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Journal of ZheJiang University(Medical Science)  2011, Vol. 40 Issue (1): 17-22    DOI: 10.3785/j.issn.1008-9292.2011.01.004
    
Comprehensive quality control method for total flavonoid of Fructus Aurantii
LUAN Lian-jun,JIANG Zhou-hong,MI Song-yuan,WU Yong-jiang
Department of Chinese Materia Medica,Zhejiang University,Hangzhou 310058,China
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Abstract  Objective: To establish a comprehensive quality control method for total flavonoid of Fructus Aurantii.
Methods: RP-HPLC and spectrophotometry were applied for the quantitative and fingerprint analysis of total flavonoid of Fructus Aurantii. The contents of naringin and neohesperidin were determined on an Agilent SB-C18 column (4.6 mm×250 mm,5 μm). The mobile phase was composed of 0.02% H3PO4 and CH3CN(80∶20). The flow rate was 1 ml/min with DAD detected at 280 nm. The column temperature was maintained at 35℃. The fingerprints were developed on an Agilent SB-C18 column(4.6 mm×250 mm,5 μm). The mobile phase was composed of 0.5% HAc and CH3OH with a linear gradient elution. The ratio of 0.5% HAc and CH3OH was: 0 min,80∶20; 10 min,60∶40; 35 min,30∶70; 50 min,0∶100. The flow rate was 1 ml/min with DAD detected at 320 nm. The column temperature was maintained at 30℃. Meanwhile,the contents of total flavonoid were determined at 283 nm.
Results: The contents range of naringin,neohesperidin and total flavonoid were 38.3%-47.2%,21.0%-28.5% and 79.9%-88.6%,respectively. The fingerprints of the effective fractions showed 12 common peaks and the fingerprint similarity was all above 98.0% compared with the standard chromatogram.
Conclusion: The method reported in this paper can be used effectively for the quality control of total flavonoid of Fructus Aurantii.


Key wordsFRUCTUS AURANTII/chem;       Chromatography, high pressure liquid/methods; Quality control;       Flavones/anal; Nucleotide mapping     
Published: 25 January 2011
Cite this article:

. Comprehensive quality control method for total flavonoid of Fructus Aurantii. Journal of ZheJiang University(Medical Science), 2011, 40(1): 17-22.

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https://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2011.01.004     OR     https://www.zjujournals.com/med/Y2011/V40/I1/17


枳壳总黄酮的多指标质量控制

目的:建立枳壳总黄酮多指标质量控制方法。
方法:采用高效液相色谱法测定枳壳总黄酮中有效成分含量和指纹图谱,紫外分光光度法测定总黄酮含量。含量测定色谱条件为:Agilent SB-C18色谱柱(4.6 mm×250 mm,5 μm),流动相为0.02%磷酸水溶液-乙腈(80∶20),流速1 ml/min,柱温35℃,检测波长280 nm。指纹图谱条件为:Agilent SB-C18色谱柱(4.6 mm×250 mm,5 μm),流动相A(0.5%醋酸水溶液,V/V)、B(甲醇);梯度洗脱程序(min、B%):0 min、20%,10 min、40%,35 min、70%,50 min、100%;流速1 ml/min,柱温30℃,检测波长320 nm。总黄酮检测波长为283 nm。
结果:经方法学认证,含量测定和指纹图谱分析各项参数均符合要求;10批枳壳总黄酮样品的含量分析显示:柚皮苷、新橙皮苷含量为38.3%~47.2%及21.0%~28.5%,总黄酮含量79.9%~88.6%;各批次样品指纹图谱分析都显示12个共有峰,与标准谱图比较,指纹图谱相似度均在98.0%以上。
结论:指纹图谱与关键成分含量测定相结合的分析方法能很好地反映枳壳总黄酮整体质量,是一种有效的质量分析与评价方法。该方法测定结果准确,重复性好,可有效控制枳壳总黄酮质量。

关键词: 枳壳/化学,  色谱法,  高压液相/方法,  质量控制,  黄酮类/分析,  核苷酸图谱 
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