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Prospects for research on small molecular targeted drugs in treatment of acute myeloid leukemia
Journal of ZheJiang University(Medical Science), 2012, 41(5): 469-472.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.001
Abstract( 1078 )   HTML (   PDF(528KB)( 444 )
Synergistic effect of histone deacetylase inhibitor suberoylanilide hydroxamic acid with imatinib on K562 cells
Journal of ZheJiang University(Medical Science), 2012, 41(5): 473-478.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.002
Abstract( 581 )   HTML (   PDF(1275KB)( 295 )
Objective: To investigate synergistically killing effect of histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) combined with imatinib on human chronic myeloid leukemia (CML) cell line.
Methods: K562 cells were co-treated with SAHA and imatinib.Cell growth was measured by MTT assay.Apoptosis was determined using Hoechst staining apoptosis detection kit and flow cytometric analysis.Activation of Caspase pathway,expression of Bcr-Abl and its downstream target genes,and expression of anti-apoptotic proteins were detected by Western blot.
Results: SAHA synergized the cytotoxicity of imatinib against leukemia K562 cells,concomitantly with increased apoptosis and enhanced activation of Caspase-3,-8 and PRAP.The combination therapy resulted in significantly lower levels of Bcr-Abl,phosphorylated Bcr-Abl compared to treatment with either SAHA or imatinib alone.Furthermore,the co-treatment resulted in down-regulation of anti-apoptotic protein Mcl-1 expression.Also,marked down-regulated expression of JAK2,STAT5,and phosphorylated STAT5 was detected in the combination therapy.
Conclusions: Combining HDAC inhibitor SAHA with imatinib can kill CML cells synergistically by inhibiting cell growth and inducing apoptosis, which is associated with activation of Caspase pathway and regulation of anti-apoptotic proteins.
Killing effect of aurora kinase inhibitor ENMD-2076 on acute myelogenous leukemia cells
Journal of ZheJiang University(Medical Science), 2012, 41(5): 479-484.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.003
Abstract( 736 )   HTML (   PDF(1663KB)( 303 )
Objective: To investigate the effect of aurora kinase inhibitor ENMD-2076 on human acute myelogenous leukemia (AML) cell lines.
Methods: AML THP-1 and Kasumi-1 cells were treated with ENMD-2076 for 24 h and 48 h,respectively.Cell growth was measured by MTT assay.Apoptosis was determined using Hoechst staining apoptosis detection kit.Activation of Caspase pathway and expression of apoptosis regulator proteins were detected by Western blot.
Results: ENMD-2076 significantly induced growth arrest and apoptosis in THP-1 and Kasumi-1 cells.Enhanced apoptosis was observed in ENMD-2076 group evidenced by strong activation of Caspase-9,Caspase-3 and PARP.Furthermore,the ENMD-2076 treatment resulted in down-regulation of anti-apoptotic protein Mcl-1 expression.Also,up-regulated expression of pro-apoptotic protein Bak,Bad and Bax was detected after ENMD-2076 treatment.
Conclusion: ENMD-2076 can kill effectively AML cells by inhibiting cell growth and inducing apoptosis,which is associated with activation of Caspase pathway and regulation of pro-apoptotic and anti-apoptotic proteins.
Cytotoxicity of homoharringtonine on leukemic stem-like cells in AML cell line KG-1
Journal of ZheJiang University(Medical Science), 2012, 41(5): 485-490.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.004
Abstract( 781 )   HTML (   PDF(2387KB)( 333 )
Objective: To investigate the effect of homoharringtonine (HHT) on leukemic stem-like cells (LSC) in human acute myeloid leukemia (AML) cell lines.
Methods: The phenotypes of AML cell lines U937,Kasumi-1,and KG-1 cells were analyzed by flow cytometry (FACS).The effect of HHT on leukemia stem-like cells with immunophenotype of CD34+CD38-CD96+ was detected with FACS.Cell growth was measured by MTT assay.Activation of Caspase pathway and expression of apoptotsis-related regulator proteins were examined by Western blotting.
