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, Volume 40 Issue 3 Previous Issue    Next Issue
Screening strategies for colorectal cancer
Journal of ZheJiang University(Medical Science), 2011, 40(3): 233-236.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.001
Abstract( 329 )   HTML (   PDF(1336KB)( 261 )
Screening of Tag SNPs and prediction of their potential function in genetic studies of complex diseases
Journal of ZheJiang University(Medical Science), 2011, 40(3): 237-244.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.002
Abstract( 430 )   HTML (   PDF(1926KB)( 620 )
Expression of caspase apoptosis pathway genes mRNA in colorectal cancer and polypus
Journal of ZheJiang University(Medical Science), 2011, 40(3): 245-251.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.003
Abstract( 266 )   HTML (   PDF(1025KB)( 195 )
Objective: To investigate mRNA expression of caspase apoptosis pathway genes in colorectal cancer,polypus and normal mucosa.
Methods: Nineteen patients with colorectal cancer,86 patients with polypus and 10 normal controls were enrolled from 2008 to 2010.Fluorescence quantitative RT-PCR was performed to detect the mRNA expression of caspase apoptosis pathway genes (caspase-2,-3,-6,-7,-8,-9 and -10) in colorectal cancer,polypus and normal mucosa.
Results: There were no statistically significant differences of demographic characteristics between patients with colorectal cancer,patients with polypus and normal controls.Compared with normal control group,the mRNA expression of all selected genes except for caspase-3 were lower; however,the P values did not reach statistic significance.Highly positive correlations were observed between mRNA expression of all selected genes except caspase-9.
Conclusions: There are no significant changes in mRNA expression levels of caspase apoptosis pathway genes from normal mucosa to polypus to cancer.The mRNA expressions of most caspase pathway genes are highly correlated with each other.
Association of XPC gene polymorphisms with breast cancer risk
Journal of ZheJiang University(Medical Science), 2011, 40(3): 252-258.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.004
Abstract( 345 )   HTML (   PDF(1132KB)( 221 )
Objective: To access the association of xeroderma pigmentosum group C (XPC) Lys939Gln (A/C) and Ala499Val (C/T) polymorphisms with breast cancer risk in a Chinese Han population.
Methods: 173 patients with breast cancer and 171 matched controls in terms of habitation and age (±5 years) were included in this population-based case-control study.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was employed to genotyping the Lys939Gln and Ala499Val polymorphisms.Associations of genotypes of Lys939Gln and Ala499Val with breast cancer risk were evaluated using unconditional logistic regression model.Associations between XPC haplotypes and breast cancer risk were estimated by Haplo.Stats package.
Results: No significant associations were observed both in individual SNPs and haplotype analyses.However,there was a significant interaction between XPC Lys939Gln polymorphism and menopausal status (P=0.032).
Conclusion: The XPC Lys939Gln polymorphism may modulate breast cancer susceptibility jointly with the menopausal status.
Association of CASP3 polymorphisms and its haplotypes with susceptibility of breast cancer
Journal of ZheJiang University(Medical Science), 2011, 40(3): 259-264.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.005
Abstract( 445 )   HTML (   PDF(1128KB)( 228 )
Objective: To investigate the association of Caspase3 (CASP3) polymorphisms with susceptibility of breast cancer in Chinese Han population.
Methods: In this population-based case-control study, 251 cases with breast cancers and 251 matched controls in terms of habitation and age (±5 years) were recruited.Rs4647693,rs2696056,rs4647610 were selected as TagSNPs in CASP3 gene and genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.The haplotype distribution was estimated and compared by PHASE software.
Results: There was significant association between menarche age and breast cancer (P=0.007),as well as the early pregnancy age and breast cancer (P=0.002).No significant differences were detected in the distribution of CASP3 genotype and haplotype frequencies between breast cancer patients and controls.The GGA was the most common haplotype both in cases and controls.
Conclusion: CASP3 polymorphisms and its haplotypes were not related to the susceptibility of breast cancer.
