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Journal of ZheJiang University(Medical Science)  2015, Vol. 44 Issue (3): 329-334    DOI: 10.3785/j.issn.1008-9292.2015.05.14
Expression of S100A8 and A100A9 in giant cell tumor of bone and its relation with CT and MR imaging findings
LIAO Jin-sheng1,2, DING Xiao-yi2, XU Shun-liang1
1. Department of Radiology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;
2. Department of Radiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
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Objective: To investigate the mRNA and protein expression levels of S100A8 and S100A9 in giant cell tumor(GCT) of bone,and its relation with radiological findings and biological behavior. Methods: Forty three patient with GCT of bone admitted in Ruijin Hospital Shanghai Jiaotong University School of Medicine from January 2009 to June 2012 were enrolled in the study. The expression levels of S100A8 and S100A9 mRNA and protein were detected by using semiquantitative RT-PCR and Western blotting in 43 specimens of GCT and 6 specimens of normal bone marrow. The CT and MRI findings of patients were retrospectively reviewed, its relation with tissue expression of S100A8 and S100A9 was analyzed. Results: Among 43 GCT cases 40 showed positive expression of S100A8 and S100A9 mRNA and protein, and the expression levels were significantly higher than those in normal bone marrow(P<0.05). The expression level of S100A8 protein was significantly different in bone GCT with different composition ratio on MRI(P<0.05).The expression level of S100A9 protein was significantly different in GCT with different degree of bone destruction on CT scan(P<0.05). Conclusion: The expression of S100A8 and S100A9 mRNA and protein is up-regulated in GCT of bone. The expression of S100A8 and S100A9 is associated with the real composition ratio and the degree of bone destruction, respectively, indicating that S100A8 and S100A9 may be involved in the biological behavior of bone GCT.

Key wordsGiant cell tumor of bone/pathology      Giant cell tumor of bone/radiography      RNA, messenger/genetics      S100 proteins/biosynthesis      Tomography, X-ray computed      Magnetic resonance imagine     
Received: 23 November 2014      Published: 25 May 2015
CLC:  R738.1  
Cite this article:

LIAO Jin-sheng, DING Xiao-yi, XU Shun-liang. Expression of S100A8 and A100A9 in giant cell tumor of bone and its relation with CT and MR imaging findings. Journal of ZheJiang University(Medical Science), 2015, 44(3): 329-334.

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目的:检测骨巨细胞瘤患者肿瘤组织中S100A8、S100A9 mRNA及蛋白的表达水平,探讨其与骨巨细胞瘤的影像学及生物学行为之间的关系。方法:收集2009年1月至2012年6月在上海交通大学医学院附属瑞金医院诊治的43例骨巨细胞瘤患者的临床资料,并采集骨巨细胞瘤新鲜组织和6例正常成人股骨的骨髓组织,运用半定量逆转录PCR及蛋白质印迹法检测肿瘤组织中S100A8、S100A9 mRNA及其蛋白表达水平,并分析与不同肿瘤影像学表现如CT检查显示的骨质破坏程度,MRI检查显示的实性成分比例及周边水肿程度等关系。结果:43份骨巨细胞瘤组织中有40份表达S100A8、S100A9 mRNA及其蛋白,与正常骨髓组织比较,骨巨细胞瘤组织S100A8、S100A9 mRNA及其蛋白表达增加,差异均有统计学意义(均P<0.05)。影像学不同实质成分比例的骨巨细胞瘤患者S100A8蛋白表达差异具有统计学意义(P<0.05);不同骨质破坏程度的骨巨细胞瘤患者S100A9蛋白表达差异也有统计学意义(P<0.05)。结论:骨巨细胞瘤组织中S100A8和S100A9表达增加,并与骨巨细胞瘤骨质破坏程度及实性成分比例、水肿程度具有一定相关性,S100A8、S100A9可能参与骨巨细胞瘤的发生发展。

关键词: 巨细胞瘤,  骨/病理学,  巨细胞瘤,  骨/放射摄影术,  RNA,  信使/遗传学,  S100蛋白质类/生物合成,  体层摄影术,  X线计算机,  磁共振成像 

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