Results: FACS demonstrated that the 69% of KG-1 cells expressed LSC phenotype CD34+CD38-CD96+,while 26.7% on Kasumi-1 cells expressed this marker.In contrast,U937 cells showed CD96 negative.HHT significantly inhibited cell growth of KG-1 cells with an IC50 of 16.9 ng/ml at 48 h.The ratio of CD34+CD38-CD96+ cells decreased from 63.6% to 17.1% after HHT treatment.Enhanced apoptosis was demonstrated in HHT group evidenced by strong activation of Caspase-9,Caspase-3 and PARP.HHT treatment resulted in down-regulation of expression of anti-apoptotic protein BCL-2 and phosphated-Akt.
Conclusion: HHT can effectively kill the leukemic stem-like cells in human AML cell line KG1 by inhibiting cell growth and inducing apoptosis which is associated with activation of Caspase pathway and down-regulation of anti-apoptotic proteins and phosphated-Akt.
Antitumor activity of histone deacetylase inhibitor suberic bishydroxamate on acute myeloid leukemia cell lines
Journal of ZheJiang University(Medical Science), 2012, 41(5): 491-497.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.005
Abstract( 605 )   HTML (   PDF(1881KB)( 338 )
Objective: To investigate the effect of histone deacetylase inhibitor suberic bishydroxamate (SBHA) on human acute myeloid leukemia (AML) cell lines.
Methods: AML U937,KG-1 and Kasumi-1 cells were treated with SBHA.Cell growth was measured by MTT assay.Apoptosis was determined using flow cytometry.Activation of Caspase pathway and expression of apoptosis regulator proteins were detected by Western blot.
Results: SBHA significantly induced growth arrest and apoptosis in U937,KG-1 and Kasumi-1 cells.Enhanced apoptosis was observed in SHBA group evidenced by strong activation of Caspase-9,Caspase-8 and Caspase-3.SHBA treatment resulted in down-regulation of anti-apoptotic protein Bcl-2 and Bcl-xl expression; down-regulated expression of antiapoptotic proteins survivin,XIAP and cIAP was also detected after SBHA treatment.
Conclusion: SBHA can effectively kill AML cells by inhibiting cell growth and inducing apoptosis,which is associated with the activation of Caspase pathway and regulation of apoptotic related proteins.
Effect of Evn-50 on cell growth and apoptosis in tamoxifen-resistance human breast cancer cell line MCF-7/TAM-R
Journal of ZheJiang University(Medical Science), 2012, 41(5): 498-505.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.006
Abstract( 641 )   HTML (   PDF(1942KB)( 365 )
Objective: To investigate the effect of Evn-50 extracted from Vitex negundo on human breast cancer cell line MCF-7 and MCF-7/TAM-R cells in vitro.
Methods: MCF-7 and tamoxifen-resistant MCF-7/TAM-R cells were treated with Evn-50,tamoxifen or combination of Evn-50 and tamoxifen.Cell proliferation inhibition rates were determined by MTT assay.The apoptosis rate and the change of cell cycle were detected by PI staining flow cytometry.Protein expression of phospho-MAPK 44/42 (Thr202/Tyr204),MAPK P44/42,phospho-AKT (Ser473) and AKT were detected with Western blotting.
Results: The viability of MCF-7 cells was decreased in combination group [(28.65±11.43)%] and Evn-50 group [(53.02±15.14)%] compared with TAM group (P<0.01). The cell viability of MCF-7/TAM-R in combination group [(42.11±14.30)%] was significantly lower than that in TAM group [(92.18±13.16)%] (P<0.01).The cell apoptosis rate was dependent on the time of treatment in all groups,the effects on apoptosis and G2/M phase cells were most prominent at 72 h (P<0.01) .Western blotting revealed that protein levels of phosphorylated AKT and p-MAPK44/42 decreased,while the expression of total AKT and MAPK44/42 was stable.In MCF-7/TAM-R cells,the expression of phosphorylation of AKT and MAPK44/42 protein was not changed in Evn-50 or TAM alone group,but significantly inhibited in the combination group at 72 h. Conclusions: Evn-50 can inhibit cell growth and induce apoptosis in MCF-7 and MCF-7/TAM-R cells,it can reverse tamoxifen-resistance of MCF-7/TAM-R cells.The mechanisms may be related to the down-regulation of phosphorylated ERK1/2 in MAPK signal pathway and phosphorylated AKT in AKT signal pathway.
