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浙江大学学报（医学版）
Journal of ZheJiang University(Medical Science)  2015, Vol. 44 Issue (2): 162-166    DOI: 10.3785/j.issn.1008-9292.2015.03.007
    
Effect of irradiated human lung fibroblasts on activation of canonical Wnt/β-catenin signaling pathway in mesenchymal stem cells
ZHANG Chun-yang1,2, ZHU Yan2, FENG Hua-song2, CHEN Xu-xin2
1. Graduate Student Team of Medical School of Chinese PLA, Beijing 100853, China;
2. Department of Respiratory Medicine, Navy General Hospital, Beijing 100048, China
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Abstract  

Objective: To investigate the effect of irradiated human lung fibroblasts (HLFs) on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells (HUMSCs). Methods: HUMSCs were cultured alone (single group) or co-cultured with HLFs exposed to 5Gy X-rays (co-culture group). The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture. WISP-1 protein levels in conditioned medium were examined by ELISA. Results: The levels of p-GSK3β/GSK3β(0.15±0.05), FRAT1(0.48±0.07) and β-catenin(0.50±0.07)in co-cultured HUMSCs significantly decreased compared to those in single group (0.55±0.05, 1.16±0.13 and 2.39±0.15, all P<0.05).The supernatant level of WISP-1 in co-culture group was significantly decreased[(602.23±161.47)ng/mL], compared to the single group[(977.77±110.56)ng/mL, P<0.05].Conclusion: Irradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway in HUMSCs in vitro.

Key words： Fibroblasts      Mesenchymal stem cells/physiology      Umbilical cord      Radiation pneumonitis      Wnt proteins/biosynthesis      Beta-catenin/biosynthesis      Intercellular signaling peptides and proteins      Signal transduction     
Received: 17 November 2014      Published: 25 March 2015
CLC:  R146  
  Q691.4  
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ZHANG Chun-yang
ZHU Yan
FENG Hua-song
CHEN Xu-xin
Cite this article:

ZHANG Chun-yang, ZHU Yan, FENG Hua-song, CHEN Xu-xin. Effect of irradiated human lung fibroblasts on activation of canonical Wnt/β-catenin signaling pathway in mesenchymal stem cells. Journal of ZheJiang University(Medical Science), 2015, 44(2): 162-166.

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http://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2015.03.007     OR     http://www.zjujournals.com/med/Y2015/V44/I2/162


放射线照射的肺成纤维细胞对人脐带间充质干细胞中经典Wnt/β-catenin通路的影响

目的:体外研究放射线照射后的人肺成纤维细胞(HLFs)对人脐带间充质干细胞(HUMSCs)中经典Wnt/β-catenin信号通路的影响。方法:实验细胞分为共培养组和单纯HUMSCs组,共培养组为5Gy X射线照射后的HLFs通过Transwell系统与HUMSCs共培养。细胞培养3d后,蛋白质印迹法检测HUMSCs中GSK-3β、p-GSK-3β、FRAT1和胞核内β-catenin蛋白表达,酶联免疫吸附试验检测细胞培养上清液中WISP-1蛋白表达。 结果:共培养组HUMSCs中p-GSK-3β/GSK-3β相对比值(0.15±0.05)和FRAT1表达水平(0.48±0.07)较单纯HUMSCs组(0.55±0.05和1.16±0.13)明显下降,共培养组HUMSCs胞核内β-catenin(0.50±0.07)也较单纯HUMSCs组(2.39±0.15)明显下降;共培养组HUMSCs培养上清液中WISP-1蛋白表达[(602.23±161.47)ng/mL]较单纯HUMSCs组[(977.77±110.56)ng/mL]明显下降,差异均有统计学意义(均P<0.05)。结论:放射线照射后的HLFs对HUMSCs中经典Wnt/β-catenin通路具有抑制作用。


关键词： 成纤维细胞,  间质干细胞/生理学,  脐带,  辐射性肺炎,  Wnt蛋白质类/生物合成,  β连环素/生物合成,  胞间信号肽类和蛋白质类,  信号传导 

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