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, Volume 40 Issue 4 Previous Issue    Next Issue
Current situation and future development in modernization of TCM
Journal of ZheJiang University(Medical Science), 2011, 40(4): 349-353.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.001
Abstract( 428 )   HTML (   PDF(1204KB)( 480 )
Marrow mesenchymal stem cell transplantation with sodium alginate gel for repair of spinal cord injury in mice
Journal of ZheJiang University(Medical Science), 2011, 40(4): 354-359.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.002
Abstract( 361 )   HTML (   PDF(2267KB)( 256 )
Objective: To investigate the effects of sodium alginate gels on marrow mesenchymal stem cell transplantation for repair of spinal cord injury (SCI) in mice.
Methods: In the present study,effects of different sterilization methods and concentrations of sodium alginate gels were examined. Marrow mesenchymal stem cells (mMSCs) were isolated from mice and cultured. Cells were cultured with sodium alginate gels and MTT assay was applied to determine the cell viability. Mice spinal cord injury was induced by spinal cord transection. mMSCs were transplanted into the cavity of injured spinal cord with sodium alginate gels. The effects of sodium alginate gel were assessed by BMS scales and immunofluorescence staining for NF-200.
Results: Compared with liquid form,solid form sodium alginate gel prepared with high pressure vapor sterilization had a better effect on cell viability. SCI mice treated with 10% sodium alginate gel and mMSCs achieved higher score in BMS scale as well as higher expression of NF-200 compared with the untreated SCI group.
Conclusion: Sodium alginate gel prepared with solid form sterilization induces mMSCs proliferation and thus can be used as the carrier of stem cell in treatment of SCI.
Effect of purple sweet potato on lipid metabolism and oxidative stress in hyperlipidemic rats
Journal of ZheJiang University(Medical Science), 2011, 40(4): 360-364.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.003
Abstract( 301 )   HTML (   PDF(1081KB)( 278 )
Objective: To investigate the effect of purple sweet potato on lipid metabolism and oxidative stress in hyperlipidemic rats.
Methods: Forty male SD rats were randomly divided into 4 groups: normal control group,high-fat control group,high purple sweet potato groups,low purple sweet potato group. The rats were fed with different diets for 6w respectively.
Results: Serum TC,TG levels were significantly lower in high dosage group than in high-fat control group; while only serum TG was significantly lower in low dosage group than in high-fat control group,these changes started at the third week and lasted to the end of experiment. Serum LDL-C and AI levels were significantly lower in high and low dosage group than in high-fat control group,whereas,serum HDL-C was significantly higher than that in high-fat control group at w3 and lasted to the end of experiment. Serum SOD was significantly higher in high and low dosage group than in high-fat control group,whereas,serum MDA was significantly lower than that in high-fat control group at w6.
Conclusion: Purple sweet potato can decrease serum lipids and reduce hepatic oxidative stress in hyperlipidemic rats.

Anti-tumor activity of components isolated from purple sweet potato polysaccharides
Journal of ZheJiang University(Medical Science), 2011, 40(4): 365-373.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.004
Abstract( 346 )   HTML (   PDF(3081KB)( 422 )
Objective: To isolate and purify components from polysaccharides of purple sweet potato (PPSP) and to test their anti-tumor activity.
Methods: DEAE-Cellulose and CM-Cellulose exchange chromatography were applied to separate components of PPSP. The anti-tumor activities of each component were measured by MTT assay on Hela and HepG2 cells and their monosaccharide composition were analyzed by TLC chromatography,followed by infrared spectroscopy studies.
Results: Through weak anion exchange chromatography and gradient elution by sodium chloride solution,four components were separated and named as PPSP,PPSPⅡ,PPSPⅢ and PPSPⅣ,respectively. MTT tests showed that PPSPⅡ and PPSPⅢ inhibited Hela and HepG2 tumor cells in a certain extent. The structural analysis revealed that PPSPI was mainly composed of glucose and galactose,PPSPⅡwas composed of glucose and had a typical absorption peak of β-D-glucose chitosan pyranose,PPSPⅢ was a glycoprotein showing a protein absorption peak.
Conclusion: Four components were separated from PPSP successfully,among which PPSPⅡ and PPSP Ⅲ shows anti-tumor activities on Hela and HepG2 cells in vitro.