Establishment of a transgenic cell line with stable expression of human CD14
Journal of ZheJiang University(Medical Science), 2012, 41(5): 506-511.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.007
Abstract( 644 )   HTML (   PDF(1401KB)( 369 )
Objective: To establish a transgenic cell line with stable expression of CD14.
Methods: Total RNA extracted from peripheral blood mononuclear cells was treated with RNAase-free DNAase,the human CD14 gene was cloned and sequenced through the RT-PCR,T-A clone techniques and ABI PRISM377 machine.Eukaryotic expressional vector pcDNA3.1(+)/CD14 was constructed by cleaving with double restriction endonuleases EcoRⅠ/XbaⅠ and ligating with T4 ligase.The human cervical cancer cell line Hela was transfected with the positive recombinant plasmid pcDNA3.1(+)/CD14 using superfect transfection reagent.Positive clones were selected by G418 at a concentration of 0.5 μg/μl and the expression of human CD14 on the transfected Hela cells was confirmed by quantitative PCR and immunofluorescent assay.
Results: There was significantly difference om expression of CD14 mRNA between the blank pcDNA3.1(+) transfected cells and pcDNA3.1(+)/CD14 transfected cells (P<0.01).The fluorescence was significantly stronger on the stable cell line Hela-CD14 than that on the transiently transfected Hela cells,and no visible fluorescence was observed in blank vector transfected cells.
Conclusions: The transfectant cell line Hela-CD14 with stable expression of human CD14 has been successfully established, which can be used to study human CD14 molecular and CD14-associated monocyte/macrophage cell diseases.
A case-control study on risk factors of female breast cancer in Zhejiang province
Journal of ZheJiang University(Medical Science), 2012, 41(5): 512-518.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.008
Abstract( 680 )   HTML (   PDF(742KB)( 317 )
Objective: To investigate the risk factors on female breast cancer in Zhejiang province.
Methods: A case-control study was conducted in 200 cases of female breast cancer with histopathological diagnosis and 200 matched controls from Zhejiang province.
Results: Univariate conditional logistic regression showed that family history of malignant tumor and breast cancer,housing decoration in last 10 years,mammary hyperplasia,adverse life events,bra with steel rings,sleeping with bra,high fat and pickle intake,poor sleep were positively related to breast cancer; whereas environmental friendly decoration materials,long decoration time interval,workplace condition,more lactation and parity,high fruits intake,sufficient sleep were negatively related to breast cancer.Multivariate conditional logistic regression analysis showed that the risk factors included family history of other tumors [odds ratio (OR)= 1.571,95% confidence interval(CI):1.029-2.396],mammary hyperplasia (OR=3.066,95%CI:1.834-5.126), job-related life events (OR=4.575,95%CI:1.690-12.390),the death of a loved one (OR=2.555,95%CI:1.475-4.424),wearing bra at night (OR=1.902,95%CI:1.177-3.072),high fat intake (OR=2.709,95%CI:1.546-4.749) and salted food (OR=2.460,95%CI:1.300-4.653).Factors as environmental friendly decoration materials (OR=0.517,95%CI:0.339-0.789),workplaces condition (OR=0.430,95%CI:0.243-0.762),more lactation (OR=0.109,95%CI:0.013-0.896),enough sleep (OR=0.424,95%CI:0.205-0.880) were protective factors.
Conclusion: Hereditary,psychological factors,lifestyle,environment and diet related factors are significantly associated with risk of breast cancer.