Association of miR-605 and miR-149 genetic polymorphisms with related risk factors of lung cancer susceptibility
Journal of ZheJiang University(Medical Science), 2011, 40(3): 265-271.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.006
Abstract( 382 )   HTML (   PDF(1030KB)( 294 )
Objective: To explore association of miR-149 and miR-605 polymorphisms with other risk factors of lung cancer susceptibility among Chinese population.
Methods: Two hundred and forty-four patients with lung cancer and 243 cancer-free controls matched by age and sex were enrolled from 2002 to 2008.Peripheral venous blood samples were collected from all subjects.Single nucleotide polymorphisms (SNPs) of miR-149 and miR-605 were genotyped by PCR-RFLP.Multiple-variable logistic regression model was used to assess the association of SNPs and cancer related risk factors for lung cancer.
Results: There was not significant association of SNPs of miR-149 and miR-605 with lung cancer.A marginal significance was observed while the males with at least one G allele of miR-605 had higher risk of lung cancer (OR=1.5,95%CI: 1.0-2.3) than those with AA genotype.Increased frequency of smoking was associated with lung cancer risk.Compared with no-smoker,the subjects with <20 and ≥20 cigarettes/day had higher risk of lung cancer: OR (95%CI)=1.7(1.0-3.0) for <20 cigarettes,OR (95%CI)=4.2(2.3-7.6) for ≥20 cigarettes.There was no interaction between two genes and smoking on lung cancer.
Conclusions: miR-149 polymorphisms may not affect lung cancer susceptibility.miR-605 gene mutant might be increase the risk of lung cancer among males.Cigarette smoking increased a risk of lung cancer,but there were not interactive effects between two gene and smoking on lung cancer.
Optimization of sequential screening scheme in prevention of colorectal neoplasm
Journal of ZheJiang University(Medical Science), 2011, 40(3): 272-275.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.007
Abstract( 461 )   HTML (   PDF(1001KB)( 393 )
Objective: To improve early diagnosis rate and reduce the incidence rate of colorectal cancer,through the application of optimized sequential screening scheme for colorectal neoplasm in general population.
Methods: Quantitative risk assessment by questionnaires survey and fecal occult blood test (FOBT) were used to proceed preliminary screening among people aged 40 to 74.Electronic colonoscopy was applied to examine the whole colon and rectum among identified high-risk subjects.The detected cases received treatment for colorectal cancer,adenomatous polyps or non-adenomatous polyps.The early diagnosis rate and incidence rate of colorectal cancer were evaluated and compared with those before screening.
Results: With application of optimized sequential screening schemes,various types of colorectal lesions were detected in 1 117 subjects,including 69 cases of colorectal cancer,701 cases of colorectal adenoma and 211 cases of advanced adenoma.The early diagnosis rate of colorectal cancer was increased by 58.19%,and its incidence rate also decreased significantly.
Conclusion: The optimized sequential screening scheme is simple,economical,efficient in colorectal cancer screening of general population.
Comparison of mail and meeting forms in evaluation of Delphi study
Journal of ZheJiang University(Medical Science), 2011, 40(3): 276-280.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.008
Abstract( 371 )   HTML (   PDF(1066KB)( 576 )
Objective: To evaluate and compare mail and meeting forms in evaluation of Delphi study.
Methods: Delphi study by mail and meeting approaches was used to determine the health information dataset.Experts were required to grade the listed items through three indexes: importance,necessity and availability.Study duration,coefficient of variation of items,authority coefficient and coordination coefficient of the experts′ opinion of two forms of study were calculated and compared.
Results: The study duration was four months through mail form and 2 days through meeting.Compared with the first round,the coefficient of variation decreased (P<0.001,all of the three indexes by two forms),and the cooperation index increased (P<0.005) in the second round.The experts′ opinions were easier to be consistent through meeting than through mail(P<0.033).And the authority coefficient by meeting consultation(0.83±0.05) was higher than that by mail (0.77±0.03) (P=0.001).
Conclusion: Both mail and meeting forms of Delphi study can determine the health information dataset,but meeting consultation is better and requires shorter study duration.
