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Journal of ZheJiang University(Medical Science)  2014, Vol. 43 Issue (2): 150-154    DOI: 10.3785/j.issn.1008-9292.2014.03.005
    
Determination of enantiomeric impurity in levocetirizine tablets by capillary electrophoresis
WANG Shi-zhuo, ZHAO Yun-feng, SUN Jia-yi, GUO Xing-jie
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
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Abstract  

Objective: To determine enantiomeric impurity in levocetirizine tablets by using capillary electrophoresis.Methods: The effects of pH and the concentrations of sulfated-β-cyclodextrin (S-β-CD) and buffer salt on chiral resolution were examined with S-β-CD as chiral selector. Results: A good enantioseparation of cetirizine was achieved with 30 mmol/L NaH2PO4 buffer solution (pH 7.0) containing 20 g/L of S-β-CD.Conclusion: The method developed in the study is sensitive and reliable for determination of enantiomeric impurity in levocetirizine tablets.



Key wordsCetirizine/analysis      Cetirizine/analogs &      derivatives      Electrophoresis      capillary/methods      beta-Cyclodextrins/chemistry      Stereoisomerism     
Received: 29 October 2013     
Corresponding Authors: GUO Xing-jie, E-mail: syykdx410@163.com   
Cite this article:

WANG Shi-zhuo, ZHAO Yun-feng, SUN Jia-yi, GUO Xing-jie. Determination of enantiomeric impurity in levocetirizine tablets by capillary electrophoresis. Journal of ZheJiang University(Medical Science), 2014, 43(2): 150-154.

URL:

http://www.zjujournals.com/xueshu/med/10.3785/j.issn.1008-9292.2014.03.005     OR     http://www.zjujournals.com/xueshu/med/Y2014/V43/I2/150


毛细管电泳法检查盐酸左西替利嗪片中右旋异构体杂质

目的:建立毛细管电泳法检查盐酸左西替利嗪片中右旋异构体杂质。方法:用磺酸化-β-环糊精(S-β-CD)作为手性选择剂,考察了S-β-CD浓度、pH值和缓冲盐浓度对分离的影响。结果:在30 mmol/L磷酸盐运行缓冲液(含20 g/L S-β-CD,pH 7.0)中,西替利嗪对映体在分离电压为15 kV的条件下得到良好分离,并满足对盐酸左西替利嗪片检查右旋异构体杂质所需要的条件。结论:本实验建立的方法灵敏、可靠,可用于左旋西替利嗪中右旋异构体杂质的检查。


关键词: 西替利嗪/分析,  西替利嗪/类似物和衍生物,  电泳,  毛细管/方法,  &beta,  环糊精类/化学,  立体异构现象 
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