Construction of pBIFC-VN173-CXCR4 and pBIFC-VC155-NT21MP eukaryotic expression plasmids and their interaction in living cells
Journal of ZheJiang University(Medical Science), 2012, 41(5): 519-526.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.009
Abstract( 891 )   HTML (   PDF(2923KB)( 467 )
Objective: To construct pBIFC-VN173-CXCR4 and pBIFC-VC155-NT21MP eukaryotic expression plasmids and to investigate the interaction of chemokine receptor 4 (CXCR4) and viral macrophage inflammatory protein-Ⅱ(vMIP-Ⅱ) N terminal 21 peptides (NT21MP) in living cells.
Methods: DNA fragment encoding NT21MP was chemically synthesized and inserted into BiFC eukaryotic expression vector pBIFC-VC155.The full length of CXCR4 DNA fragment was amplified by RT-PCR from SKBR3 cells and inserted into BiFC eukaryotic expression plasmid pBIFC-VN173.Two recombinant vectors were identified by restriction enzyme digestion and DNA sequencing.The recombinant vectors were cotransfected into Africa green monkey kidney fibroblast COS-7 cells by using Lipofectamine 2000.The interaction of NT21MP and CXCR4 was detected by bimolecular fluorescence complementation (BiFC) assay.
Results: The restriction enzyme digestion and DNA sequences and open read frames of two vectors were consistent with experiment design.The BiFC plasmids were successfully cotransfected into the target cells and expressed.The strong BiFC signals were detected in pBIFC-VN173-CXCR4 and pBIFC-VC155-NT21MP cotransfected cells and the fluorescence signal was located in the cytoplasm.
Conclusions: The eukaryotic expression plasmids for BiFC assay are successfully constructed.The interaction of NT21MP and CXCR4 in living cells can be detected by using this technology.
Effects of snakegourd root polysaccharide on apoptosis of MCF-7 cells
Journal of ZheJiang University(Medical Science), 2012, 41(5): 527-534.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.010
Abstract( 720 )   HTML (   PDF(2507KB)( 267 )
Objective: To investigate the effects of snakegourd root polysaccharide on apoptosis of human breast cancer cells (MCF-7 cells).
Methods: Colorimetric MTT assay was used to measure the inhibition of snakegourd root polysaccharide on MCF-7 cells.The morphological changes of MCF-7 cells were observed by fluorescence microscope after DAPI staining and transmission electron microscope.The apoptosis of MCF-7 cells was examined by DNA agarose gel electrophoresis analysis of DNA fragmentation amd flow cytometry.The activity of Caspase-3 and Caspase-8 was detected by colorimetric assay.
Results: Polysaccharide of snakegourd root significantly inhibited MCF-7 cells in a dose- and time-dependent manner.The nuclear condensation and marginalization were observed by DAPI staining and transmission electron microscope.The characteristic ladder of apoptosis in DNA electrophoresis was detected in MCF-7 cells treated with 10.0 μmol/L polysaccharide of snakegourd root at d 2.The activities of Caspase-3 and Caspase-8 were increased in a time-dependent manner.The rates of apoptosis in MCF-7 cells were(5.2±1.3)%,(13.1±4.7)% ,(27.6±6.8)% and(43.8±9.8)% treated with 1.0,5.0,10.0 and 20.0 μmol/L snakegourd root polysaccharide at d 2,respectively.The maximal activities of intracellular Caspase-3 and Caspase-8 were(2.32±0.12)U/μg and(1.92±0.11)U/μg at d 2 and d 1,respectively when MCF-7 cells were treated with 10.0 μmol/L.
Conclusion: The polysaccharide of snakegourd root can induce the apoptosis of MCF-7 cells,which is associated with the activation of intracellular Caspase-3 and Caspase-8.
Diagnosis value of serum NKX2-1 for primary lung cancer
Journal of ZheJiang University(Medical Science), 2012, 41(5): 535-539.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.011
Abstract( 943 )   HTML (   PDF(639KB)( 314 )
Objective: To evaluate serum Nkx2-1 (NKX homeobox-1) levels in diagnosis of primary lung cancer.
