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Preparation of Monoclonal Antibody Against Neisseria Gonorrhoeae and Studies on Its Biological Characteristics
Shen Jiangen, Bao Jianfang, Shao Chuansen
Journal of ZheJiang University(Medical Science), 1999, 28(2): 49-50.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.001
Abstract( 147 )   HTML (   PDF(184KB)( 80 )

Objective:To develop monoclonal antibodies against Neisseria gonorrhoeae for establishing a new diagnostic method of gonorrhoea.Methods:Monoclonal antibodies against strain 29106-5 of N.gonorrhoeae were prepared by the lymphocyte hybridoma technique.The specificity and sensitivity of McAbs were studied using the sandwich method of ELISA with double antibodies.Results:Five cell lines of hybridoma secreting constantly McAbs to N.gonorrhoeae were obtained.The titer of McAbs reached to 1:8192~1:32786.All McAbs could react to N.gonorrhoeae,but failed to react to other microbe such as N.Meningitidis,staphylococcus and E.Coli.The minimum dose of N.gonorrhoeae which can be detected by ELISA was 1×104 bacteria per ml.Conclusion:The sandwich method of ELISA using McAbs to detect N.gonorrhoeae possessed higher specificity and sensitivity.

Study on the Formation of Metabolic Intermediate Complex of p-aminobenzoates with Cytochrome P450 using Molecular Orbital Method
Fu Xuchun, Jiang Huidi, Zhang Qin
Journal of ZheJiang University(Medical Science), 1999, 28(2): 51-53.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.002
Abstract( 42 )   HTML (   PDF(193KB)( 32 )

Objective:To study the correlations between the initial formation velocities (v) of the cytochrome P450 (P450 ) metabolic intermediate complex of p-aminobenzoates with rat hepatic microsomes and their molecular orbital indexes.Method:The semiempirical self consistent field molecular orbital calculation MNDO-PM3 method was utilized to obtain the molecular orbital indexes of the p-aminobenzoates investigated.Stepwise multiple regression analyses were then utilized to generate correlative equations between log v and the molecular orbital indexes.Results:There were no obvious correlations between log v and the energy of the highest occupied molecular orbital,the energy of the lowest unoccupied molecular orbital,the frontier electron density or the net charge of amino-nitrogen atom.Log v was well correlated parabolically with total molecular energy (ET),the electrophilic superdelocalizabilities summed over all atoms (∑SE) and the sum of the absolute values of the atomic charges (∑Q).Conclusion:The molecular orbital indexes including ET,∑SE and ∑Q can be used to predict the initial velocities (v) of the formation of the cytochrome P450 metabolic intermediate complex of p-aminobenzoates with rat hepatic microsomes.

Study of Transdermal Iontophoresis of Diclofenac Sodium Gelatin
Gao Jianqing, Liang Wenquan, Feng Xuan
Journal of ZheJiang University(Medical Science), 1999, 28(2): 54-56.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.003
Abstract( 52 )   HTML (   PDF(189KB)( 36 )

Objective:To select the satisfactory formula of diclofenac sodium gel.Methods:Four formulae of gelatin were prepared and the modified Franz difussion cells were employed in the transdermal iontophoretic experiments of these gelatins.Results:The fluxes of these four formulae were 63.4±8.02、56.0±5.82、61.2±6.14 and 60.8±5.25 μg/cm2/h respectively.There were no significant differences among them.The resistence of gelatin was reduced significantly after the gelatin absorbed the drug solution in 10 minutes.The study also demonstrated that if the adsorbent was added to the gelatin,there were no marked pH changes in gelatin before and after iontophoretic experiment.Conclusion:Formula Ⅳ is the best of all gelatins selected which has a good appearance and flexibility,and this gel can reduce the change of pH value caused by the electrical field.

