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Journal of ZheJiang University(Medical Science)  2013, Vol. 42 Issue (4): 418-    DOI: 10.3785/j.issn.1008-9292.2013.04.008
Effect of Xinfeng Capsule on lung function in rats with adjuvant-induced arthritis and its mechanism
WAN Lei,LIU Jian,HUANG Chuanbing,WANG Yuan,SHEN Xi,ZHANG Wandong,WANG Guizheng,FAN Haixia,GE Yao,CHEN Ruilian,CAO Yunxiang,ZONG Ruikai
Department of Rheumatology,The First Affiliated Hospital,Anhui University of Chinese Medicine,Hefei 230031,China
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Abstract]Objective: To investigate the effects of Xinfeng Capsule (XFC) on pulmonary function and related mechanism in adjuvant-induced arthritis (AA) rats. Methods: The rats were randomly divided into five groups:normal control (NC),model control (MC) groups,methotrexate (MTX),tripterygium glycosides tablet (TPT) and Xinfeng capsule (XFC) treatment groups.The adjuvant-induced arthritis model was established by intracutaneous injection of 0.1 mL Freund′s complete adjuvant in the right paw of rats; the drugs were given 19 d after model establishment.The toe swelling degree (E),arthritis index (AI),pulmonary function,peripheral blood Treg levels,pathological changes of lung tissue and expression of Foxp3,TGF-β1,Smad3,Smad7 proteins in lung tissue were measured 30 d after drug administration. Results: Compared to NC group,the levels of E,AI,alveolitis score,TGF-β1 and Smad3 were significantly increased (P<0.05 or P<0.01);maximum expiratory flow 25% of vital capacity (FEF25),50% maximal expiratory vital capacity flow (FEF50),maximum expiratory flow at 75% of vital capacity (FEF75),maximum mid-expiratory flow (MMF),force peak expiratory flow (PEF),CD4+ CD25+ Treg,Foxp3 and Smad7 were significantly decreased in MC group (P<0.05 or P<0.01).Compared to MC group,the expression of E,AI,TGF-β1 and Smad3 were reduced,while FEF50,FEF75,MMF,PEF,Treg,Foxp3 and Smad7 were elevated in XFC group (P<0.05 or P<0.01).Compared to XFC group,the level of body mass,FEF25,FEF50,FEF75,MMF and Treg were lower in MTX and TPT groups (P<0.05 or P<0.01). Conclusions: There are inflamed joints and reduced pulmonary function in rats of adjuvant-induced arthritis.XFC can inhibit paw edema degrees,reduce arthritis response,and improve pulmonary function,which is associated with up-regulating expression of Treg and Foxp3, down-regulating the expression of TGF-β1 and adjusting TGF-β1/Smads signal pathway.

Key wordsArthritis,experimental/drug therapy      Respiratory function tests      Capsules/therapeutic use      Adjuvant arthritis      Pulmonary function      Regulatory T cells      TGF-β1/Smads pathway      Xinfeng Capsule      Disease models,animal     
Received: 17 December 2012      Published: 07 August 2013
CLC:  R 593.22  
Cite this article:

WAN Lei,LIU Jian,HUANG Chuanbing,WANG Yuan,SHEN Xi,ZHANG Wandong,WANG Guizheng,F. Effect of Xinfeng Capsule on lung function in rats with adjuvant-induced arthritis and its mechanism. Journal of ZheJiang University(Medical Science), 2013, 42(4): 418-.

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[摘要]目的:观察新风胶囊(XFC)对佐剂关节炎(AA)大鼠肺功能、调节T细胞(Treg)及肺组织Foxp3、TGF-β1、Smad3、Smad7的影响。方法:采用弗氏完全佐剂复制AA大鼠模型,致炎后第19天开始给药,将大鼠随机均分为正常对照(NC)组、模型对照(MC)组、甲氨喋呤(MTX)组、雷公藤多苷片(TPT)组和XFC组,给药30 d后,观察各组大鼠足趾肿胀度(E)、关节炎指数(AI)、肺功能、Treg、肺组织病理形态学及肺组织Foxp3、TGF-β1、Smad3、Smad7变化。结果:与NC组相比,MC组大鼠E、AI、肺泡炎积分、TGF-β1及Smad3蛋白表达明显升高(P<0.05或P<0.01);25%肺活量的最大呼气流量(FEF25)、50%肺活量的最大呼气流量(FEF50)、75%肺活量的最大呼气流量(FEF75)、最大呼气中期流量(MMF)、用力最大呼气流量(PEF)、CD4+CD25+Treg及Foxp3、Smad7蛋白表达显著降低(P<0.05或P<0.01)。与MC组相比,XFC组大鼠E、AI、TGF-β1及Smad3表达降低,FEF50、FEF75、MMF、PEF、Treg及Foxp3、Smad7表达升高(P<0.05或P<0.01)。与XFC组相比,MTX组、TPT组大鼠体质量、FEF25、FEF50、FEF75、MMF、Treg降低(P<0.05或P<0.01)。结论:AA大鼠存在关节炎症症状和肺功能降低,XFC能明显抑制AA大鼠足跖肿胀度,降低关节炎症反应,改善肺功能,机制可能是通过上调Foxp3、Treg,下调TGF-β1表达,调节TGF-β1/Smads信号通路传导,改善关节症状和肺功能。

关键词: 关节炎,  实验性/药物疗法,  呼吸功能试验,  胶囊/治疗应用,  佐剂关节炎,  肺功能,  调节性T细胞,  TGF-&beta,  1/Smads通路,  新风胶囊,  疾病模型,  动物 
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