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Journal of ZheJiang University(Medical Science)  2014, Vol. 43 Issue (2): 175-179    DOI: 10.3785/j.issn.1008-9292.2014.03.010
    
Purification of polyphenols from sabina vulgaris antoine and its antioxidant properties
LI Long1,2, XUAN Gui-da1 ,CHEN Ping3
1. School of Medicine, Zhejiang University City College, Hangzhou 310015,China; 2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310018, China; 3. College of Science, Zhejiang University, Hangzhou 310018, China
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Abstract  

Objective: To purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties. Methods: Polyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700, and the quantity of polyphenols was determined by    Folin-Ciocalteu colorimetry. The antioxidant properties of polyphenols were evaluated by total antioxidant capacity (T-AOC) and its activeties of scavenging DPPH (1,1diphenyl-2-picry-hydrazyl) radicals, superoxide anion (O2·-), hydroxyl free radicals (OH·) and ferric     reducing antioxidant power (FRAP). Results: After purification, the purity of polyphenols increased from 0.53‰ to 9.95‰.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml. Its scavenging capacity for superoxide anion (O2·-) and hydroxyl free radicals (OH·) was 151.83 U/ml and 204.59 U/ml. The total antioxidant capacity  was 72.68 U/ml. Conclusion: Polyphenols from Sabina vulgaris Antoine have high antioxidant properties, suggesting that it worth further study of its pharmacological effects.



Key wordsCupressaceae/chemistry      Phenols      Antioxidants     
Received: 20 August 2013     
Corresponding Authors: XUAN Gui-da, E-mail: xuangd@zucc.edu.cn   
Cite this article:

LI Long, XUAN Gui-da,CHEN Ping. Purification of polyphenols from sabina vulgaris antoine and its antioxidant properties. Journal of ZheJiang University(Medical Science), 2014, 43(2): 175-179.

URL:

http://www.zjujournals.com/xueshu/med/10.3785/j.issn.1008-9292.2014.03.010     OR     http://www.zjujournals.com/xueshu/med/Y2014/V43/I2/175


沙地柏多酚的纯化及其抗氧化活性测定

目的:提高沙地柏多酚的纯化程度并考察其抗氧化活性。方法:采用Folin-Ciocalteu法测定沙地柏的多酚含量,经筛选用HPD-700大孔树脂纯化沙地柏多酚。通过对沙地柏多酚,1,1-二苯基-2-三硝基苯肼(DPPH)自由基、超氧阴离子、羟自由基的清除能力、总抗氧化能力及三价铁离子(Fe3+)还原能力的测定来评价其抗氧化能力。结果:经纯化后,沙地柏多酚纯度由0.53‰提高到9.95‰。抗氧化活性测定结果表明,沙地柏多酚对DPPH自由基的抑制率和Fe3+还原能力的维生素C当量质量浓度分别为9.4 μg/ml、30.09 μg/ml,对超氧阴离子和羟自由基的清除能力分别为151.83 U/ml、204.59 U/ml,总抗氧化能力为72.68 U/ml。结论:沙地柏多酚具有较强的抗氧化活性,值得进一步研究其药理作用。


关键词: 柏科/化学,  酚类,  抗氧化剂 
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