HLA Class Ⅱ Antigens Matching in Cadaver Kidney Transplantation
Yan Lixing, Fu Qihua, Zhu Jun
Journal of ZheJiang University(Medical Science), 1999, 28(2): 57-58.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.004
Abstract( 46 )   HTML (   PDF(151KB)( 31 )

Objective:To study the possibility and necessity of HLA-class Ⅱ antigens matching in cadaver kidney transplantation.Methods:Using genotyping method,we typed HLA-DRB and DQB of 27 cadaver donors and 49 recipients of kidney transplantations retrospectively.Results:In those kidney transplantation donors and recipients who didn't do HLA matching test,fully-matched rate of HLA DRB and DQB were only 2.0% and 10.2% respectively;fully-not-matched rate were 30.6% and 8.2%.If the HLA were typed before transplantation then fully matched rate of HLA-DRB and DQB could reach 8.2% and 40.8% respectively in those donors and recipients;fully-not-matched rate was reduced to 10.2% and 0 respectively.The outcome of transplantation in fully not matched patients was worse than that in others.Conclusion:HLA-class Ⅱ antigens matching in cadaver kidney transplantation is not only possible,but also necessary.

The Ultrastructural Observation of Three Dimensional Culture of Fibroblasts
Han Chunmao
Journal of ZheJiang University(Medical Science), 1999, 28(2): 59-61.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.005
Abstract( 45 )   HTML (   PDF(161KB)( 29 )

Objective:To develop a three dimensional fibroblast culture method for wound healing studies.Methods:By using the permeable collagen membrane as a substrate,we modified the three-dimensional fibroblast culture.After being cultured for 6 weeks,the fibroblast cultures were double colored with uranium acetate and lead nitrate and finally observed with electron microscopy.Results: Besides the multilayer,dense extracellular matrix and myofibroblasts in the cultrue,myofibroblasts were also found in the lower layer of the culture.Conclusion:The fibroblast culture model can be used in the study of wound healing,especially of the hypertrophic scar and keloid.

Relationship between Plasma Levels of TXA2、PGI2 and Pulmonary Artery Hypertension in Congenital Ventricular Septal Defect
Lin Youmin, Kang Manli
Journal of ZheJiang University(Medical Science), 1999, 28(2): 62-64.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.006
Abstract( 58 )   HTML (   PDF(154KB)( 30 )

Objective:To investigate the relationship between plasma levels of TXA2、PGI2 and pulmonary artery hypertension in children with congenital ventricular septal defect (VSD).Method: The pulmonary pressure of 28 cases with congenital VSD was determined by catheterization or directly determined before surgery and the plasma levels of TXB2、6-keto-PGF (the metabolites of the TXA2、PGI2) were determined by the RIA.Ten healthy children were used as the control group.Results:①Compared with the control group,in normal pulmonary artery pressure group (NPAP),the plasma levels of TXB2、TXB2/6 keto PGF showed no significant change,but the level of 6-keto-PGF was significantly lower;in pulmonary artery hypertension group (PAH),the plasma levels of TXB2 and TXB2/6-keto-PGF were significantly increased,but the level of 6-keto-PGF was nearly normal.②Compared to the NPAP,the plasma levels of TXB2,TXB2/6-keto-PGF of the PAH were significantly increased,while the level of 6-keto-PGF was increased,but without statistical significance.Conclusion:The imbalance between plasma levels of TXA2、PGI2 may contribute to the course of pulmonary artery hypertension.

Analysis of Serum TNF-α and sTNFR-Ⅱ Levels in Neonatal Sepsis
Zheng Jiyan, Ma Ming, Chen Liqing
Journal of ZheJiang University(Medical Science), 1999, 28(2): 65-66.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.007
Abstract( 161 )   HTML (   PDF(110KB)( 57 )

Objective:To evaluate the significance of TNF-α,sTNFR-Ⅱ levels in diagnosis of neonatal sepsis.Method:The serum TNF-α and sTNFR-Ⅱ levels of 42 cases with neonatal sepsis and 20 non-infected newborn were measured with ELISA.Results:Serum levels of TNF-α and sTNFR-Ⅱ in septic patients were significantly higher than those of controls (P<0.001).Seurm TNF-α was positively correlated with sTNFR-Ⅱ.Conclusion:Serum TNF-α and sTNFR-Ⅱ increase in neonatal sepsis may indicate a good outcome for septic patients.

