Quantitative determination and optimun extraction technique of nine compounds of <i>Paeoniae Radix Alba</i>

doi:10.3785/j.issn.1008-9292.2020.06.02

J Zhejiang Univ (Med Sci)

2020, Vol. 49

Issue (3): 356-363 DOI: 10.3785/j.issn.1008-9292.2020.06.02

Quantitative determination and optimun extraction technique of nine compounds of Paeoniae Radix Alba

FENG Yihan(

),YIN Lei,LIU Yuanrong,CAO Lujing,ZHENG Ning,LI Mingjuan*(

),ZHAN Shuyu*(

)

Medical College of Jiaxing University, Jiaxing 314001, Zhejiang Province, China

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Abstract

Objective: To establish the optimum extraction technique and high performance liquid chromatographic (HPLC) method to simultaneously quantify nine compounds of gallic acid, hydroxy-paeoniflorin, catechin, albiflorin, paeoniflorin, pentagalloylglucose, benzoic acid, benzoylpaeoniflorin and paeonol in Paeoniae Radix Alba. Methods: Linear gradient elution was applied using water containing 0.1%phosphoric acid and acetonitrile as the mobile phase with a flow rate of 0.8 mL/min, column temperature of 30℃ and wavelength of 230 nm. The method of ultrasound extraction was used. Methanol and ethanol were used as extraction solvents, and three factors and three levels of orthogonal experiments was designed using L₉(3⁴) table to investigate the effects of solvent concentration, ratio of liquid to material and extraction time on the total content of nine components of Paeoniae Radix Alba. Results: HPLC method was verified to have high specificity, sensitivity and accuracy through methodological validation, and it could be used for simultaneous quantitative analysis of nine components of Paeoniae Radix Alba. The results showed that the optimum extraction technology of nine components of Paeoniae Radix Alba was using 70%ethanol as extraction solvent, ratio of liquid to material was 200 mL/g and ultrasound extraction time was 30 min. Conclusion: HPLC method for the simultaneous determination of nine components of Paeoniae Radix Alba is established, and the optimum extraction technology is confirmed.

Key words： Paeonia Chromatography, liquid Technology, pharmaceutical Orthogonal experiment Ultrasound extraction

Received: 20 October 2019 Published: 24 July 2020

CLC:

R917

Corresponding Authors: LI Mingjuan,ZHAN Shuyu E-mail: 15900720995@163.com;limingjuan19860803@163.com;zhansy2000@163.com

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	FENG Yihan
	YIN Lei
	LIU Yuanrong
	CAO Lujing
	ZHENG Ning
	LI Mingjuan
	ZHAN Shuyu

Cite this article:

FENG Yihan,YIN Lei,LIU Yuanrong,CAO Lujing,ZHENG Ning,LI Mingjuan,ZHAN Shuyu. Quantitative determination and optimun extraction technique of nine compounds of Paeoniae Radix Alba. J Zhejiang Univ (Med Sci), 2020, 49(3): 356-363.

URL:

http://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2020.06.02 OR http://www.zjujournals.com/med/Y2020/V49/I3/356

杭白芍九种主要成分的高效液相色谱定量分析及提取工艺研究

目的: 建立同时测定杭白芍9种主要成分没食子酸、羟基芍药苷、儿茶素、芍药内酯苷、芍药苷、五没食子酰葡萄糖、苯甲酸、苯甲酰芍药苷和丹皮酚的高效液相色谱法（HPLC），优化杭白芍的提取工艺。方法: HPLC检测采用0.1%磷酸水溶液-乙腈为流动相线性梯度洗脱，流速为0.8 mL/min，柱温为30℃，检测波长为230 nm。采用超声提取法，甲醇和乙醇为提取溶剂，选用L₉（3⁴）表分别设计三因素三水平的正交试验，考察溶剂浓度、液料比和提取时间对杭白芍9种主要成分总含量的影响。结果: 所建立的HPLC法具有较高的专属性、灵敏度和准确性，可用于同时定量分析杭白芍9种主要成分；确定了杭白芍9种主要成分的最佳提取工艺，即采用70%乙醇为提取溶剂，液料比为200 mL/g，超声提取时间为30 min。结论: 建立了同时测定杭白芍9种主要成分的HPLC法及其最佳工艺条件。

关键词： 芍药属, 色谱法, 液相, 工艺学, 制药, 正交试验, 超声提取

Tab 1 Design of orthogonal experiments using methanol or ethanol as extraction solvent

Fig 1 High performance liquid chromatography of 70%ethanol (A), 70%methanol (B), mixed standard solution (C) and the prepared sample extracting from Paeoniae Radix Alba (D)

Tab 2 The linear and lower limit of quantification of nine components of Paeoniae Radix Alba

Tab 3 The results of orthogonal experiments with three factors and three levels for ultrasound extraction technology of Paeoniae Radix Alba

Tab 4 Range analysis of orthogonal experiment for ultrasound extraction technology of Paeoniae Radix Alba

Fig 2 The influence of solvent concentration, ratio of liquid to material and extraction time on the total content of nine components using methanol or ethanol as extraction solvent

Tab 5 Variance analysis of orthogonal experiment for ultrasound extraction technology of Paeoniae Radix Alba

Tab 6 The concentration of nine components extracting from Paeoniae Radix Alba using ethanol as extraction solvent ($\bar x \pm s$, n=3)


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