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Journal of ZheJiang University(Medical Science)  2016, Vol. 45 Issue (5): 477-485    DOI: 10.3785/j.issn.1008-9292.2016.09.05
    
Effect of methyleugenol on expression of MUC5AC in nasal mucosa of rats with allergic rhinitis
MENG Nannan1, HOU Yun2, GUI Yan2, XI Kehu2, WANG Youhu2, YANG Jing2, CHEN Hong1, ZHANG Xiaobing2
1. Department of Child Care, the First Hospital of Lanzhou University, Lanzhou 730000, China;
2. Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Lanzhou University, Lanzhou 730000, China
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Abstract  

Objective: To investigate the effect of methyleugenol on expression of MUC5AC in nasal mucosa of rats with allergic rhinitis (AR). Methods: Seventy-two Wistar rats were randomly divided into 6 groups:normal control group, AR group, loratadine group, low-dose methyleugenol group, middle-dose methyleugenol group and high-dose methyleugenol group with 12 rats in each group. AR was induced by intraperitoneal injection of ovalbumin in latter 5 groups. 10 mg loratadine q.d was given to rats in loratadine group by gavage; and 10 mg/kg, 20 mg/kg and 40 mg/kg methyleugenol were given by gavege q.d to rats in low-, middle-and high-dose methyleugenol groups, respectively. Nasal mucosa samples were obtained from rats at 1, 2, 4 and 6 weeks after drug intervention. The expression of MUC5AC protein and mRNA in nasal mucosa was detected by immunohistochemistry and real-time fluorescence quota PCR (RT-PCR), respectively. Results: Compared with AR, the percentage of cells staining positively for MUC5AC protein and the relative quantity of MUC5AC mRNA in middle-and high-dose methyleugenol groups were significantly decreased after 2 and 4 weeks of drug intervention (P<0.05), but no such decrease was observed in low-dose methyleugenol group at all time points (P>0.05). The percentage of cells with positive expression of MUC5AC protein and mRNA in loratadine group were significantly decreased after 1 week of administration (P<0.05). The percentage of cells with positive MUC5AC protein in middle-dose methyleugenol group was higher than that in loratadine group (P<0.05) after 6 week of drug intervention, but the difference was not seen in high-dose group (P>0.05). There was no significant difference in relative quantities of MUC5AC mRNA after 4 weeks of administration between high-and middle-dose methyeugenol groups and loratadine group (P>0.05). Conclusion: Methyleugenol can attenuate AR through inhibiting the expression of MUC5AC mRNA and protein in nasal mucosa of AR rats.



Key wordsEugenol/pharmacology      Eugenol/administration &      dosage      Rhinitis/drug therapy      Loratadine/therapeutic use      Mucins/drug effects      Nasal mucosa/drug effects      Rats, Wistar     
Received: 01 October 2015      Published: 25 September 2016
CLC:  R966  
  R593.1  
Cite this article:

MENG Nannan, HOU Yun, GUI Yan, XI Kehu, WANG Youhu, YANG Jing, CHEN Hong, ZHANG Xiaobing. Effect of methyleugenol on expression of MUC5AC in nasal mucosa of rats with allergic rhinitis. Journal of ZheJiang University(Medical Science), 2016, 45(5): 477-485.

URL:

http://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2016.09.05     OR     http://www.zjujournals.com/med/Y2016/V45/I5/477


甲基丁香酚对变应性鼻炎大鼠鼻黏膜组织中黏蛋白5AC的影响

目的:观察甲基丁香酚对变应性鼻炎大鼠鼻黏膜中黏蛋白5AC(MUC5AC)的影响。方法:将72只Wistar大鼠分为正常对照组(12只)和造模组(60只),卵清蛋白致敏Wistar大鼠建立变态性鼻炎动物模型。造模成功后,大鼠随机分为模型对照组、氯雷他定组和甲基丁香酚小、中、大剂量组(10 mg/kg、20 mg/kg、40 mg/kg),各组给药干预,分别在干预1周、2周、4周和6周后取材,采用免疫组织化学检测和实时定量PCR检测MUC5AC mRNA及其蛋白的表达。结果:MUC5AC蛋白阳性表达细胞百分率及MUC5AC mRNA表达量小剂量组在整个干预过程中与模型对照组差异无统计学意义(均P>0.05);大、中剂量组分别在干预2周和4周后明显减少,差异均有统计学意义(均P<0.05);氯雷他定组在干预1周后明显减少,差异有统计学意义(P<0.05)。与氯雷他定组MUC5AC蛋白阳性表达细胞百分率比较:中剂量组在干预6周后依然较高(P<0.05),大剂量组在干预6周后差异无统计学意义(P>0.05)。与氯雷他定组MUC5AC mRNA表达量比较,大、中剂量组在干预4周后差异均无统计学意义(均P>0.05)。结论:甲基丁香酚可以显著抑制变应性鼻炎大鼠鼻黏膜中黏蛋白MUC5AC的表达,进而对变应性鼻炎有一定的抑制作用,可为临床应用研究提供依据。


关键词: 丁香酚/药理学,  丁香酚/投药和剂量,  鼻炎/药物疗法,  氯雷他定/治疗应用,  黏蛋白类/药物作用,  鼻黏膜/药物作用,  大鼠, Wistar 
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