Methods: The serum NKX2-1 and CEA (carcinoma embryonic antigen) levels were measured in 61 patients with primary lung cancer admitted from May 2009 to December 2010 and 49 healthy individuals served as controls.The receiver operating characteristic curve (ROC) of NKX2-1 in diagnosis for primary lung cancer was analyzed.The value of serum NKX2-1 in diagnosing primary lung cancer was compared with that of CEA by χ2 test and Kappa test.
Results: The serum Nkx2-1 levels in lung cancer were significantly higher than those in controls [(1.4206±0.1257)ng/ml vs (0.7646±0.0734)ng/ml,P<0.01].ROC analysis showed the area under the curve of serum NKX2-1 was 0.859.The Kappa value of NKX2-1 was higher than that of CEA (0.586 vs 0.396,P<0.05).Combination of serum NKX2-1 with CEA improved the Kappa value to 0.704,and also had high sensitivity (83.6%) and specificity (87.0%) for diagnosis of primary lung cancer.
Conclusions: Serum NKX2-1 protein can be used as a marker for diagnosis of lung cancer,the combination of NKX2-1 with CEA may further improve the diagnostic value.
Expression of RRM1 and ERCC1 genes in tumor tissues and peripheral blood lymphocytes of advanced non-small cell lung cancer
Journal of ZheJiang University(Medical Science), 2012, 41(5): 540-546.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.012
Abstract( 687 )   HTML (   PDF(1451KB)( 474 )
Objective: To investigate the expression of RRM1 and ERCC1 genes in tumor tissues and peripheral blood lymphocytes of advanced non-small cell lung cancer (NSCLC).
Methods: Tissue and peripheral blood samples were collected from 49 advanced NSCLC patients treated with gemcitabine plus carboplatin.The expressions of RRM1 and ERCC1 mRNA in tumor tissue and peripheral lymphocytes were detected by real-time fluorescent quantitative PCR.The relationship of gene expression with clinical characteristics,chemotherapy response and prognosis was analyzed.
Results: The RRM1 expression in tumor tissues was positively correlated with that in peripheral blood lymphocytes,while no significant correlation was observed between ERCC1 expression in tumor tissues and that in peripheral blood (rs=0.332 and 0.258;P=0.020 and 0.073,respectively).The expression of RRM1 and ERCC1 in tumor tissues peripheral lymphocytes was synchronous(rs=0.634 and 0.351;P<0.001 and 0.013,respectively).There was no significant correlation of gene expression with gender,age,smoking status,performance status,clinical stages and histological types of patients (P>0.05).Significant difference was found in response rate to chemotherapy(P<0.05,P<0.01,P<0.05),median survival time(P<0.05,P<0.01,P<0.05) and 1-year survival rate (P<0.01,<0.05,P<0.05) between patients with low RRM1 and ERCC1 expression levels in tumor tissues or low RRM1 expression levels in peripheral blood and those with high RRM1 and ERCC1 expression levels.The patients with low ERCC1 expression levels in tumor tissues gained higher 2-year survival rate(P<0.05).There was no correlation of the expression of ERCC1 in peripheral blood with the response to chemotherapy and prognosis(P>0.05).
Conclusion: The expression of RRMI and ERCC1 genes in tumor tissues and RRM1 in peripheral blood lymphocytes is closely correlated with the response to chemotherapy and prognosis of patients with advanced NSCLC treated with gemcitabine plus carboplatin.
Effect of Helicobacter pylori infection on platelet activation and coagulation function in patients with acute cerebral infarction
Journal of ZheJiang University(Medical Science), 2012, 41(5): 547-552.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.013
Abstract( 635 )   HTML (   PDF(727KB)( 283 )
Objective: To investigate the effect of Helicobacter pylori (Hp) on platelet activation and coagulation function in patients with acute cerebral infarction.
Methods: Sixty-six patients with acute cerebral infarction and 50 health individuals were enrolled in the study.Hp antibody,expression of CD62p on platelets and clotting indexes were measured and compared between two groups.
Results: The positive rate of Hp-IgG and Hp-CagA in cerebral infarction patients were higher than that in controls (P<0.05).The positive rate of CD62p in patients with positive Hp-IgG and Hp-CagA was significantly higher than that in negative patients and also controls (P<0.05).The APTT and TT were lower and FIB was higher in patients with positive Hp antibody than those in patients with negative Hp antibody(P<0.05),but there was no difference in PT,PTR and INR(P>0.05).
