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Journal of ZheJiang University(Medical Science)  2013, Vol. 42 Issue (3): 331-336    DOI: 10.3785/j.issn.1008-9292.2013.03.013
    
Biological characteristics of phage SM1 for Stenotrophomonas maltophilia and its effect in animal infection model
ZHANG Jie1, LI Xiu-Juan2
1.Department of Geriatrics Medicine,the Third People′s Hospital of Chongqing,Chongqing 400014,China; 2.Department of General Internal Medicine,the First Branch of the First Affiliated Hospital of Chongqing Medical University,Chongqing 400015,China
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Abstract  

Objective: To investigate the biological characteristics of phage SM1 for stenotrophomonas maltophilia (Sm) and its effect in animal infection model. Methods: Phage SM1 isolated from raw sewage of hospital was identified by the plaque method.The morphology of phage SM1 was observed by electronmicrographics with negative staining.The extraction and electrophoresis of phage SM1 DNA were performed.Optimal multiplicity of infection,resistant mutation rate,one step growth curve and the effectiveness in animal models of phage SM1 were determined. Results: One Sm specific phage of myoviridae double-stranded DNA was identified,and named SM1.Electrophoresis of DNA demonstrated that the size of phage SM1 genome was about 50 kb.The growth curve of phage SM1 showed that the durations of incubation and burst period were 15 min and 50 min,respectively; and the burst size was 187.The resistant mutation rate of phage SM1 was 6×10-10.All mice treated with phage SM1 survived after 7 d of infection with stenotrophomonas maltophilia. Conclusions: The phage SM1 has a relatively broad host range,a shorter incubation period,an apparent burst size and a lower resistant mutation rate.The therapy of phage SM1 for Sm infection in mice is effective.



Key wordsStenotrophomonas maltophilia/drug effects      Stenotrophomonas maltophilia/enzymology      Bacteriophages/isolation &      purification      Models      animal      Anti-bacterial agents/therapeutic use     
Received: 15 March 2012      Published: 25 May 2013
CLC:  R 378  
Cite this article:

ZHANG Jie, LI Xiu-Juan. Biological characteristics of phage SM1 for Stenotrophomonas maltophilia and its effect in animal infection model. Journal of ZheJiang University(Medical Science), 2013, 42(3): 331-336.

URL:

https://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2013.03.013     OR     https://www.zjujournals.com/med/Y2013/V42/I3/331


嗜麦芽窄食单胞菌噬菌体SM1生物学特征及其在感染动物模型中的疗效

目的:通过分离嗜麦芽窄食单胞菌(Sm)噬菌体,研究噬菌体SM1的生物学特征及其在感染动物模型中的疗效,为噬菌体治疗Sm感染提供实验依据。方法:利用噬菌斑法从医院污水中分离噬菌体SM1,电镜观察其形态特征,并提取基因组行酶切电泳;测定噬菌体SM1最佳感染复数、耐受突变率、一步生长曲线及其在感染动物模型中的作用。结果:筛选出1株具有较宽裂解谱的双链DNA肌尾噬菌体SM1,基因组大小约50 kb;其感染宿主菌的潜伏期为15 min,爆发期为50 min,裂解量为187,耐受突变率为6×10-10;噬菌体SM1治疗组小鼠7 d后全部存活。结论:噬菌体SM1具有裂解谱较宽、潜伏期短、裂解量大及耐受突变率低的特点,而且可有效治疗Sm感染的小鼠。


关键词: 嗜麦芽窄食单胞菌/药物作用,  嗜麦芽窄食单胞菌/酶学,  细菌噬菌体/分离和提纯,  模型,  动物,  抗菌药/治疗应用 
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