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Journal of ZheJiang University(Medical Science)  2016, Vol. 45 Issue (2): 126-131    DOI: 10.3785/j.issn.1008-9292.2016.03.04
Fabrication of bioactive tissue engineering scaffold for reconstructing calcified cartilage layer based on three-dimension printing technique
YU Xinning1, FANG Jinghua1, LUO Jianyang1, YANG Xianyan2, HE Dongshuang2, GOU Zhongru2, DAI Xuesong1
1. Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China;
2. Zhejiang-California International Nanosystems Institute, Hangzhou 310058, China
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Objective: To fabricate organic-inorganic composite tissue engineering scaffolds for reconstructing calcified cartilage layer based on three-dimensional (3D) printing technique. Methods: The scaffolds were developed by 3D-printing technique with highly bioactive calcium-magnesium silicate ultrafine particles of 1%, 3% and 5% of mass fraction, in which the organic phases were composed of type I collagen and sodium hyaluronate. The 3D-printed scaffolds were then crosslinked and solidified by alginate and CaCl2 aerosol. The pore size and distribution of inorganic phase were observed with scanning electron microscope (SEM); the mechanical properties were tested with universal material testing machine, and the porosity of scaffolds was also measured. Results: Pore size was approximately (212.3±34.2) μm with a porosity of (48.3±5.9)%, the compressive modulus of the scaffolds was (7.2±1.2) MPa, which was irrelevant to the percentage changes of calcium-magnesium silicate, the compressive modulus was between that of cartilage and subchondral bone. Conclusion: The porous scaffolds for calcified cartilage layer have been successfully fabricated, which would be used for multi-layered composite scaffolds in osteochondral injury.

Key wordsCartilage diseases/therapy      Silicon      Calcium compounds      Hyaluronic acid      Collagen type I      Computer-aided design      Imaging, three-dimensional      Sodiwm alginate      Scaffolds     
Received: 12 October 2015     
CLC:  R68  
Cite this article:

YU Xinning, FANG Jinghua, LUO Jianyang, YANG Xianyan, HE Dongshuang, GOU Zhongru, DAI Xuesong. Fabrication of bioactive tissue engineering scaffold for reconstructing calcified cartilage layer based on three-dimension printing technique. Journal of ZheJiang University(Medical Science), 2016, 45(2): 126-131.

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目的: 初步构建基于软骨钙化层损伤重建的有机—无机复合组织工程支架,探究掺镁硅灰石含量与支架抗压性能之间的关系。方法: 利用质量分数分别为1%、3%、5%的高生物活性钙镁硅酸盐超细颗粒复合Ⅰ型胶原—透明质酸钠进行三维打印,经海藻酸钠—氯化钙气雾交联成型,电镜下观察表面孔隙、孔径、无机相分布,万能材料试验机测试抗压性能,并计算支架孔隙率。结果: 支架表面平均孔径(212.3±34.2)μm,平均孔隙率(48.3±5.9)%,不同质量分数的高生物活性钙镁硅酸盐超细颗粒复合Ⅰ型胶原—透明质酸钠支架压缩模量差异无统计学意义(P>0.05),平均压缩模量(7.2±1.2)MPa,介于软骨和软骨下骨之间。结论: 利用三维打印技术成功构建出多孔钙化层仿生重建支架,可为今后研制多层次复合支架治疗骨—软骨损伤奠定基础。

关键词: 软骨疾病/治疗,  硅,  钙化合物,  透明质酸,  胶原Ⅰ型,  计算机辅助设计,  成像,三维,  海藻酸钠,  支架 

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