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Journal of ZheJiang University(Medical Science)  1999, Vol. 28 Issue (2): 59-61    DOI: 10.3785/j.issn.1008-9292.1999.02.005
The Ultrastructural Observation of Three Dimensional Culture of Fibroblasts
Han Chunmao
Department of Burns, Second Affiliated Hospital of Zhejiang Medical University
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Objective:To develop a three dimensional fibroblast culture method for wound healing studies.Methods:By using the permeable collagen membrane as a substrate,we modified the three-dimensional fibroblast culture.After being cultured for 6 weeks,the fibroblast cultures were double colored with uranium acetate and lead nitrate and finally observed with electron microscopy.Results: Besides the multilayer,dense extracellular matrix and myofibroblasts in the cultrue,myofibroblasts were also found in the lower layer of the culture.Conclusion:The fibroblast culture model can be used in the study of wound healing,especially of the hypertrophic scar and keloid.

Key wordsFibroblasts/ultrastruct      Cell cultured     
Received: 27 October 1997      Published: 25 March 1999
Cite this article:

Han Chunmao. The Ultrastructural Observation of Three Dimensional Culture of Fibroblasts. Journal of ZheJiang University(Medical Science), 1999, 28(2): 59-61.

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关键词: 纤维母细胞/超威结构,  细胞的培养 
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