Effect of purple sweet potato flavonids on metabolism of glucose and lipids in diabetic rats
Journal of ZheJiang University(Medical Science), 2011, 40(4): 374-379.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.005
Abstract( 320 )   HTML (   PDF(1582KB)( 323 )
Objective: To investigate the effects of purple sweet potato flavonoids (PSPF) on blood glucose and lipids levels in diabetic rats.
Methods: Diabetes was induced by intraperitoneal injection of streptozotocin (STZ,65 mg.kg-1) in rats. The changes of fasting blood glucose and lipids levels in serum and body weight,food and fluid intake of diabetic rats treated with PSPF were examined.
Results: Diabetic symptoms were ameliorated after rats were fed with PSPF. The fasting blood glucose (FBG),GSP,TC,TG,LDL-C were decreased and serum HDL-C levels were increased(P<0.01) in high,medium dose PSPF groups; while FBG,serum GSP,TG,LDL-C were also improved in low dose group(P<0.05 or P<0.01).
Conclusion: Purple sweet potato flavonoids can decrease the blood glucose and lipids levels in diabetic rats.
Components of myrsinane-type diterpenes from Euphorbia prolifera
Journal of ZheJiang University(Medical Science), 2011, 40(4): 380-383.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.006
Abstract( 305 )   HTML (   PDF(1293KB)( 245 )
Objective: To extract and isolate the component from myrsinane-type diterpenes of Euphorbia prolifera.
Methods: Petroleum extraction and chromatography on the silica gel were used to extract and isolate the diterpenes of Euphorbia prolifera.
Results: Eight components of myrsinol diterpenes were isolated,namely: Proliferin A (1),Proliferin B (2),Proliferin C (3),Proliferin D (4),Euphorprolitherin B (5),Euphorprolitherin D (6),SPr5 (7) and 14-desoxo-3-O-prorionyl-5,15-di-O-acetyl-7-O-nicotinoyl-myrsinol-14β-acetate (8). Their structures were identified with mass-spectroscopic methods and NMR techniques. The cytotoxicity of compounds 1,2,and 4 against cancer cells was evaluated,with compound 1 being active against A2780 cancer cells (IC50 7.7 μmol/L).
Conclusion: Myrsinane-type diterpene Proliferin A from Euphorbia prolifera shows cytotoxic effect against human ovarian cancer cell line A2780.
Comparison of behavioral effects of psychoactive drugs between two strains of mice
Journal of ZheJiang University(Medical Science), 2011, 40(4): 384-390.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.007
Abstract( 385 )   HTML (   PDF(2089KB)( 269 )
Objective: To compare the behavioral effects of psychoactive drugs between two strains of mice.
Methods: The Kunming (KM) and ICR mice were injected intraperitonealy with caffeine (3,10,30,100 mg/kg),ephedrine (3,10,30,100 mg/kg),diazepam (1,3,10 mg/kg) and chloral hydrate (10,30,100 mg/kg),respectively. Ten min after injection,the locomotor activity in the open field was recorded for 2 h. The total distance,the distance ratio to total distance and the time in central region were analyzed for each drugs. Thirty min after injection,the latent time in the passive avoidance test was measured in a shuttle box.
Results: Caffeine and diazepam prolonged the latent time,and ephedrine and chloral hydrate decreased the latent time,but there were no differences between the two strains. The two strains of mice exhibited significant differences in the total distance after injection of ephedrine 10 mg/kg,diazepam 3 mg/kg and chloral hydrate 100 mg/kg. Compared to KM mice,ICR mice exhibited an increase in the distance ratio and the time in central region after injection of ephedrine 10-100 mg/kg,but a decrease after diazepam 3-10 mg/kg.
Conclusion: KM and ICR mice show no differences in latent time,but significant differences in the total distance,the distance ratio and the time in central region in the locomotor activity. Therefore,selection of mouse strains is important in the study of psychoactive drugs.
Protective effect of Jiangbaiweiyan tablet on ethanol-induced gastric mucosa injury in rats
Journal of ZheJiang University(Medical Science), 2011, 40(4): 391-394.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.008
Abstract( 369 )   HTML (   PDF(1071KB)( 271 )
Objective: To investigate the effect of Jiangbaiweiyan tablet,a Chinese medicine compound composed of Alpinta Officinarum,Cyperus Rotundus,Bulbus Lilii and Rlindera Strychnifolia,on ethanol-induced gastric mucosa injury in rats.
Methods: Acute gastric ulcer was induced in rats with absolute ethyl alcohol. The ulcer index was used to evaluate the extent of the gastric mucosa injury.