The Individual and Comprehensive Management in Acute Pancreatitis
Zheng Shusen, Liang Tingbo, Yu Jieren
Journal of ZheJiang University(Medical Science), 1999, 28(2): 67-69.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.008
Abstract( 130 )   HTML (   PDF(143KB)( 37 )

Objective:To investigate and analyse the clinical value of individual and comprehensive management (ICM) in treatment of acute pancreatitis (AP).Methods:From 1994 to 1997,68 patients with AP,including 30 cases of acute edematous pancreatitis (AEP) and 38 cases of acute necrotic pancreatitis (ANP) were treated by ICM.Results:The overall survival rates in AEP and ANP were 100% and 79%,respectively.For ANP,the survival rates in early operative group (in 2 weeks from onset) and ICM group were 75% and 84%,respectively.Conclusions:Introduction of individual and comprehensive management for treatment of acute pancreatitis and proper surgical schedule and modules may increase the survival rate of the disease.

The Value of Magnification Mammography with Spot Pressing in the Diagnosis of Breast Masses
Ge Lingyu, Ling Bingying, Xu Ping
Journal of ZheJiang University(Medical Science), 1999, 28(2): 70-72.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.009
Abstract( 50 )   HTML (   PDF(190KB)( 50 )

Objective:To evaluate the magnification mammography with spot-pressing technique in the diagnosis of breast masses.Method: We performed magnification mammography with spot-pressing technique on suspicious areas of routine mammographic examination in 72 cases, the results were compared with those of routine mammography.Results:magnification mammography with spot-pressing was significantly superior to routine mammography in the distinction of breast masses,especially in those lesions presented as mass-like consolidation or subtle calcifications or in dense mammary glands. A combination of the two techniques could lead to a higher diagnosis rate, especially early diagnosis rate.

The Clinical Application of Fetal Umbilical Arterial Blood Flow Waveform S/D Value measured by Doppler Sonography
Song Yili
Journal of ZheJiang University(Medical Science), 1999, 28(2): 73-74.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.010
Abstract( 56 )   HTML (   PDF(107KB)( 52 )

Objective:To monitor the fetal condition with measurement of resistance of placental vessels and placental function by Doppler Sonography.Methods:The systolic peak velocity (S) and end diastolic velocity (D) were measured by Doppler Sonography at three sites along the umbilical cord:near the fetal abdominal wall insertion (A),in the freefloating midcord (M) and near the placental site (P). The S/D ratio at each site was calculated.Results:In normal cases the mean values of S/D ratio measured at different sites were not the same, being highest at A, lowest at P. With the increase of gestational age, the S/D value steadily decreased. 32 cases with hypertension of pregnancy showed high S/D value at three sites. In 30 cases the S/D value at the site of umbilical cord around neck was similar to that of M.Conclusions:We therefore consider that the use of S/D ratio measurement by Doppler ultrasonography is a relatively effective method for the surveillance of high risk fetus.

The Application of Two dimensional Ultrasonography and Color Doppler in Diagnosis of the Testis Torsion
Zhao Junkang, Jiang Tianan, Zheng Zhilan
Journal of ZheJiang University(Medical Science), 1999, 28(2): 75-77.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.011
Abstract( 55 )   HTML (   PDF(144KB)( 34 )

Objective:To study the image characteristics of two-dimensional ultrasonography and color doppler of the testis torsion and to differentiate testis torsion from other scrotal emergency.Methods:The morphologic changes of testis and internal echo were displayed with two-dimentional ultrasound, and the blood perfusion in testis was observed with color doppler. The results were compared with those of operation and pathology or clinical follow up.Results:The characteristics of sonography of testis torsion were as follows:①The position changes of testis and epididymis in scrotum.②The clear reduction or disappearing of blood supply in testis.③The whole enlargements of testis and epididymis.Conclusion:The color doppler ultrasonography has high accuracy, sensitivity and specificity, and it is a non-traumatic method and can judge the degree of torsion and offer proof for judging efficacy of operational result. It should be regarded as a first means for diagnosing torsion of testis.

The Diagnosis and Differential Diagnosis of Acute Radiation Injury
Gao Shenyong, Sun Wenji, Ye Anfang
Journal of ZheJiang University(Medical Science), 1999, 28(2): 78-80.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.012
Abstract( 43 )   HTML (   PDF(144KB)( 24 )

Objective:To study the diagnosis of acute radiation injury with clinical qualitative and quantitative analysis.Methods:In 4 cases of acute radiation injury,radiodosages were estimated,clinical manifestation,laboratory examination results and chromosomal aberration rates were compared and analysed.Results:The contrasting analyses showed that in 3 cases of accidental radiation injury the whole/part exposure to radiodosage was 1.78 Gy/30 Gy, 0.12 Gy/5.42 Gy and 0.014 Gy/2.80 Gy and their chromosomal aberration rates were 7.33% ,2.5% and 1.5% respectively.In one case of non accident radiation,the radiodosage was <0.05 Gy and his chromosome aberration rate was zero.Conclusion:The chromosomal aberration rate can be used as clinical qualitative and quantitative mark of acute radiation injury.