Acceptability of pre-exposure prophylaxis among female sex workers in Xinjiang
Journal of ZheJiang University(Medical Science), 2011, 40(3): 281-285.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.009
Abstract( 581 )   HTML (   PDF(1013KB)( 262 )
Objective: To assess the acceptability of pre-exposure prophylaxis (Pr-EP) among the female sex workers in Xinjiang.
Methods: A volunteer-based,anonymous and one-to-one questionnaire survey was conducted in 762 female sex workers (FSW) in Urumqi and Kelamayi of Xinjiang Uyghur Autonomous Region.
Results: Among 762 FSW surveyed,673 (88.32%) was not aware of pre-exposure prophylaxis with an awareness rate of 11.55%.The awareness rate of FSWs working in high-end entertainment venues was higher than that of FSWs working in medium-low end entertainment venues(P<0.001).Five hundred and twenty eight FSWs (69.29%) were willing to take Pr-EP,145 (19.03%) were unwilling to take the medicine and 89 (11.68%) were possible to use the Pr-EP.There was no significant difference in willingness of using Pr-EP among FSWs working in high and medium-low end entertainment venuew(P=0.285).The subjects who were willing to take Pr-EP mainly concerned of the drug security,effectiveness and cost.The main reasons for not willing to take Pr-EP were: not having risk of infecting HIV,suspecting effectiveness of Pr-EP and worrying about side effects.
Conclusions: The acceptability to use Pr-EP in female sex workers of Xinjiang is relatively high and the drug security,effectiveness and cost will influence the promotion and application of Pr-EP in the future.
Repetitive transcranial magnetic stimulation combined with antidepressant medication in treatment of first-episode patients with major depression
Journal of ZheJiang University(Medical Science), 2011, 40(3): 286-290.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.010
Abstract( 394 )   HTML (   PDF(1016KB)( 498 )
Objective: To assess the early therapeutic and cognitive effect of repetitive transcranial magnetic stimulation (rTMS) combined with antidepressant medication in treatment of first-episode patients with major depression.
Methods: Sixty first-episode depressed inpatients aged 18-45 y,who met the DSM-IV clinical criteria for major depressive episode were randomly assigned to citalopram treatment (20 mg/d) in combination with a two-week period of either rTMS (study group)or sham procedure (control group) on left dorsal-lateral prefrontal cortex (10 Hz,90% motor threshold).The Hamilton Depression Rating Scale (HAMD) was used to assess the severity of depression.The Wisconsin Card Sorting Test (WCST) and Continuous Performance Test (CPT) were used to assess cognitive function of depression.
Results: The response rate was significantly greater in the study group compared to the control group after treatment (57% vs 29%,P<0.05).The HAMD scores significantly declined after treatment in two groups,and the study group showed lower scores compared to the control group after 2 weeks (P<0.01).Neuropsychological assessments showed that there was no significant difference between the two groups except for the significant improvement in the categories on WCST in study group compared to the baseline(P<0.05)and the control group(P<0.05)after 2 weeks treatment.No serious event occured in the patients during the rTMS study.
Conclusion: 10 Hz rTMS enhances early effects of citalopram and improves cognitive function in first-episode major depressive patients.
Construction and expression of recombinant adenovirus containing human catalase gene in vitro
Journal of ZheJiang University(Medical Science), 2011, 40(3): 291-296.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.011
Abstract( 273 )   HTML (   PDF(1330KB)( 199 )
Objective: To construct the adenovirus vector containing recombinant human catalase (CAT) and to express the recombinant gene in vitro.