Conclusions: Hp infection can activate platelets and affect coagulation function,which may be involved in the development of cerebral infarction.
Sevoflurane preconditioning produces delayed cardioprotection effect through up-regulation of inducible nitric oxide synthase in rats
Journal of ZheJiang University(Medical Science), 2012, 41(5): 553-558.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.014
Abstract( 511 )   HTML (   PDF(758KB)( 320 )
Objective: To investigate whether inhaled svoflurane is capable of producing delayed cardioprotection effect in rats and its underlying mechanisms.
Methods: Male Sprague-Dawley rats inhaled 1.0 minimum alveolar concentration (MAC) sevoflurane,1.5 MAC sevoflurane,or O2 for 1 h.After 24 h and 48 h the left coronary artery of rats was occluded for 30 min,followed by 120 min of reperfusion.Hemodynamics was continuously recorded and myocardial infarct size was determined by Evans blue and triphenyltetrazolium chloride staining.The expression of nitric oxide synthase (NOS) was assessed by immunoblotting.
Results: 1.0 MAC sevoflurane and 1.5 MAC sevoflurane improved cardiac pump function after reperfusion and reduced myocardial infarct size with the increased iNOS expression(P<0.05).However,the expression of eNOS and p-eNOS was not affected(P>0.05).A selective iNOS inhibitor 1400 W abolished the cardioprotection effect induced by inhalation of 1.0 MAC sevoflurane for 24 h.
Conclusion: Sevoflurane produces delayed cardioprotection through the up-regulation of iNOS expression.
Effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats
Journal of ZheJiang University(Medical Science), 2012, 41(5): 559-563.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.015
Abstract( 506 )   HTML (   PDF(1038KB)( 319 )
Objective: To investigate the effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats.
Methods: Sprague-Dawley male rats were divided into 4 groups with 10 in each group:in S group rats received sham operation; in IR group rats were given with NS(1.0 ml/kg iv) 24 h before ischemia; in H group rats were treated with NaHS(0.05 mg/kg iv) 24 h before ischemia; and in D group,NaHS-treated rats received 5-hydroxydecanoate(5-HD) 15 min before ischemia. Rats in IR group,H group and D group were subjected to ischemia by occlusion of coronary artery for 30 min followed by 2 h of reperfusion.At the end of the reperfusion,myocardial infarct size was measured.SAM-s was measured by Western blotting.Plasma SOD activity and MDA were determined at the end of reperfusion.
Results: The infarct size was significantly lesser in H group (25.40%± 3.54%) than that in IR group (38.27%±5.64%,P<0.05).The SAM-s protein expression in myocardium was significantly lower in H group than that in IR group.The plasma MDA content was significantly lower and SOD activity was higher in H group than those in IR group,but there was no difference between IR group and D group.
Conclusion: The hydrogen sulfide preconditioning attenuates myocardial IR injury possibly through down-regulating SAM-s expression,reducing the production of oxygen free radicals and enhancing anti-oxidize effect in rats.
Corbrin shugan capsule for treatment of alcoholic hepatic fibrosis in rats
Journal of ZheJiang University(Medical Science), 2012, 41(5): 564-568.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.016
Abstract( 592 )   HTML (   PDF(1583KB)( 293 )
Objective: To investigate the therapeutic effect of Corbrin shugan capsule for treatment of alcoholic hepatic fibrosis in rats.
Methods: The rat model of alcoholic hepatic fibrosis was induced by intragastric administration of alcohol repeatedly. The serum procollagen Ⅲ(PC Ⅲ), laminin (LN) and tissue inhibitors of metalloproteinase-1 (TIMP-1) levels were measured with ELISA, and the content of hydroxyproline (Hyp) in liver tissue were determined with colorimetric method. Collagen deposition in liver tissue was observed with Masson′s staining, and the fibrosis area was measured with digital medical image analysis system (Motic Med 6.0).