Results: The ulcer indexes of the model group,the mid-dose(1.08 g·kg-1·d-1)and high-dose(2.16 g·kg-1·d-1)of Jiangbaiweiyan tablet groups were 141.58±47.43,24.83±23.04 and 2.12±2.58,respectively (P﹤0.01).
Conclusions: Jiangbaiweiyan tablet has protective effects on ethanol-induced gastric mucosa injury in rats,which may be related to anti-oxidation and enhancing tissue regeneration capacity.
Study on determination and pharmacokinetics of metabolites from Folium Mori extract in rats
Journal of ZheJiang University(Medical Science), 2011, 40(4): 395-401.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.009
Abstract( 357 )   HTML (   PDF(1689KB)( 243 )
Objective: To establish a RP-HPLC method for simultaneous determination of total quercetin,kaempferol and isorhamnetin in rat plasma after oral administration of Folium Mori extract (FME).
Methods: After a single dose of FME (110 mg/kg) was taken,rat plasma samples were collected. The samples were hydrolyzed with hydrochloric acid (c=3.0 mol/L),the mixed solution was extracted with etheracetone mixture. The total quercetin,kaempferol and isorhamnetin in plasma samples were determined by HPLC,pharmacokinetic parameters were calculated by DAS 3.0 software.
Results: The method was linear over the concentration ranges of 0.0545-8.70,0.0954-14.7 and 0.0545-8.55 μg/ml for quercetin,kaempferol and isorhamnetin,respectively (r=0.9979,0.9993,0.9981). The absolute recoveries were 85.3%-86.1%,79.4%-86.7% and 62.8%-89.7%,respectively and the assay recoveries were all from 94.7% to 107%. The relative standard deviation (RSD) of intra-and inter-day were less than 9.5% and 9.8%,respectively. The main pharmacokinetic parameters were as follows: T1/2z was 92.7,67.9 and 54.2 h; Tmax was 0.400,0.400 and 3.87 h; AUC(0-∞) was 68.0,67.5 and 32.8 mg/h/L; MRT(0-∞) was 128,85.2 and 72.0 h for quercetin,kaempferol and isorhamnetin,respectively.
Conclusions: The method established in this study is accurate,reliable and reproducible,and can be applied for determination of total quercetin,kaempferol and isorhamnetin in rat plasma after oral administration of FME; the pharmacokinetic studies showed that the distribution of drugs is rapid and elimination is very slow.
Inhibitive effect of C-21 steroidal glycosides of Cynanchum auriculatum on rat glioma cells in vitro
Journal of ZheJiang University(Medical Science), 2011, 40(4): 402-407.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.010
Abstract( 343 )   HTML (   PDF(2823KB)( 239 )
Objective: To evaluate the inhibitive effect of C-21 steroidal glycosides from the root of Cynanchum auriculatum (CGB) on rat glioma C6 cells.
Methods: C6 cells were treated with CGB for 24,48,72 h at concentration of 30,60,120 mg/L,respectively. MTT assay was used for evaluating cell viability; fluorescence-activated cell sorting analysis after Annexin V/propidium iodide staining or single propidium iodide staining was used to test cell apoptosis and cell cycle.
Results: CGB at 30,60,120 mg/L concentration-dependently decreased C6 cell viability (P<0.001). CGB at 60 and 120 mg/L induced C6 cell apoptosis and cell cycle arrest. The fraction of G0/G1 cells was increased (P<0.05) and that of S phase cells was decreased (P<0.01).
Conclusion: CGB can inhibit the growth of rat glioma C6 cells,and induce apoptosis and G0/G1 cell cycle arrest.
Comparison on antitumor activity of cisplatin-loaded liposomes and nanoparticles in vitro
Journal of ZheJiang University(Medical Science), 2011, 40(4): 408-413.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.011
Abstract( 368 )   HTML (   PDF(1958KB)( 259 )
Objective: To compare the differences in antitumor activity between cisplatin (CDDP)-loaded liposomes and nanoparticles in vitro.
Methods: CDDP-gelatin nanoparticles (GPs-Pt) and CDDP-liposomes with similar size,zeta potential,drug loading efficiency and in vitro release property were prepared. The uptake in A549 cells and elimination kinetics were evaluated and antitumor activity was determined by MTT test. The internalization pathways of nanocarriers were studied with inhibitors.
