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| PP2 enhances intercellular communication of gap junction in breast cancer Hs578T cells |
| DONG Shu-Ying, ZHENG Chao, JIANG Guo-Jun, HAN Xi, TONG Xu-Hui |
| Department of Pharmacology，Faculty of Pharmacy，Bengbu Medical College，Bengbu 233000，China |
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Abstract Objective： To investigate the effect of Src kinase inhibitor PP2 on intercellular communication of gap junction in breast cancer cells.
Methods： Cultured breast cancer Hs578T cells were treated with various concentrations of pp2 (0，1，2，4，8，16，32 μmol/L) for 24h.Cell growth was determined by MTT assay; dye spread in Hs578T cells was measured by Parachute assay; and the expression of Src kinase in Hs578T cells was detected by Western blot.
Results： MTT assay showed that the survive rate of Hs578T cells treated with PP2 (1~8 μmol/L) was 98%±3%~94%±4%.Parachute assay showed that compared to control group the standard normalized dye spread rates of Hs578T cells treated with 1，2，4 and 8 μmol/L PP2 were 1.60±0.08，2.00±0.05，2.20±0.05 and 2.70±0.09，respectively (all P<0.01).Moreover，compared to control group at the same time points，the standard normalized dye spread of Hs578T cells treated with 8 μmol/L PP2 for 6，12 and 24 h were 1.4±0.05，1.7±0.06，and 2.2±0.07，respectively (all P<0.01).Western blot showed that the expression ratios of Src kinase/β-actin of Hs578T cells treated with 1，2，4 and 8 μmol/L PP2 for 24 h were 0.93±0.02，0.70±0.09，0.66±0.09 and 0.36±0.10，which were significantly inhibited compared with control group (P<0.05 or 0.01).And the expression ratio of Src kinase/β-actin of Hs578T cells treated with 8 μmol/L PP2 for 6，12 and 24h was 0.82±0.03，0.66±0.08 and 0.59±0.09，which were all inhibited significantly compared to control group (P<0.01).
Conclusion： PP2 enhances the gap junction function in breast cancer Hs578T cells，which is probably related to the inhibition of Src kinase.
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Received: 17 December 2012
Published: 25 September 2013
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PP2可增强乳腺癌Hs578T细胞缝隙连接功能
目的：探讨Src激酶抑制剂PP2对乳腺癌细胞Hs578T缝隙连接功能的影响。
方法：MTT法检测PP2对乳腺癌细胞生长的影响；细胞接种荧光示踪法观察PP2对乳腺癌细胞之间荧光传递功能的影响；Western blot法检测PP2对乳腺癌细胞Src激酶表达的影响。
结果：1~8 μmol/L浓度的PP2对乳腺癌细胞生长几乎无影响；细胞接种荧光示踪结果显示，1~8 μmol/L的PP2能显著增强细胞荧光传递功能（P<0.01），8 μmol/L的PP2作用6、12和24 h后细胞荧光传递功能亦明显增强（P<0.01）；Western blot结果显示，PP2(1~8 μmol/L)可显著降低Src激酶表达（P<0.05~0.01），8 μmol/L PP2作用6、12和24 h后Src激酶的表达亦明显降低（P<0.01）。
结论：PP2能增强乳腺癌细胞Hs578T的缝隙连接功能，这种增强作用可能与其降低Src激酶表达有关。
关键词：
乳腺肿瘤,
src族激酶类/拮抗剂和抑制剂,
连接蛋白类,
肿瘤细胞, 培养的
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