Results: Compared with the model control group, the serum TIMP-1 and LN levels and hepatic fibrosis area in liver tissue significantly decreased in Corbrin shugan capsule groups with doses of 0.09,0.27 and 0.45 g·kg-1, and the serum PC Ⅲ and the Hyp contents in liver tissue also decreased of Corbrin shugan capsule groups with doses of 0.27 and 0.45g·kg-1.
Conclusion: Corbrin shugan capsule can decrease serum PC Ⅲ, TIMP-1 and LN levels and Hyp levels in liver tissue and hepatic fibrosis area in rats, indicating it may have therapeutic effect on alcoholic hepatic fibrosis.
Extraction and purification of acidic polysaccharide from Moerella iridescens
Journal of ZheJiang University(Medical Science), 2012, 41(5): 569-575.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.017
Abstract( 574 )   HTML (   PDF(1415KB)( 298 )
Objective: To optimize extraction and purification methods of acidic polysaccharide from Moerella iridescens (MIAP).
Methods: With alkali extraction process and orthogonal experiment,the time consumption,temperature,pH value of the solution and alcohol concentration during the extraction were optimized.The crude products were deprived of protein,pigment and ion,then were purified with DEAE-cellulose ion-exchange chromatography and verified with Sephadex G-100 and cellulose acetate membrane electrophoresis,and examined with infrared spectrum.
Results: The optimized extraction conditions were as follows:extraction time 6 h,extraction temperature 70℃,the solution pH 8.0 and the concentration of alcohol precipitation 70%.Intuitive features showed that the MIAP was pure white crystalline granular with slight dark brown color.The purification results demonstrated that the target MIAP was eluted and identified as a homogeneous components by DEAE-cellulose ion exchange column,Sephadex G-100 and cellulose acetate membrane electrophoresis.Infrared spectral scanning suggested that MIAP was α-D-type terminated glucopyranose.Intuitive features showed that MIAP was soft and cottony white.
Conclusion: The extraction process with orthogonal test has been optimized and the acidic polysaccharide from Moerella iridescens is successfully isolated.
Research advances on mechanisms of chemoresistance in acute myeloid leukemia
Journal of ZheJiang University(Medical Science), 2012, 41(5): 576-580.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.018
Abstract( 702 )   HTML (   PDF(509KB)( 348 )
Acute myeloid leukemia (AML) is a common type of hematopoietic malignancies seriously threatening human life.Resistance to chemotherapy is one of the main reasons for recurrence and refractoriness of acute myeloid leukemia.The mechanisms of chemoresistance are complex.This article reviews the mechanisms of chemoresistance in acute myeloid leukemia,the current research advances and the possible approach for reverse of drug resistance.
Research progress of protein tyrosine phosphatase SHP-2
Journal of ZheJiang University(Medical Science), 2012, 41(5): 581-585.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.019
Abstract( 787 )   HTML (   PDF(543KB)( 352 )
The Src homology-2 domain-containing phosphatase SHP-2 encoded by PTPN11 is an essential component in several signaling pathways.Different types of mutation in SHP-2 have been confirmed in several types of leukemia and solid tumors.Elucidation of the events underlying Shp2-evoked transformation may provide new insights into the novel targets for anti-cancer therapy.
Ubiquitination of recombinant adeno-associated viral vector and its application
Journal of ZheJiang University(Medical Science), 2012, 41(5): 586-591.   https://doi.org/10.3785/j.issn.1008-9292.2012.05.020
Abstract( 595 )   HTML (   PDF(837KB)( 339 )
Recombinant adeno-associated virus (rAAV) has been widely used as vector for gene therapy. However, the effectiveness of gene therapy based on rAAV needs to be further improved. Enhancement of the transduction efficiency is one of the most important fields for rAAV-based gene therapy. Recent results have showed that the ubiquitin-proteasome system plays an important role in the trafficking of rAAV vector in cytoplasm, and regulation of its function may significantly improve the transduction efficiency of rAAV vector in various types of cells and tissues.
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