Results: Internalization of two nanocarriers was clathrin and actin dependent. Pt accumulation delivered by GPs-Pt was significantly higher than that of liposomes. However,the results of kinetic analysis showed that liposomes had longer cellular retention,and the MRT and AUC were 3 times and twice of GPs-Pt,respectively. The IC50 of liposomes was significantly lower than GPs-Pt. The values were 2.94±0.21 and 20.70±1.05 μg/ml,respectively.
Conclusions: Nanocarriers with similar pharmaceutical parameters can induce differences in cellular internalization and elimination,which influence the antitumor activity eventually. Compared with gelatin nanoparticle,liposome is preferable for cispaltin delivery.
Porphyrinated polyimide nanofibrous membrane for rapid detection of trace amount of methanol vapor
Journal of ZheJiang University(Medical Science), 2011, 40(4): 414-420.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.012
Abstract( 318 )   HTML (   PDF(3526KB)( 253 )
Objective: To prepare novel electrospun porphyrinated polyimide nanofibrous membrane for rapid detection of trace amount of methanol vapor.
Methods: Porphyrin chromophore was copolyimized into polyimide backbones and the porphyrinated polyimide nanofibrous membrane was prepared by electrospinning technique. By optimizing the processing parameters,such as solution concentration and electrospinning voltage,nanofibrous membrane with three dimensional and large surface-to-area ratio structure was fabricated for trace amount of methanol vapor sensing applications.
Results: The obtained nanofibrous membrane preserved typical photophysical properties of porphyrin chromophores with uniformly fine and smooth fiber diameter. When exposed to methanol vapor,a red-shift of the absorption spectra and decrease in the emission intensities was observed,while no significant changes were seen when the membrane contacting with other common alcohols. After five times of 150 ppm methanol vapor quenching and nitrogen gas regeneration,the fluorescence of the membrane remained unchanged,indicating a good reversibility.
Conclusions: Combining the specific optical properties of porphyrin with large surface-area-to-volume ratio of nanofibrous membranes,a porphyrinated polyimide nanofibrous membrane has been facilely fabricated for trace methanol vapor detection. The sensing membrane exhibits good sensitivity,selectivity and reproducibility.
Association of thrombospondin-1 gene N700S polymorphism with coronary artery disease
Journal of ZheJiang University(Medical Science), 2011, 40(4): 421-426.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.013
Abstract( 336 )   HTML (   PDF(1240KB)( 232 )
Objective: To investigate the association of thrombospondin -1 (TSP-1) gene A8831G (N700S) polymorphism with coronary artery disease (CAD).
Methods: This study was conducted with a case-control design including 178 patients with CAD (55 AMI) and 158 healthy subjects.The TSP-1 N700S polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism analysis.
Results: No significant difference of the AG genotype in CAD group and control group (1.7% vs 0.6%,P=0.375) was detected. None of the homozygotes was detected for the G allele. The prevalence of the G allele was not significantly different between CAD group and controls (0.8% vs 0.3%,P=0.376). No significant difference of the AG genotype in AMI group and control group (3.6% vs 0.6%, P=0.104). The prevalence of G allele was not significantly different between AMI patients and controls (1.8% vs 0.3%,P=0.364).
Conclusion: There are TSP-1 N700S polymorphisms in Chinese Zhejiang Han people,but the TSP-1 N700S variant shows a much lower prevalence compared with Western populations and may be not a potential risk for CAD and AMI.
Establishment of NCAM L1 minigene model and its splicing patterns in different cell lines
Journal of ZheJiang University(Medical Science), 2011, 40(4): 427-431.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.014
Abstract( 414 )   HTML (   PDF(1216KB)( 237 )
Objective: To establish a minigene model of neural cell adhesion molecule L1 (NCAM L1)gene and to study its aplicing patterns in different cell lines.
Methods: Using human genetic cDNA as template,the NCAM L1 minigene fragment was amplified and inserted into eukaryotic expression vector. The minigene was transfected into 4 cell lines and the splicing patterns of NCAM L1 minigene in these cell lines were studied.
Results: The splicing patterns of NCAM L1 minigene were different in individual cell lines.In PFSK and Hela cell lines,two splicied isoforms were generated but in COS-1 and R28 cell lines,only one isoform existed.
Conclusion: NCAM L1 minigene model can be used in alternative splicing analysis.
Application of digital photography in color matching in dentistry
Journal of ZheJiang University(Medical Science), 2011, 40(4): 432-435.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.015
Abstract( 488 )   HTML (   PDF(1299KB)( 240 )
Objective: To develop a new color matching method in dentistry by application of digital photography.
