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Journal of ZheJiang University(Medical Science)  2013, Vol. 42 Issue (2): 177-183    DOI: 10.3785/j.issn.1008-9292.2013.02.008
    
Vasodilating effect of capsaicin on rat mesenteric artery and its mechanism
CHEN Qiang1,2, ZHU Huan-Huan1, ZHANG Yuan-Yuan1, ZHANG Yuan1, WANG Li-Hong1, ZHENG Liang-Rong1
1.Department of Cardiology,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China; 2.Department of Cardiology,The Yiwu Affiliated Hospital,Zhejiang University School of Medicine,Yiwu 322000,China)
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Abstract  

Objective: To investigate the vasodilating effect of capsaicin(CAP) on rat mesenteric artery and its mechanism.
Methods: The third branch of the superior mesenteric artery in male Sprague-Dawley rat(250-350 g) was excised,the periadventitial fat and connective tissue were removed and the mesenteric artery was dissected into 2 mm rings.Each ring was placed in a 5 ml organ bath of DMT 610M system and the tension was recorded.
Results: CAP(10-9-10-5 mol/L) relaxed endothelium-intact and endothelium-denuded mesenteric artery pre-constricted by phenylephrine(10-5 mol/L),and the vasodilation in endothelium-intact mesenteric artery was stronger than that in endothelium-denuded one.Pretreatment with either L-NAME(3×10-4 mol/L),an inhibitor of nitric oxide synthase(NOS), or CGRP8-37(2×10-6 mol/L),an antagonist of calcitonin gene-related peptide(CGRP),for 30 min significantly attenuated the relaxation of endothelium-intact mesenteric artery induced by CAP.CGRP(10-10-3×10-8 mol/L) relaxed endothelium-intact and endothelium-denuded mesenteric artery pre-constricted by phenylephrine,and the vasodilation in endothelium-intact mesenteric artery was stronger than that in endothelium-denuded one.Substance P did not relax the mesenteric artery pre-constricted by phenylephrine.
Conclusions: CAP has partial endothelium-dependent relaxation effect on rat mesenteric artery,which may be mediated by activating the endothelial NOS-NO pathway.The endothelium-independent relaxation in rat mesenteric artery induced by CAP may be mediated by CGRP.



Key wordsCapsaicin/pharmacology      Mesenteric arteries/drug effects      Vasodilation      Calcitonin gene-related peptide      Endothelium     
Received: 27 April 2012      Published: 25 March 2013
CLC:  R 331.3  
Cite this article:

CHEN Qiang, ZHU Huan-Huan, ZHANG Yuan-Yuan, ZHANG Yuan, WANG Li-Hong, ZHENG Liang-Rong. Vasodilating effect of capsaicin on rat mesenteric artery and its mechanism. Journal of ZheJiang University(Medical Science), 2013, 42(2): 177-183.

URL:

http://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2013.02.008     OR     http://www.zjujournals.com/med/Y2013/V42/I2/177


辣椒素对大鼠肠系膜阻力血管的舒张作用及其机制

目的:观测辣椒素(capsaicin,CAP)对大鼠肠系膜阻力血管的舒张作用,探讨其可能机制。
方法:雄性Sprague-Dawley大鼠,取肠系膜动脉三级分支约2 mm长血管环,置于DMT 610M系统,记录张力变化。
结果:CAP(10-9~10-5 mol/L)对苯肾上腺素(PE)预收缩的内皮完整血管和去内皮血管产生浓度依赖性的舒张作用,其中内皮完整组的血管舒张作用比去内皮组更明显,两者相比CAP在10-7~10-5 mol/L时,差异有统计学意义(P<0.01)。该舒张作用可被一氧化氮合酶抑制剂L-NAME和CGRP竞争性阻滞剂CGRP8-37阻断。CGRP(10-10~3×10-8 mol/L)对PE预收缩的内皮完整血管和去内皮血管产生浓度依赖性的舒张作用,其中内皮完整组的血管舒张作用比去内皮组更明显,两者相比CGRP在3×10-9~3×10-8 mol/L时,差异有统计学意义(P<0.05)。P物质对PE预收缩的内皮完整血管无舒张作用。
结论:CAP对大鼠肠系膜阻力动脉具有部分内皮依赖性舒张作用,该作用机制与其促进内皮一氧化氮合酶(NOS)途径有关;CAP的非内皮依赖性舒张血管效应与CGRP释放有关。


关键词: 辣椒素/药理学,  肠系膜动脉/药物作用,  血管舒张,  降钙素基因相关肽,  内皮 

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