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J Zhejiang Univ (Med Sci)  2017, Vol. 46 Issue (1): 74-79    DOI: 10.3785/j.issn.1008-9292.2017.02.11
    
Antiendotoxin effect of Jinhuaqingre capsules
HOU Jiafu1(),WU Leilei1,CAI Yuting1,GAO Changjiu1,AN Jindan1,*(),YU Zhongcheng2,*()
(1) Mudanjiang Medical College, Mudanjiang 157011, China
(2) Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
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Abstract  Objective

To investigate the anti-pyretic and anti-endotoxin effect of Chinese herb medicine Jinhuaqingre capsules.

Methods

Thirty healthy male New Zealand rabbits with lipopolysaccharide-induced fever were divided into 5 groups (6 rabbits in each): animals in model group were given normal saline by gavage, animals in positive control group were given aspirin (0.2 g/kg), and animals in Jinhuaqingre groups were given Jinhuaqingre capsules 6.0, 3.0 or 1.5 g/kg, respectively. The changes in body temperature of rabbits were observed. Fifty healthy Kunming mice were divided into 5 groups (10 mice in each): mice in model group were given normal saline by gavage, mice in positive control group were given aspirin (0.2 g/kg), and those in Jinhuaqingre groups were given Jinhuaqingre capsules 6.0, 3.0, 1.5 g/kg, respectively. Matrix coloration method was used to detect the degradation rate of endotoxin in mice.

Results

The body temperature in rabbits of high and medium dose Jinhuaqingre capsule groups declined significantly 60 min after drug administration, and the temperature of high-dose group returned to the baseline after 300 min; while the body temperature of low-dose group started to decline at 180 min after drug administration. The endotoxin degradation rates in mice of high, medium and low dose groups was (56.73±3.12)%, (47.23±1.77)% and (21.08±2.30)% at 30 min after drug administration; those were (82.76±1.00)%, (64.75±1.77)% and (38.21±1.57)% at 60 min after drug administration, respectively.

Conclusion

Chinese herb medicine Jinhuanigre capsules have anti-pyretic and anti-endotoxin effects, which may provide a new option for the treatment of heat-toxin syndrome.



Key wordsFever/traditional Chinese medicine therapy      Disease models, animal      Endotoxins/adverse effects      Antipyretic agents/therapeutic use      Lipopolysaccharides     
Received: 15 December 2015      Published: 06 July 2017
CLC:  R285.5  
Corresponding Authors: AN Jindan,YU Zhongcheng     E-mail: 59101290@qq.com;jinandan@163.com;346953964@qq.com
About author: AN Jindan, E-mail:jinandan@163.com|YU Zhongcheng, E-mail:346953964@qq.com
Cite this article:

HOU Jiafu,WU Leilei,CAI Yuting,GAO Changjiu,AN Jindan,YU Zhongcheng. Antiendotoxin effect of Jinhuaqingre capsules. J Zhejiang Univ (Med Sci), 2017, 46(1): 74-79.

URL:

http://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2017.02.11     OR     http://www.zjujournals.com/med/Y2017/V46/I1/74


锦花清热胶囊抗内毒素作用实验研究

目的

探讨锦花清热胶囊的解热和抗内毒素作用。

方法

选健康雄性新西兰兔30只,采用注射脂多糖法建模,然后将所有新西兰兔分为五组:模型对照组(等渗氯化钠溶液),阳性对照组(阿司匹林0.2 g/kg),锦花清热胶囊大、中、小剂量组(分别含生药6.0、3.0、1.5 g/kg),每组6只,观察锦花清热胶囊对内毒素所致发热新西兰兔的降温作用;取昆明小鼠50只,分为五组:模型对照组(等渗氯化钠溶液),阳性对照组(阿司匹林0.2 g/kg),锦花清热胶囊大、中、小剂量组(分别含生药6.0、3.0、1.5 g/kg),每组10只,采用显色基质法检测锦花清热胶囊对昆明小鼠血浆中内毒素的降解率。

结果

新西兰兔解热实验结果显示,锦花清热胶囊大、中剂量组在60 min后降温作用显著,其中大剂量组在300 min时已经接近基础体温;小剂量组在180 min后开始有降温作用,提示锦花清热胶囊对发热新西兰兔有降温作用;昆明小鼠抗内毒素实验结果显示,锦花清热胶囊大、中、小剂量组在30 min时内毒素的降解率为(56.73±3.12)%、(47.23±1.77)%、(21.08±2.30)%,60 min时为(82.76±1.00)%、(64.75±1.77)%、(38.21±1.57)%,提示锦花清热胶囊对昆明小鼠有降低内毒素的作用。

结论

锦花清热胶囊有显著的解热和抗内毒素作用,可以为临床治疗热毒类疾病提供依据。


关键词: 发热/中药疗法,  疾病模型, 动物,  内毒素类/副作用,  清热解毒剂/治疗应用,  脂多糖类 
 Anti-pyretic effect of Jinhuaqingre capsules to New Zealand rabbits with lipopolysaccharide-induced fever
Fig 1 Chart showing the body temperature declining tendency
($\overline {x}$±s)
组别 n 30 min 60 min
吸光度值 内毒素浓度 (EU/mL) 内毒素降解率 (%) 吸光度值 内毒素浓度 (EU/mL) 内毒素降解率 (%)
模型对照组 5 0.718±0.010 0.869±0.013 0 0.661±0.013 0.793±0.017 0
阳性对照组 5 0.294±0.006 0.302±0.008 65.16±1.46 0.190±0.013 0.164±0.017 79.34±1.86
大剂量组 5 0.349±0.020 0.376±0.026 56.73±3.12 0.169±0.007 0.137±0.009 82.76±1.00
中剂量组 5 0.411±0.015 0.458±0.020 47.23±1.77 0.276±0.012 0.279±0.016 64.75±1.77
小剂量组 5 0.581±0.012 0.685±0.016 21.08±2.30 0.434±0.015 0.490±0.019 38.21±1.57
Tab 2 Matrix coloration method to examine the antiendotoxin effect of Jinhuaqingre capsules
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