Methods: Digital photographs were obtained of Vitapan 3D-Master shade guide and natural teeth under the same condition,the L*a*b* values of each digital photography were assessed and analyzed by Photoshop CS4.
Results: The Vitapan 3D-Master shade guide was divided into 5 groups,the L* values were similar in each group,but decreased from group 1 to 5. The a* values of L1.5 and L2.5 were minimum,R1.5 and R2.5 were maximum and M1-M3 were intermediate. Compared with Vitapan 3D-Master shade guide,the L*a*b* values of natural teeth were higher.
Conclusion: Digital photography can basically reflect the color of Vitapan 3D-Master shade guide,and provides a reference for color matching in dentistry.
Evaluation of different methods in determination of low level HBsAg
Journal of ZheJiang University(Medical Science), 2011, 40(4): 436-439.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.016
Abstract( 342 )   HTML (   PDF(1074KB)( 382 )
Objective: To evaluate chemiluminescent immunoassay (CLIA),electrochemiluminescent immunoassay (ECLIA) and ELISA in determination of low level HBsAg.
Methods: According to the standard of CLIA Architect i2000,70 samples were divided into three groups by HBsAg concentration : ﹤1 ng/ml,1-5 ng/ml and ≥5 ng/ml.The samples were also determined by ECLIA MODULAR<E>170 and ELISA,and the results were compared with those measured by CLIA Architect i2000.
Results: The concordance rates of ECLIA MODULAR<E>170 with Architect i2000 was 79.2% for﹤1 ng/ml group,100% for 1-5 ng/ml group,100% for ≥5 ng/ml group. And the concordance rates of ELISA with Architect i2000 was 0% for﹤1 ng/ml group,45.5% for 1-5 ng/ml group and 100% for ≥5 ng/ml group.
Conclusion: For determination of low level HBsAg,CLIA Architect i2000 and ECLIA MODULAR<E>170 have high credibility. ELISA is also credible when HBsAg ≥5 ng/ml,but not for low level HBsAg,especially for HBsAg﹤1 ng/ml.
Mechanism of NMDA receptor-dependent neuronal survival and neuroprotection
Journal of ZheJiang University(Medical Science), 2011, 40(4): 440-445.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.017
Abstract( 427 )   HTML (   PDF(1087KB)( 244 )
NMDA (N-methyl-D-aspartate) receptors are a subtype of glutamate receptor,and play an important role in both synaptic transmission and synaptic plasticity. When excessively excited,NMDA receptors can cause cell death in many neuropathological scenarios such as OGD (oxygen and glucose deprivation) and brain injury. However,recent studies have revealed that under the physical and some pathological conditions,NMDA receptor could promote neuronal survival and protect neurons from damage.
Research progress on spindle assembly checkpoint gene BubR1
Journal of ZheJiang University(Medical Science), 2011, 40(4): 446-450.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.018
Abstract( 363 )   HTML (   PDF(1146KB)( 266 )
BubR1 gene is a homologue of the mitotic checkpoint gene Mad3 in budding yeast which is highly conserved in mammalian. BubR1 protein is a key component mediating spindle assembly checkpoint activation. BubR1 safeguards accurate chromosome segregation during cell division by monitoring kinetochore-microtubule attachments and kinetochore tension. There is a dose-dependent effect between the level of BubR1 expression and the function of spindle assembly checkpoint. BubR1-deficient would lead to mitotic progression with compromised spindle assembly checkpoint because cells become progressively aneuploid. Recently,it has been reported that BubR1 also plays important roles in meiotic,DNA damage response,cancer,infertility,and early aging. This review briefly summarizes the current progresses in studies of BubR1 function.
Current progress in research on epigenetic effects of air pollution
Journal of ZheJiang University(Medical Science), 2011, 40(4): 451-457.   https://doi.org/10.3785/j.issn.1008-9292.2011.04.019
Abstract( 370 )   HTML (   PDF(1105KB)( 325 )
Air pollution is associated with numerous diseases. In recent years,researches have increasingly showed that epigenetic modifications usually occur at the early stage of diseases,and make greater contributions to the occurrence and development of diseases compared to genetic abnormalities. Thus,researches on epigenetic effects of air pollution would serve for better understanding the interaction between air pollutants and genome in the pathogenesis of disease. Meanwhile,in order to reduce the exposure to air pollution and diminish the adverse effects related,further studies are needed to identify epigenetic biomarkers of air pollution so that we can take timely and effective measures in disease prevention.
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