Methods: Total RNA was extracted from human leukocytes and full-length human CAT cDNA was obtained with RT-PCR method.The CAT gene was cloned into pcDNA3.1(+) vector and pcDNA3.1(+)CAT was constructed.The positive clones were confirmed by the restriction enzyme digestion and gene sequencing.The CAT gene was cloned into the entry vector pENTR1A,and pENTR1A-CAT vector was constructed.By LR reaction pENTR1A-CAT and pAd/CMV/V5-DEST was recombined in vitro,and the recombinant adenovirus pAd/CMV/V5-DEST-CAT was obtained.The positive pAd/CMV/V5-DEST-CAT was confirmed by sequencing and transfected into 293A cells with Pac I linearization and Lipofectamine 2 000,and the recombinant virus particles were packaged and amplified in the cells.The expression of CAT protein and CAT enzyme activities of the recombinant virus were determined by Western blot and 240 nm UV absorption methods.
Results: High expression of recombinant adenovirus was obtained and the expressed human catalase had high enzyme activity.
Conclusions: Ad/CMV/V5-DEST-CAT vector containing human catalase gene has been constructed successfully; and the expressed enzyme in 293A cells has high activity.
Codon optimization of recombinant staphylococcal enterotoxin O enhances the expression level in Escherichia coli
Journal of ZheJiang University(Medical Science), 2011, 40(3): 297-303.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.012
Abstract( 271 )   HTML (   PDF(1723KB)( 203 )
Objective: To enhance the expression level of staphylococcal enterotoxin O(SEO) by optimization of rare codons.
Methods: The gene of mature SEO (His-tag included) was cloned to pET28a,and 15 rare codons on the gene were optimized by PCR technology.These recombinant plasmids then were transformed into E.coli BL21(DE3),respectively.After IPTG induced,the expression levels of those mutants were analyzed by SDS-PAGE.The proteins were purified and their bioactivities were determined.
Results: After the optimization of rare codons,the expression levels were increased from 7.49% to 19.8% in total cell proteins.The optimized SEO had bioactivity to stimulate the proliferation of murine lymphocytes,which was equivalent to that of non-optimized SEO in vitro.
Conclusion: Optimization of rare codons can enhance the expression of SEO effectually.
Role of mitochondrial calcium uniporter in cardioprotection induced by ischemic postconditioning in isolated rat heart
Journal of ZheJiang University(Medical Science), 2011, 40(3): 304-308.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.013
Abstract( 306 )   HTML (   PDF(1380KB)( 282 )
Objective: To investigate the role of mitochondrial calcium uniporter in cardioprotection elicited by ischemic postconditioning (Postcond).
Methods: Male Sprague-Dawley rats were used for Langendorff isolated heart perfusion.The hearts subjected to global ischemia for 30 min followed by 120 min of reperfusion.Left ventricular developed pressure (LVDP),maximal rise/fall rate of left ventricular pressure (±dP/dtmax) were measured.The level of lactate dehydrogenase (LDH) in the coronary effluent was measured spectrophotometrically,the content of formazan of myocardium was also measured at the end of reperfusion.
Results: Compared to I/R group,Postcond had an significant increase in the mechanical function of the left ventricle,with LDH release reduced and the content of formazan increased.Spermine,the opener of mitochondrial calcium uniporter,deteriorated the mechanical function of left ventricle and decreased the formazan content,and increased LDH release.Ruthenium red,the inhibitor of mitochondrial calcium uniporter,increased the mechanical function of the left ventricle,decreased the LDH release,but the content of formazan was not increased.
Conclusion: The inhibition of mitochondrial calcium uniporter is involved in the mechanisms of ischemic postconditioning.
DNA methylation of ZIC1 and KLOTHO gene promoters in colorectal carcinomas and its clinicopathological significance
Journal of ZheJiang University(Medical Science), 2011, 40(3): 309-314.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.014
Abstract( 422 )   HTML (   PDF(1105KB)( 241 )
Objective: To determine DNA methylation status of ZIC1 and KLOTHO gene in colorectal carcinomas and its relationship with clinicopathological features of patients.
Methods: The mRNA expression of ZIC1 and KLOTHO genes in colorectal carcinomas was detected by real-time quantitative RT-PCR,and the promoter methylation status was detected by methylation specific PCR (MSP).The relationship of ZIC1 and KLOTHO methylation status with clinicopathological features of colorectal carcinoma was analyzed.
