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Journal of ZheJiang University(Medical Science)  1999, Vol. 28 Issue (2): 59-61    DOI: 10.3785/j.issn.1008-9292.1999.02.005
    
The Ultrastructural Observation of Three Dimensional Culture of Fibroblasts
Han Chunmao
Department of Burns, Second Affiliated Hospital of Zhejiang Medical University
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Abstract  

Objective:To develop a three dimensional fibroblast culture method for wound healing studies.Methods:By using the permeable collagen membrane as a substrate,we modified the three-dimensional fibroblast culture.After being cultured for 6 weeks,the fibroblast cultures were double colored with uranium acetate and lead nitrate and finally observed with electron microscopy.Results: Besides the multilayer,dense extracellular matrix and myofibroblasts in the cultrue,myofibroblasts were also found in the lower layer of the culture.Conclusion:The fibroblast culture model can be used in the study of wound healing,especially of the hypertrophic scar and keloid.



Key wordsFibroblasts/ultrastruct      Cell cultured     
Received: 27 October 1997      Published: 25 March 1999
Cite this article:

Han Chunmao. The Ultrastructural Observation of Three Dimensional Culture of Fibroblasts. Journal of ZheJiang University(Medical Science), 1999, 28(2): 59-61.

URL:

https://www.zjujournals.com/med/10.3785/j.issn.1008-9292.1999.02.005     OR     https://www.zjujournals.com/med/Y1999/V28/I2/59


成纤维细胞三维培养物的超威结构

目的:研究成纤维细胞在疤痕形成中的作用。方法:采用可渗性胶原膜(Celgen®)作为底物,改进成纤维细胞的三维培养,6周培养后的培养物用醋酸铀和硝酸铅双重染色后用电镜进行观察。结果:发现培养物不仅有多层化结构、细胞外间质和肌成纤维细胞,而且培养物底层有肌成纤维细胞和大量胶原。结论:本文所揭示的成纤维细胞培养模型有利于创伤愈合,尤其是增生性疤痕和疤痕疙瘩的研究。


关键词: 纤维母细胞/超威结构,  细胞的培养 
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