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Journal of ZheJiang University(Medical Science)  2014, Vol. 43 Issue (2): 145-149    DOI: 10.3785/j.issn.1008-9292.2014.03.004
    
Determination of R(-)- isomer in repaglinide tablets by capillary electrophoresis
YUAN Xiao-wei, SUN Jia-yi, WANG Shi-zhuo, GUO Xing-jie
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
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Abstract  

Objective: To develop a capillary electrophoresis system for enantiomeric impurity test of repaglinide.Methods: An uncoated fused silica capillary (50 μm×50 cm, with an effective length of 41 cm) was used. The running buffer was composed of 30 mmol/L NaH2PO4 and 5 mg/ml carboxymethyl-β-cyclodextrin(pH 3.5). Results: Linear range was 2.00-80.00  μg/ml (correlation coefficient was 0.9993). The average recovery rate was 92.5% to 105.0%. Conclusion: The method is simple, accurate and sensitive and it can be used for determination of enantiomeric impurities in repaglinide tablet.



Key wordsCarbamates/analysis      Carbamates/analogs &      derivatives      Electrophoresis              capillary/methods      Stereoisomerism      Repaglinide     
Received: 30 December 2013     
Corresponding Authors: GUO Xing-jie, E-mail: syykdx410@163.com   
Cite this article:

YUAN Xiao-wei, SUN Jia-yi, WANG Shi-zhuo, GUO Xing-jie. Determination of R(-)- isomer in repaglinide tablets by capillary electrophoresis. Journal of ZheJiang University(Medical Science), 2014, 43(2): 145-149.

URL:

http://www.zjujournals.com/xueshu/med/10.3785/j.issn.1008-9292.2014.03.004     OR     http://www.zjujournals.com/xueshu/med/Y2014/V43/I2/145


毛细管电泳法检查瑞格列奈片中左旋异构体杂质

目的:建立毛细管电泳法检测瑞格列奈片中左旋异构体杂质。方法:采用未涂层熔融石英毛细管柱(50 μm×50 cm,有效长度41 cm)为分离通道,运行缓冲液为pH 3.5, 30 mmol/L的磷酸二氢钠缓冲溶液,含5 mg/ml CM-β-CD。结果:左旋瑞格列奈在浓度为2.00~80.00 μg/ml范围内线性关系良好 (r=0.9993),方法回收率为92.5%~105.0%。结论:本实验建立的方法简单、准确、灵敏,可用于瑞格列奈片中左旋异构体杂质的检测。


关键词: 氨甲酸酯类/分析,  氨甲酸酯类/类似物和衍生物,  电泳,  毛细管/方法,  立体异构现象,  瑞格列奈 
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