities in the OSA system, which accounted for around 28% of total bacteria.

Key words： Excess sludge reduction      Biological phosphorus removal      Phosphate accumulating organisms (PAOs)      DAPI (4′,6′-diamidino-2-phenyl indol dihydrochloride)      Fluorescent in situ hybridization (FISH)     
Received: 02 January 2008      Published: 29 May 2008
CLC:  X703.1  
  DQ89  
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Jian-fang WANG
Qing-liang ZHAO
Wen-biao JIN
Shi-jie YOU
Jin-na ZHANG
Cite this article:

Jian-fang WANG, Qing-liang ZHAO, Wen-biao JIN, Shi-jie YOU, Jin-na ZHANG. Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis. Journal of Zhejiang University-SCIENCE A (Applied Physics & Engineering), 2008, 9(7): 1004-1010.

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http://www.zjujournals.com/xueshu/zjus-a/10.1631/jzus.A0820064     OR     http://www.zjujournals.com/xueshu/zjus-a/Y2008/V9/I7/1004

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