Results: The mRNA expression levels of ZIC1 and KLOTHO genes were significantly down-regulated in tumor tissues when compared to adjacent nontumor tissues (P<0.001). ZIC1 and KLOTHO methylation was detected in 80.0% (20/25) and 76.0% (19/25) of colorectal tumor tissues, respectively, and the both positive rate was 64.0% (16/25).
Conclusions: The down-regulated expression of ZIC1 and KLOTHO in colorectal carcinoma may relate to promoter methylation.The detection of methylation of ZIC1 and KLOTHO gene potentially provides biomarkers for diagnosis of colorectal carcinoma.
Effects of mice body temperature on pressure inside plethysmograph
Journal of ZheJiang University(Medical Science), 2011, 40(3): 315-320.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.015
Abstract( 337 )   HTML (   PDF(1470KB)( 283 )
Objective: To observe temperature and pressure changes inside plethysmograph produced by body temperature of anesthetized mice.
Methods: The temperature and pressure changes inside whole body plethysmograph generated from anesthetized mice were compared with those from dead mice.The temperature and pressure changes inside body chamber and head chamber of double-chamber with anesthetized mice in body chamber were synchronously measured.The respiratory frequencies and amplitudes of mice inside two kinds of head-out plethysmographes were synchronously measured.One of these two plethysmograhes kept sealed all the time and the other was opened to the atmosphere for 1 min every 2 min.
Results: Temperature and pressure of air in the anesthetized mice chamber increased 1.18℃ and 2.710 mmHg within 6 min,and data from dead mice were 1.17℃ and 2.671 mmHg.There were no significant differences between these two groups.The temperature inside body chamber increased 1.92℃ in 20 min and the pressure was 5.554 mmHg,which were significantly higher than those of head chamber (0.09℃ and 0.627 mmHg).The respiratory frequencies of mice in the sealed head-out plethysmograph increased from 125.04 per min to 168.45 per min,and amplitudes of pressure changes generated from mice breath decreased from 1.090 mmHg to 0.883 mmHg.Significant differences occurred between different observation time points.Meanwhile respiratory frequencies in the open head-out plethysmograph were around 120 per min and amplitude of pressure changes kept about 1 mmHg.There were no significant differences between different time points.
Conclusion: Increase of temperature and pressure inside pressure whole-body plethysmograph are mainly from body temperature of mice,and the increased pressure significantly influences respiration of mice.
Clinical characteristics and in vivo confocal microscopic imaging of Fleck corneal dystrophy
Journal of ZheJiang University(Medical Science), 2011, 40(3): 321-326.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.016
Abstract( 244 )   HTML (   PDF(1596KB)( 206 )
Objective: To investigate the clinical characteristics and the corneal morphological abnormalities in Fleck corneal dystrophy.
Methods: Eighteen eyes with Fleck corneal dystrophy of 9 patients from two unrelated families were examined by slit-lamp biomicroscopy,Cochet-Bonnet esthesiometer and in vivo confocal microscopy.The corneal cells,nerves and stromal deposits were analyzed quantitatively with NAVIS software.
Results: Slit-lamp biomicroscopy of the Fleck corneal dystrophy revealed that bilateral small grey-white fleck-like or wreath-like opacities were scattered in all layer of the corneal stroma from the center to the periphery,the intervening stroma between the lesions was clear.In vivo confocal microscopy identified that the opacities appeared as doughnut-like or nephroid-like deposits approximately 70.6 μm×110.3 μm in size,(1.6± 0.4)/frame in density and involved about (438.4±22.0) μm of the corneal stroma,with hyperreflective dot-like intracellular particles,measuring 2 to 18 μm in diameter.In two eyes of 1 patient associated with decreased corneal sensation,confocal images showed that abnormal hyperreflective deposits involved the Bowman′s layer and the branch and density of the subbasal nerve was reduced.The other eyes with normal corneal sensation appeared normal in the morphology and the density of the corneal nerves.