The Clinical Characteristics and Treatment of Colonic Primary Malignant Lymphoma
Zhang Hongzhi
Journal of ZheJiang University(Medical Science), 1999, 28(2): 81-82.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.013
Abstract( 48 )   HTML (   PDF(143KB)( 24 )

Objective:To study the clinical and pathological characteristics of colonic primary malignant lymphoma.Methods:Pathologically based on the Chengdu NHL classification (1985) and the new WHO classif ication,a review analysis of the clinical data in 14 cases with colonic primary malignant lymphoma were made.Results:No postoperative complications were found in 14 cases.Six cases survived more than 5 years with a total survival rate 42.8%,including 3 cases with low degree B-cell malignant lymph oma,one with moderate degree B-cell malignant lymphoma,one with high degree T-cell malignant lymphoma,and one with undifferentiated lymphoma.Conclusion:The prognosis of low degree B-cell malignant lymphoma seems to be better than that of high degree T-cell malignant lymphoma.

The Causes of Early Dislocation after Total Hip Replacement
Yan Shigui, Wu Qionghua, Fang Xiangqian
Journal of ZheJiang University(Medical Science), 1999, 28(2): 83-85.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.014
Abstract( 39 )   HTML (   PDF(143KB)( 36 )

Objective:To discuss the causes of early dislocation after total hip replacement, for the purpose of its prevention and proper treatment. Method:An analysis of 101 cases (113 hips) who received total hip replacement was made. Among them,7 cases who suffered from early hip dislocation were followed up.Result:Inappropriate postoperative transportation and rehabilitation were the main causes of early hip dislocation;conservative treatment got satisfactory result.Conclusion:The intactness of the soft tissue surrounding the hip joint was the main factor in maintaining the stability of hip joint postoperatively.

Clinical Observation in Posterior Capsule Opacification Treatment by Nd:YAG Laser
Chen Peiqing, Yao Ke, Du Xinghua
Journal of ZheJiang University(Medical Science), 1999, 28(2): 86-87.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.015
Abstract( 78 )   HTML (   PDF(145KB)( 71 )

Objective:To observe the clinical manifestation in patients with posterior capsule opacification (PCO) after treatment of capsulectomy by Nd:YAG laser.Methods:One hundred and twenty six patients (130 eyes) who developed PCO after cataract extraction with intraocular lens implantation were treated with capsulectomy by Nd:YAG laser.Results:87.69% of eyes improved their visual acuity.Damage of intraocular lens was found in 25 eyes.One eye developed persistent high intraocular pressure and retinal detachment was also found in one eye.Conclusion:Laser capsulectomy improved the visual acuity in patients with PCO.

The Application of Mean Corpuscular Volume and Morphology of urinary RBC in Differential Diagnosis of Pure Hematuria
Xu Kaishen, Shen Hanchao, Jiang Minqin
Journal of ZheJiang University(Medical Science), 1999, 28(2): 88-90.   https://doi.org/10.3785/j.issn.1008-9292.1999.02.016
Abstract( 47 )   HTML (   PDF(147KB)( 34 )

Objective:To develop a new diagnostic technology to differentiate glomerulur from non-glomerulur hematuria.Methods:The mean corpuscular volume (MCV) of urinary RBC was measured with blood cell auto-counting machine.At the same time,the morphology of urinary RBC was observed carefully with microscope.Results:MCV of urinary RBC derived from glomerulus was 62.2±5.2 fl;the ratio of abnormal morphology RBC was 46.7±12.0%. On the contrary, those derived from non-glomerulus were 89.4±5.8 fl and 10.0±5.0% respectively.There were statistically significant differences between them (P<0.01).The correspondence rates of diagnosis for hematuria derived from both glomerulus and non glomerulus were 95% (38/40) and 98% (48/49) respectively.Conclusion:The technology was useful for clinical diagnosis of types of hematuria.

16 articles