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Journal of ZheJiang University(Medical Science)  2011, Vol. 40 Issue (4): 402-407    DOI: 10.3785/j.issn.1008-9292.2011.04.010
    
Inhibitive effect of C-21 steroidal glycosides of Cynanchum auriculatum on rat glioma cells in vitro
WANG Yi-qi1,YANG Bo1,ZHANG Ru-song1,WEI Er-Qing2
1.College of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 310053,China; 2.Department of Pharmacology,College of Medicine,Zhejiang University,Hangzhou 310058,China
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Abstract  Objective: To evaluate the inhibitive effect of C-21 steroidal glycosides from the root of Cynanchum auriculatum (CGB) on rat glioma C6 cells.
Methods: C6 cells were treated with CGB for 24,48,72 h at concentration of 30,60,120 mg/L,respectively. MTT assay was used for evaluating cell viability; fluorescence-activated cell sorting analysis after Annexin V/propidium iodide staining or single propidium iodide staining was used to test cell apoptosis and cell cycle.
Results: CGB at 30,60,120 mg/L concentration-dependently decreased C6 cell viability (P<0.001). CGB at 60 and 120 mg/L induced C6 cell apoptosis and cell cycle arrest. The fraction of G0/G1 cells was increased (P<0.05) and that of S phase cells was decreased (P<0.01).
Conclusion: CGB can inhibit the growth of rat glioma C6 cells,and induce apoptosis and G0/G1 cell cycle arrest.


Key wordsPolygonium multiflorum/chem      Apoptosis/drug eff      Cynanchum auriculatum      C-21 Steroidal Glycosides      Glioma      Apoptosis      cell cycle     
Published: 25 July 2010
Cite this article:

. Inhibitive effect of C-21 steroidal glycosides of Cynanchum auriculatum on rat glioma cells in vitro. Journal of ZheJiang University(Medical Science), 2011, 40(4): 402-407.

URL:

https://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2011.04.010     OR     https://www.zjujournals.com/med/Y2011/V40/I4/402


白首乌C-21甾体苷体外抑制大鼠胶质瘤细胞生长的作用

目的:评价白首乌C-21甾体苷(CGB)体外抑制大鼠胶质瘤C6细胞生长的作用。
方法:C6细胞在CGB处理24、48、72 h后,以MTT法检测细胞活性;AnnexinV/PI 双染或用PI单染后,流式细胞术检测细胞凋亡及细胞周期。
结果:CGB 30、60、120 mg/L显著抑制C6细胞活性(P<0.001),呈浓度、时间依赖性;CGB 60、120 mg/L显著提高细胞凋亡率(P<0.001),并能明显阻滞细胞周期,增加G0/G1期细胞比例(P<0.05),降低S期细胞比例(P<0.01)。
结论:CGB能显著抑制体外培养的大鼠胶质瘤细胞的生长,并能诱导细胞凋亡,阻滞细胞周期。

关键词: 何首乌/化学,  细胞凋亡/药物作用,  白首乌,  C-21甾体苷,  胶质瘤,  凋亡,   ,  细胞周期 
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