Conclusions: Fleck corneal dystrophy has typical clinical characteristics.In vivo confocal microscopy allows to study the morphological changes of the cornea at cellular level,which is valuable for the clinical diagnosis and evaluation of the corneal dystrophy.Decreased corneal sensitivity in Fleck corneal dystrophy is probably caused by corneal stromal deposits involved in the Bowman′s layer.
Efficacy of bismuth-based quadruple therapy as first-line treatment for helicobacter pylori infection
Journal of ZheJiang University(Medical Science), 2011, 40(3): 327-331.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.017
Abstract( 633 )   HTML (   PDF(1016KB)( 763 )
Objective: To evaluate the efficacy of bismuth-based quadruple therapy as the first-line treatment for H.pylori infection.
Methods: A total of 136 patients with H.pylori related peptic ulcer or chronic gastritis were randomized into two groups: 67 patients in bismuth-based quadruple group received esomeprazole 20 mg,clarithromycin 0.5 g,amoxicillin 1.0 g,and bismuth potassium citrate 220 mg for 7 d; 69 patients in standard triple group received esomeprazole 20 mg,clarithromycin 0.5 g and amoxicillin 1.0 g for 7 d. Outcome of eradication therapy was assessed by 14C-UBT.On ITT and PP analysis,calculating the cost-effectiveness ratio (C/E) and the incremental cost-effectiveness ratio(△C/△E).
Results: On ITT and PP analysis,the eradication rates of the quadruple therapy group were 82.09% and 88.71%,and those of the triple therapy group were 66.67% and 73.02% (P<0.05).The cost-effectiveness ratio of two groups was 4.15 and 4.82; The incremental cost-effectiveness ratio of quadruple therapy group was 1.02 as against triple therapy group.
Conclusion: Compared to the standard triple therapy regimen,the bismuth-containing quadruple therapy regimen has higher eradication rate and cost-effectiveness,which can be recommended as the fist-line treatment for H.pylori infection.
Research progress on barrel cortex and its plasticity
Journal of ZheJiang University(Medical Science), 2011, 40(3): 332-337.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.018
Abstract( 686 )   HTML (   PDF(1598KB)( 612 )
Synaptic plasticity of barrel cortex is one of the most widely studied topics in neuroscience in recent years.The primary somatosensory cortex of the rodent has a good topology character,which provides an ideal experimental model for plasticity study.This system displays very strong experience-dependent plasticity both during development and in adulthood.The changes of sensory cortex′s neural circuit can induce experience-dependent plasticity.In the synaptic level,thalamocortical synapse is considered to be the main location of plasticity.In the circuit level,both synapses from layer 4 to layer 2/3 and those within layer 2/3 are also the necessary parts of achieving synaptic plasticity in primary somatosensory cortex.The GABAergic inhibitory circuit may be involved in this plasticity of S1,but the exact mechanism remains unknown.
Research progress on living quality of children conceived by assisted reproductive technology
Journal of ZheJiang University(Medical Science), 2011, 40(3): 338-343.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.019
Abstract( 366 )   HTML (   PDF(1080KB)( 383 )
With the development of assisted reproductive technique (ART), children born following ART now form a sizeable subgroup of the population, so assessment for the safety of ART becomes more urgent than ever before. The health problems about living quality of children conceived by ART mainly focus on perinatal outcome and short-term follow-up study, including multiple pregnancies, low birth weight and prematurity in singleton births, congenital malformations, imprinting disorders, neurodevelopmental outcome, neonatal outcomes, physical and mental health, as well as cancer risks. In this review, we will discuss the health problems of these children, to provide data for further study.
Early lung cancer complicated with lungs embolism :A case report
Journal of ZheJiang University(Medical Science), 2011, 40(3): 344-346.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.020
Abstract( 384 )   HTML (   PDF(1975KB)( 199 )
Treatment of epithelial implantation cyst of iris by penetrating keratoplasty:Report of one case
Journal of ZheJiang University(Medical Science), 2011, 40(3): 347-348.   https://doi.org/10.3785/j.issn.1008-9292.2011.03.021
Abstract( 254 )   HTML (   PDF(1162KB)( 194 )
21 articles