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| Effects of chrysalis oil on learning，memory and oxidative stress in D-galactose-induced ageing model of mice |
| CHEN Weiping，YANG Qiongjie，WEI Xing |
| Zhejiang University City College，School of Medicine，Hangzhou 310015 |
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Abstract Objective： To investigate the effects of chrysalis oil on learning，memory and oxidative stress in D-galactose-induced ageing model of mice.
Methods： Mice were injected intraperitoneally with D-galactose daily and received chrysalis oil intragastrically simultaneously for 30 d.Then mice underwent space navigation test and spatial probe test，superoxide dismutase (SOD)，glutathione peroxidase (GSH-PX) activity and malondialdehyde (MDA) contents in mouse brain were measured.
Results： Compared to model group，escape latency in mice treated with 6 ml/kg·d chrysalis oil was significantly shorter (P<0.05)，crossing times in 12 ml/kg·d group and 6 ml/kg·d group treated with chrysalis oil were significantly increased (P <0.05).Chrysalis oil treatment (12ml/kg·d) significantly increased SOD and GSH-PX activity and reduced MDA contents in brain of D-galactose-induced aging mice.
Conclusion： Chrysalis oil can improve the ability of learning and memory in D-galactose-induced aging mice，and inhibit peroxidation in brain tissue.
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Received: 20 August 2013
Published: 25 November 2013
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蚕蛹油对D-半乳糖模型小鼠学习记忆及氧化应激的影响
目的：观察蚕蛹油对D-半乳糖致衰老小鼠学习记忆能力及机体抗氧化能力的影响，为蚕蛹油的临床应用积累资料。
方法：连续给小鼠腹腔注射D-半乳糖30 天，同时用蚕蛹油灌胃给药，给药结束后用Morris水迷宫进行4天的定位航行试验，测定逃避潜伏期；第5天进行空间搜索试验，测定其穿越平台次数；并比较了各组小鼠组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）活性和丙二醛（MDA）含量。
结果：与模型组比较，蚕蛹油6 ml/kg·d组逃避潜伏期明显短于模型组（P<0.05）；蚕蛹油6 ml/kg·d组与12 ml/kg·d组穿越平台次数明显多于模型组（P<0.05）；蚕蛹油12 ml/kg·d组能显著提高D-半乳糖所致衰老小鼠脑组织SOD、GSH-PX活性，降低脂质过氧化产物MDA的含量。
结论：蚕蛹油能改善D-半乳糖致衰老小鼠的学习记忆能力，有抑制其脑组织脂质过氧化的作用。
关键词：
蛹,
蚕属,
半乳糖/药理学,
氧化性应激,
记忆,
随机对照试验,
蚕蛹油,
D-半乳糖,
学习记忆,
抗氧化
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